Date published: 2025-9-8
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HNF-3β Antibody (RY-7) - Western Blotting - Image 283496
HNF-3β (RY-7): sc-101060. Hep G2 (A) 和 A549 (B) 核提取物中 HNF-3β 表达的 Western 印迹分析.
HNF-3β (RY-7): sc-101060. Hep G2 (A) 和 A549 (B) 核提取物中 HNF-3β 表达的 Western 印迹分析.

HNF-3β 抗体 (RY-7): sc-101060

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说明书
  • HNF-3β抗体(RY-7)是小鼠单克隆IgG2a κ, 在61篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组HNF-3β
  • 推荐用于 mouse, rat 和 human 来源的HNF-3β WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 HNF-3β (H-4): sc-374376，可查看有标记的 HNF-3β 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取HNF-3β (RY-7): sc-101060的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 HNF-3β 抗体 (RY-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HNF-3β 抗体 (RY-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 HNF 抗体包括 HNF-1, HNF-1 α, HNF-1 β, HNF-3 α, HNF-3 β, HNF-3 γ, HNF-4 α 和 HNF-6

HNF-3β抗体（RY-7）是一种IgG2a κ小鼠单克隆HNF-3β抗体（也称为HNF-3β抗体或FOXA2抗体），能够通过WB、IP、IF、IHC(P)和ELISA技术检测小鼠、大鼠和人类来源的HNF-3β。HNF-3β抗体（RY-7）以非偶联的抗HNF-3β抗体形式提供。HNF-1（α和β）、HNF-3（α、β和γ）、HNF-4（α和γ）以及HNF-6部分组成了一个被指定为肝细胞核因子家族的同蛋白家族。各种HNF-1异构体调节肝脏以及其他组织（如肾脏、小肠和胸腺）中的基因转录。HNF-3α、HNF-3β和HNF-3γ调节成年肝脏中众多肝细胞基因的转录。HNF-3α和HNF-3β还参与原肠胚形成事件，如体轴形成。HNF-4α和HNF-4γ对早期胚胎发育很重要。HNF-4α在肝脏、肾脏、胰腺、小肠、睾丸和结肠中表达；HNF-4γ在这些组织中除肝脏外均有表达。已显示HNF-6与HNF-3β的启动子结合，这表明HNF-6在肠内胚层上皮细胞分化中的潜在作用。有证据表明，HNF-6也可能是至少22个其他肝细胞富集基因的转录激活因子，包括细胞色素P450 2C13和α-1抗胰蛋白酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HNF-3β 抗体 (RY-7) 参考文献:

  1. 肝核因子-3（HNF-3 或 Foxa2）通过与 G1 和 G2 启动子元件结合来调节胰高血糖素基因的转录。  |  Gauthier, BR., et al. 2002. Mol Endocrinol. 16: 170-83. PMID: 11773447
  2. 腺病毒介导的 HNF-3beta 或 HNF-3alpha 的增加在肝糖原水平和基因表达方面显示出差异。  |  Tan, Y., et al. 2002. Hepatology. 35: 30-9. PMID: 11786957
  3. HNF-3beta是Jaagsiekte绵羊逆转录病毒长末端重复序列在II型肺细胞中表达的关键因素，而不是在克拉拉细胞中表达的关键因素。  |  McGee-Estrada, K., et al. 2002. Virology. 292: 87-97. PMID: 11878911
  4. 肝细胞中叉头盒 A2 (HNF-3beta) 转录因子水平升高会导致出生后脂肪变性和线粒体损伤。  |  Hughes, DE., et al. 2003. Hepatology. 37: 1414-24. PMID: 12774021
  5. 转录因子HNF-6/OC-1可抑制TGF-beta对小鼠肝脏中HNF-3α/Foxa1基因的刺激。  |  Plumb-Rudewiez, N., et al. 2004. Hepatology. 40: 1266-74. PMID: 15562441
  6. 通过替代 RNA 处理，可生成更强的转录激活剂或 HNF1 同源蛋白家族的显性抑制剂。  |  Bach, I. and Yaniv, M. 1993. EMBO J. 12: 4229-42. PMID: 7900999
  7. 小鼠转录因子 HNF-3 基因家族：基因结构, cDNA 序列和 mRNA 分布。  |  Kaestner, KH., et al. 1994. Genomics. 20: 377-85. PMID: 8034310
  8. 人类肝细胞核因子 4 同工型由不同的基因编码，且表达量不同。  |  Drewes, T., et al. 1996. Mol Cell Biol. 16: 925-31. PMID: 8622695
  9. 转录激活因子肝细胞核因子 6 可调节肝脏基因的表达。  |  Samadani, U. and Costa, RH. 1996. Mol Cell Biol. 16: 6273-84. PMID: 8887657

订购信息

产品名称产品编号规格价格数量收藏夹

HNF-3β 抗体 (RY-7)

sc-101060
100 µg/ml
RMB2504.00

HNF-3β (RY-7): m-IgG Fc BP-HRP 套装

sc-537298
100 µg Ab; 10 µg BP
RMB2662.00

HNF-3β (RY-7): m-IgGκ BP-HRP 套装

sc-534530
100 µg Ab; 40 µg BP
RMB2662.00

HNF-3β (RY-7): m-IgG2a BP-HRP 套装

sc-547968
100 µg Ab; 10 µg BP
RMB2662.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-10
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Rated 1 out of 5 by from Non-specific ImmunocytochemistryVery strong nuclear staining in undifferentiated hiPSCs. Immunocytochemistry 1:250 dilution.
Date published: 2025-01-20
Rated 4 out of 5 by from OK for IFgood nuclear staining after immunofluorescence on PFA-fixed cells at 1:1000 antibody dilution.
Date published: 2019-04-18
Rated 5 out of 5 by from Good for IF staining endoderm cellsI used this antibody in an IF protocol on hiPS cells as well as hiPS cells differentiated into endoderm cells. Staining in endoderm cells was much brighter, thus I would recommend the antibody to anyone looking to distinguish these two cell types from one another.
Date published: 2018-02-28
Rated 5 out of 5 by from Just a great productOutstanding western blot, strong signal, low background, also excellent IF and IHC results
Date published: 2017-01-06
Rated 5 out of 5 by from Band looks goodStrong, single band at target molecular weight by WB
Date published: 2016-12-23
Rated 4 out of 5 by from Positive staining of human stomach tissuePositive staining of human stomach tissue in IHC(P) -SCBT QC
Date published: 2015-05-13
Rated 4 out of 5 by from IF of Hep G2 cells shows nuclear localizationIF of Hep G2 cells shows nuclear localization of signal with PFA fixation -SCBT QC
Date published: 2015-03-09
Rated 4 out of 5 by from Good results in Western Blot with Hep G2Good results in Western Blot with Hep G2 and A549 nuclear extracts -SCBT QC
Date published: 2013-01-26
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HNF-3β 抗体 (RY-7) is rated 4.0 out of 5 by 8.
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