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HLA-A2 Antibody (BB7.2) - Flow Cytometry - Image 9497
HLA-A2 抗体 (BB7. 2) PE: sc-32236 PE. 人体外周血白细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG2b: sc-2868.
HLA-A2 Antibody (BB7.2) - Flow Cytometry - Image 9497
HLA-A2 抗体 (BB7. 2) PE: sc-32236 PE. 人体外周血白细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG2b: sc-2868.
HLA-A2 抗体 (BB7. 2) PE: sc-32236 PE. 人体外周血白细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG2b: sc-2868.

HLA-A2 抗体 (BB7.2): sc-32236

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寫評論提問

说明书
  • HLA-A2 抗体 BB7.2 是小鼠单克隆 IgG2b κ，HLA-A2抗体, 在12篇文献中引用，规格为200 µg/ml
  • 针对可溶性HLA-A2
  • HLA-A2 抗体 (BB7.2) 推荐用于FCM，检测human 来源的 epitope on α 2 domain of HLA-A2
  • HLA-A2 抗体 (BB7.2) 可偶联 藻红蛋白 或者 FITC 用于 FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HLA-A2 (BB7.2): sc-32236的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是HLA-A2 Antibody (BB7.2). 的首选辅助检测试剂。这些试剂现与HLA-A2 Antibody (BB7.2) 打包提供（请参阅下面的订购信息）。

快捷链接

描述
基因信息
说明书与实验方案
研究信息

関連項目

额外HLA抗体，包括HLA-A, HLA-A2, HLA-B, HLA-B27, HLA-B7, HLA-C, HLA class I, HLA-D, HLA-DM, HLA-DM α, HLA-DM β, HLA-DO β, HLA-DP, HLA-DP α 1, HLA-DP β 1和HLA-DQ

HLA-A2 抗体（BB7.2）是一种小鼠单克隆 IgG2b kappa 轻链抗体，可通过流式细胞术（FCM）检测人类来源的 HLA-A2 蛋白。抗HLA-A2抗体（BB7.2）有非结合型和多种结合型，包括藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可满足不同实验需求。人类白细胞抗原A2（HLA-A2）作为一类组织相容性分子，在免疫反应中发挥着至关重要的作用，它通过在细胞表面向细胞毒性T淋巴细胞（CTL）呈递肽，从而参与免疫反应。这一功能对于识别和消除感染或恶性细胞至关重要，使HLA-A2成为免疫监视的关键角色。值得注意的是，HLA-A2与接受干扰素α治疗慢性丙型肝炎患者的自身免疫性甲状腺功能障碍有关，这凸显了HLA-A2在免疫反应和疾病病理学中的重要性。此外，研究表明，HLA-A2抗原在HIV感染时下调，特别是在原代T细胞中，这会导致免疫应答的逃避。在包括β细胞和星形细胞瘤细胞在内的各种细胞系中也观察到了这种下调，表明对免疫逃避策略有更广泛的影响。HLA-A2重链的修饰可以增强HIV-1特异性表位的呈现，从而提高HLA-A2限制性CD8+ CTL对感染细胞的识别和裂解。抗HLA-A2抗体（BB7.2）是研究人员研究免疫反应复杂性的重要工具，特别是在病毒感染和自身免疫性疾病的情况下。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HLA-A2 抗体 (BB7.2) 参考文献:

  1. 艾滋病毒逃避细胞免疫反应。  |  Collins, KL. and Baltimore, D. 1999. Immunol Rev. 168: 65-74. PMID: 10399065
  2. [干扰素疗法诱发的自身免疫性甲状腺疾病]。  |  Murakami, M., et al. 1999. Nihon Rinsho. 57: 1779-83. PMID: 10483250
  3. 通过 HLA-A2 重链修饰创建 HIV-1 逆转录酶细胞毒性 T 淋巴细胞靶结构。  |  Dela Cruz, CS., et al. 2000. Int Immunol. 12: 1293-302. PMID: 10967024
  4. [人类肿瘤排斥抗原和肽从基因到临床研究]。  |  Itoh, K., et al. 2000. Nihon Geka Gakkai Zasshi. 101: 612-7. PMID: 11022676
  5. 增强低亲和力 HLA-A2.1 相关多肽免疫原性的一般策略：对识别隐性肿瘤表位的意义。1 相关多肽的免疫原性的一般策略：在鉴定隐性肿瘤表位方面的意义。  |  Tourdot, S., et al. 2000. Eur J Immunol. 30: 3411-21. PMID: 11093159
  6. HLA-A2 等位基因通过改变海马体积介导阿尔茨海默病  |  Wang, ZX., et al. 2017. Mol Neurobiol. 54: 2469-2476. PMID: 26979752
  7. 用于研究病毒特异性 CD8+ T 细胞的嵌合 HLA-A2:β2M:Ig 融合蛋白。  |  Ribeiro, ÁL., et al. 2021. J Immunol Methods. 492: 112997. PMID: 33600818
  8. 从食管鳞状细胞癌新抗原中鉴定 HLA-A2 受限突变表位  |  Wang, Z., et al. 2021. Vaccines (Basel). 9: PMID: 34696226
  9. 开发针对 NY-ESO-1/HLA-A2 的 TCR 类抗体和嵌合抗原受体，用于癌症免疫疗法。  |  Liu, X., et al. 2022. J Immunother Cancer. 10: PMID: 35338087
  10. 抗HLA-A2-CAR集落可延长血管化小鼠异位心脏异体移植的存活时间。  |  Wagner, JC., et al. 2022. Am J Transplant. 22: 2237-2245. PMID: 35434896
  11. 分子动力学模拟的无监督和有监督人工智能揭示了 HLA-A2 肽免疫原性的复杂特征。  |  Weber, JK., et al. 2023. Brief Bioinform. 25: PMID: 38233090
  12. 开发针对 DKK1 肽-HLA-A2 复合物的治疗性单克隆抗体，以治疗人类癌症。  |  Qian, J., et al. 2024. J Immunother Cancer. 12: PMID: 38267222

订购信息

产品名称产品编号规格价格数量收藏夹

HLA-A2 抗体 (BB7.2)

sc-32236
200 µg/ml
RMB2377.00

HLA-A2 (BB7.2): m-IgGκ BP-HRP 套装

sc-520823
200 µg Ab, 40 µg BP
RMB2662.00

HLA-A2 (BB7.2): m-IgG2b BP-HRP 套装

sc-548855
200 µg Ab; 10 µg BP
RMB2662.00

HLA-A2 抗体 (BB7.2) FITC

sc-32236 FITC
200 µg/ml
RMB2482.00

HLA-A2 抗体 (BB7.2) PE

sc-32236 PE
200 µg/ml
RMB2580.00

HLA-A2 抗体 (BB7.2) Alexa Fluor® 488

sc-32236 AF488
200 µg/ml
RMB2685.00

HLA-A2 抗体 (BB7.2) Alexa Fluor® 546

sc-32236 AF546
200 µg/ml
RMB2685.00

HLA-A2 抗体 (BB7.2) Alexa Fluor® 594

sc-32236 AF594
200 µg/ml
RMB2685.00

HLA-A2 抗体 (BB7.2) Alexa Fluor® 647

sc-32236 AF647
200 µg/ml
RMB2685.00

HLA-A2 抗体 (BB7.2) Alexa Fluor® 680

sc-32236 AF680
200 µg/ml
RMB2685.00

HLA-A2 抗体 (BB7.2) Alexa Fluor® 790

sc-32236 AF790
200 µg/ml
RMB2685.00

Does your antibody distinguish between specific subtypes of HLA-A2 such as HLA*02:01 and HLA*02:06?

Asked by: rmeaston
Thank you for your question. According to the following publication, this clone, BB7.2, has been shown to have polymorphic specificity. Please refer to the publication below for more detailed information. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615166/
Answered by: Technical Support
Date published: 2019-05-17

Is it suitable for western blot?

Asked by: vandana
Thank you for your question. No, this antibody is not recommended for Western blot.
Answered by: Technical Support
Date published: 2019-01-26

What negative control should be used when using the conjugated version of HLA-A2 (BB7.2): sc-32236 monoclonal antibody in flow cytometry?

Asked by: jenni
Thank you for your inquiry. We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-32236 PE, then you would want to use normal mouse IgG2b PE, sc-2868.
Answered by: Technical Support 15
Date published: 2017-01-12

Which secondary antibody do you recommend using with HLA-A2 (BB7.2): sc-32236?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with HLA-A2 (BB7.2): sc-32236 monoclonal antibody we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-12
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Rated 1 out of 5 by from Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and did not give a signal, even at dilution 1:10
Date published: 2018-11-19
Rated 5 out of 5 by from Strong fcm dataVery nice clear FCM results; would like to see additional applications
Date published: 2017-01-30
Rated 5 out of 5 by from Good for flowPublished antibody, good for flow cytometry for human HLA-A2 detection.
Date published: 2017-01-27
Rated 5 out of 5 by from Produced excellent FCM data for human peripheralProduced excellent FCM data for human peripheral blood leukocytes. -SCBT QC
Date published: 2014-11-13
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HLA-A2 抗体 (BB7.2) is rated 4.0 out of 5 by 4.
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