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Histone H3 抗体 (1G1): sc-517576
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寫評論提問- Histone H3 抗体 1G1 是小鼠单克隆 IgG, 在87篇文献中引用，提供 100 µg/ml
- 针对human来源的重组Histone H3蛋白片段
- 推荐用于 mouse, rat 和 human 来源的Histone H3 WB 和 IF检测
- 关于如何获取Histone H3 (1G1): sc-517576的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Histone H3 Antibody (1G1) for WB applications. 的首选辅助检测试剂，这些试剂现与Histone H3 Antibody (1G1) 打包提供（请参阅下面的订购信息）。
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基因信息
说明书与实验方案
研究信息
组蛋白H3抗体（1G1）是一种小鼠单克隆IgG抗体，可通过蛋白质印迹（WB）和免疫荧光（IF）检测小鼠、大鼠和人类样本中的组蛋白H3。抗组蛋白H3抗体（1G1）有非结合形式。组蛋白H3在染色质结构组织中起着至关重要的作用，对于DNA在细胞核中的包装至关重要。组蛋白H3是核小体八聚体结构的核心部分，它包裹着DNA，调节基因表达和DNA复制。组蛋白H3维持基因组的稳定性，促进转录和修复过程。组蛋白H3经历多种翻译后修饰，包括甲基化和乙酰化，影响DNA和蛋白质的相互作用，影响染色质结构和功能。这些修饰调节基因表达，影响细胞分化和环境反应。抗组蛋白H3抗体（1G1）的检测能力使其成为研究各种生物环境下染色质动态和表观遗传调控的宝贵试剂。
仅限研究使用。不适用于诊断和治疗用途。
| 种属 | 基因名称 | 基因ID | 染色体位置 | 亚型（mRNA）登录号 | 蛋白质登录号 | OMIM™ 号码 |
|---|---|---|---|---|---|---|
| Human | HIST1H3A | Entrez Gene ID: 8350 | 6p22.2 | NM_003529 | UniProt ID: P68431 | 602810 |
| Mouse | Hist1h3a | Entrez Gene ID: 360198 | 13 A3.1 | NM_013550 | UniProt ID: P68433 | N/A |
针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz®偶联抗体，从而可以省去使用二抗。
圣克鲁斯生物技术的抗体有超过36万的研究引文。抗Histone H3抗体(1G1)在各种科学出版物中有87篇引文。
针对哺乳动物靶标蛋白的一抗，如抗Histone H3 抗体 (1G1)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。 许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。
Histone H3 抗体 (1G1) 规格
替代产品名称: Histone H3
产品克隆名称: 1G1
分类: 一抗
Clonality: 单克隆抗体
抗体同型: IgG
氨基酸:
抗原表位靶标: Histone H3
抗原表位种属: human
产品反应性/检测: Histone H3
种属反应性/检测: mouse, rat, human
其它哺乳动物反应性: N/A
其它非哺乳动物反应性: N/A
应用: WB, IF
瓶的信息: Each vial contains 100 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and 1% glycerol
研究引文: 87篇各种科学出版物的引文
Histone H3 抗体 (1G1)可选的偶联物/形式: N/A
针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.
圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗Histone H3抗体(1G1)推荐用于以下应用：WB (Western blotting), IF (Immunofluorescence).
我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。
组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。
单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。
偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。
正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。
優選的 Ac-Histone H3 抗體是 Ac-Histone H3 抗體 (AH3-120): sc-56616.
订购信息
| 产品名称 | 产品编号 | 规格 | 价格 | 数量 | 收藏夹 | |
Histone H3 抗体 (1G1) | sc-517576 | 100 µg/ml | RMB2377.00 | |||
Histone H3 (1G1): m-IgG Fc BP-HRP 套装 | sc-541115 | 100 µg Ab; 10 µg BP | RMB2662.00 | |||
Histone H3 (1G1): m-IgG1 BP-HRP 套装 | sc-542591 | 100 µg Ab; 20 µg BP | RMB2662.00 |
I performed ICC with this antibody in mitotic cells, but I couldn't detect histone H3 signal co-localized with the DAPI signal. Could you help me solve this issue?
Asked by: KWJ94
Thank you for your product review. We are sorry to hear that this antibody did not perform for you as expected. There are many possible causes for no signal. Please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website, and we would be happy to help you figure this out. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase and will do our best to help you achieve the best results possible!
Answered by: Jiajun Z
Date published: 2024-09-29
Hi can we please get a sample of sc-517576? Do you have that available? We just purchased sc-390935 with Texas A&M University Biology Department, Dr. Deb Bell-Pedersen lab.
Asked by: Johnny
Thank you for contacting Santa Cruz Biotechnology. To request a sample please email [email protected]
Answered by: BlakeJ
Date published: 2024-06-18
Just received a free sample, which dilution would you recommend for nuclear extracts WB?
Asked by: IngOlmos
Thank you for your question. Dilution recommendations can be found in the product's datasheet, which is available right here on the product page. For western blots we recommend a starting dilution of 1:200, and a dilution range between 1:100-1:1000. When working with nuclear extracts, an amount of protein between 10–20 µg is appropriate.
Answered by: Tech Support Europe
Date published: 2023-05-15
could it be possible to buy directly bound to alexa488 or 594 please
Asked by: antonio blesa
Thank you for your question. If you are interested in this antibody directly conjugated with AF488 or AF594, please contact our our Technical Service. We should be able to provide this format as special order.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-31
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Rated 4 out of
5 by
LabProteomica from
Good work!!This antibody gave a strong and clean signal when we used it at a 1:100 diluition with 80μg of protein samples.
Date published: 2023-02-17
Rated 5 out of
5 by
0015 from
H3 Ab for Western BlottingAsked for a sample before purchasing. Worked straight away on CRC cell lines HCT116 and DLD1 (drug treated and untreated) when loading 20 ug of whole protein lysates and using 1:500 dilution in 5% BSA/TBST. Would definitely recommend.
Date published: 2023-01-24
Rated 5 out of
5 by
Xiuju Li from
western blotI used this antibody for my mouse primary neuron cells western blot. I got very nice and clean bolt!
Date published: 2022-08-24
Rated 5 out of
5 by
cvvfr from
good and strongThe antibody was tested on whole lysate from liver cancer cell lines (JHH6 andHuh7) and immortalized hepatocytes (IHH).
Antibody: sensitive and specific. Too strong signal with the dilution you recommend. Conditions need to be better set.
Apparent MW: about 15kDa.
Expected MW: 15kDa.
• 40ug/lane cell lysate
• Transfer 1h30 100V, PVDF (0.2 µm)
• Blocking milk 4% in 0.1% Tween -PBS 1h RT
• Anti-HISTONE H3 (1G1)(sc-517576) 1:200 O/N 4°C in blocking solution
• Anti-mouse HRP 1:1000 1h RT in blocking solution
• Developed with ECL Plus
Date published: 2022-06-09
Rated 5 out of
5 by
Kaze from
Very good!In MB231 cells, a clear band appeared after WB analysis 1: 2000 dilution.
Date published: 2022-03-18
Rated 4 out of
5 by
Anonymous from
Useful for my purposesI asked it as free trial and i worked well enough, so I think I can use it for my purposes.
Date published: 2020-12-23
Rated 5 out of
5 by
DennisF from
Great for IFBought this antibody recently for the purpose of immunofluorescence on human neuroblastoma cell lines. It worked very well.
Date published: 2019-09-03
Rated 5 out of
5 by
xuenv32 from
Works very well with 1:5000 dilutionWorks very well with 1:5000 dilution, and I hope some more conjugation products can be developed with this antibody.
Date published: 2019-02-05
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Histone H3 抗体 (1G1) is rated
4.7 out of
5 by
10.
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版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。