Date published: 2025-9-7
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Histone H2B Antibody (A-6) - Western Blotting - Image 380180
组蛋白 H2B 抗体 (A-6): sc-515808. MCF7 (A), NAMALWA (B) 和 PC-12 (F) 全细胞裂解液以及 WEHI-231 (C), NIH/3T3 (D) 和 MOLT-4 (E) 核提取物中组蛋白 H2B 表达的 Western 印迹分析.
组蛋白 H2B 抗体 (A-6): sc-515808. MCF7 (A), NAMALWA (B) 和 PC-12 (F) 全细胞裂解液以及 WEHI-231 (C), NIH/3T3 (D) 和 MOLT-4 (E) 核提取物中组蛋白 H2B 表达的 Western 印迹分析.

Histone H2B 抗体 (A-6): sc-515808

4.0(6)
寫評論提問

说明书
  • Histone H2B抗体(A-6)是小鼠单克隆IgM κ, 在17篇文献中引用，规格为200 µg/ml
  • 免疫human物种的的氨基酸1-126
  • 推荐用于 mouse, rat 和 human 来源的Histone H2B WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取Histone H2B (A-6): sc-515808的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 Histone H2B 抗体 (A-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Histone H2B 抗体 (A-6) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
说明书与实验方案
研究信息

関連項目

此外 Histone 抗体 包括 Histone cluster 1 H1, Histone H2A, Histone H3, Histone H4和Histone cluster 1 H3A

组蛋白H2B抗体（A-6）是一种小鼠单克隆IgM抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的组蛋白H2B。抗组蛋白H2B抗体（A-6）以非结合形式提供，可用于多种实验。组蛋白H2B在染色质结构组织中起着至关重要的作用，因为组蛋白H2B是核小体（nucleosome）的核心组成部分之一，而核小体对于DNA在细胞核内的包装至关重要。核小体结构由两个H2A-H2B二聚体和两个H3-H4二聚体组成的八聚体构成，可将DNA压缩成更易于处理的形式，从而促进转录、复制和修复等过程。组蛋白H2B对于维持基因组稳定性和调节基因表达至关重要，因为组蛋白组成或修饰的改变可能会导致严重的细胞后果，包括各种疾病的发生。此外，组蛋白H2B会经历许多翻译后修饰，例如乙酰化和甲基化，这些修饰会影响DNA相互作用和其他核蛋白，从而影响染色质动态和细胞功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Histone H2B 参考文献:

  1. 蛋白质图案会读取组蛋白密码吗？  |  de la Cruz, X., et al. 2005. Bioessays. 27: 164-75. PMID: 15666348
  2. 染色质中组蛋白 H1 功能的动态变化。  |  Bustin, M., et al. 2005. Mol Cell. 17: 617-20. PMID: 15749012
  3. 组蛋白 H3 的诱导性共价翻译后修饰。  |  Bode, AM. and Dong, Z. 2005. Sci STKE. 2005: re4. PMID: 15855410
  4. 组蛋白合成和核小体组装的调控。  |  Gunjan, A., et al. 2005. Biochimie. 87: 625-35. PMID: 15989979
  5. 组蛋白赖氨酸甲基化的多种功能。  |  Martin, C. and Zhang, Y. 2005. Nat Rev Mol Cell Biol. 6: 838-49. PMID: 16261189
  6. 组蛋白 H1 的基因调控：与 DNA 甲基化的新联系。  |  Rupp, RA. and Becker, PB. 2005. Cell. 123: 1178-9. PMID: 16377556
  7. 组蛋白翻译后修饰和对 DNA 双链断裂的反应  |  Wurtele, H. and Verreault, A. 2006. Curr Opin Cell Biol. 18: 137-44. PMID: 16487697
  8. 组蛋白修饰：信号受体和遗传表观遗传密码的潜在要素。  |  Nightingale, KP., et al. 2006. Curr Opin Genet Dev. 16: 125-36. PMID: 16503131
  9. 组蛋白 H3 变体及其在哺乳动物基因组索引中的潜在作用：'H3 条形码假说'。  |  Hake, SB. and Allis, CD. 2006. Proc Natl Acad Sci U S A. 103: 6428-35. PMID: 16571659
  10. 低二碳酸血症诱导的 microRNA449b 上调下调 Nrf-1 会加重糖尿病小鼠的心脏缺血再灌注损伤。  |  Meng, Z., et al. 2023. J Mol Cell Cardiol. 182: 1-14. PMID: 37437402
  11. hnRNPH1 在线粒体应激状态下通过建立 NRF1/DRP1 逆向信号维持线粒体稳态。  |  Zhao, L., et al. 2025. Cell Death Differ. 32: 118-133. PMID: 38898233
  12. 肝细胞应激防御因子 Nrf1 和 Nrf2 对 MASLD 进展的保护作用  |  Akl, MG., et al. 2024. Int J Mol Sci. 25: PMID: 39125617

订购信息

产品名称产品编号规格价格数量收藏夹

Histone H2B 抗体 (A-6)

sc-515808
200 µg/ml
RMB2377.00

Histone H2B (A-6): m-IgGκ BP-HRP 套装

sc-525229
200 µg Ab, 40 µg BP
RMB2662.00

We have used antibody H2B  (N-20): sc-8650 for a long time, both for IF and for western. It did work, but not perfectly. I see today you sale H2B (A-6): sc-515808 instead of sc-8650. How can  I ask for a 10ug sample. Can you give me a Tel number in China

Asked by: Liyongzheng
Thank you for your question. For a limited time, we are offering a free 10 µg sample of our highly recommended mouse monoclonal replacement, Histone H2B Antibody (A-6): sc-515808. Please contact our Technical Service Department to request a sample. You can reach our Asian Support office toll-free at 800-820-8626, e-mail [email protected], or contact us by live chat. The direct telephone number for our office in Shanghai, China is (86 21) 6093-6350.
Answered by: Technical Support
Date published: 2017-03-11

How much sample do you recommend loading on the gel for Western blot detection using Histone H2B (A-6): sc-515808?

Asked by: Germaine
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from Not workingI bought this antibody recently and its not working at all. I've tried at several dilution (for e.g. 1:250 to 1:1000) with 50ug of total protein (HeLa cells). Please suggest me regarding this. Thank you
Date published: 2022-06-05
Rated 5 out of 5 by from Nic antibodyThis antibody can work well even in low concentration.
Date published: 2018-08-01
Rated 5 out of 5 by from Excellent antibodyGreat antibody. Works for both Western Blot (1:1000 dilution) and IP. Will purchase again.
Date published: 2018-04-27
Rated 4 out of 5 by from Great clear bandsI tried it and got my bands. I used cell lysates from cell lines.
Date published: 2017-10-23
Rated 4 out of 5 by from Histone H2B antibody (A-6): sc-515808Work well in western blot at 1:200, human H2B. And when we use the Penicillium.oxalicum H2B, we don’t detect the H2B.
Date published: 2017-08-18
Rated 5 out of 5 by from Great for westernI got a strong band around 18 kDa in mouse whole tissue lysate and chromatin fraction but not in cytoplasmic samples.
Date published: 2017-08-14
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Histone H2B 抗体 (A-6) is rated 4.0 out of 5 by 6.
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