Date published: 2025-9-7

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HIF PHD2 抗体 (H-8): sc-271835

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说明书
  • HIF PHD2 抗体 H-8 是小鼠单克隆 IgG1 κ,HIF PHD2抗体, 在20篇文献中引用,规格为200 µg/ml
  • 针对human起源的HIF PHD2内部区域内的氨基酸240-288进行
  • 推荐用于 mouse, rat 和 human 来源的HIF PHD2 WB, IP, IF 和 ELISA检测
  • 抗HIF PHD2抗体(H-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HIF PHD2 (H-8): sc-271835免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是HIF PHD2 Antibody (H-8) for WB applications. 的首选辅助检测试剂,这些试剂现与HIF PHD2 Antibody (H-8) 打包提供(请参阅下面的订购信息)。

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関連項目

HIF PHD2抗体(H-8)是一种IgG1 κ小鼠单克隆HIF PHD2抗体(也称为EGLN1抗体、Egl-9家族缺氧诱导因子1抗体、PHD2抗体、脯氨酸羟化酶结构域包含蛋白2抗体、缺氧诱导因子脯氨酸羟化酶2抗体、HIF-脯氨酸羟化酶2抗体、Egl Nine同源物1抗体、C1orf12抗体、HIF-PH2抗体、HIFPH2抗体、ZMYND6抗体、HPH-2抗体、SM-20抗体或锌指MYND结构域包含蛋白6抗体),该抗体可通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附试验检测小鼠、大鼠和人类来源的HIF PHD2蛋白。HIF PHD2抗体(H-8)既有非偶联抗HIF PHD2抗体形式,也有多种偶联形式的抗HIF PHD2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。脯氨酸羟化酶结构域蛋白HIF PHD1、HIF PHD2和HIF PHD3(在啮齿动物中分别称为PHD1、PHD2和PHD3)能够使HIF-α亚基羟基化。缺氧诱导因子(HIF)是一种转录调节因子,在氧稳态的多个方面都具有重要作用。脯氨酸羟化酶催化HIF-α蛋白中4-羟脯氨酸的翻译后形成。HIF PHD1广泛表达,在睾丸中的表达水平最高,作为细胞氧传感器在细胞生长调节中具有重要作用。HIF PHD1可以定位于细胞核或细胞质,也可在激素反应组织中检测到,如正常和癌变的乳腺、卵巢和前列腺上皮。HIF PHD1由EGLN2编码,EGLN2位于染色体19q13.3上。HIF PHD2被视为主要的细胞氧传感器,因为针对HIF PHD2的RNA干扰,而不是针对HIF PHD1或HIF PHD3的RNA干扰,足以在常氧条件下稳定HIF-1α。HIF PHD2作为直接的HIF靶基因,主要在骨骼肌、心脏、肾脏和大脑中表达。HIF PHD3可能在调节肌细胞生长和神经细胞组织凋亡中发挥作用。HIF PHD3广泛表达,尽管在胎盘和心脏中可以检测到最高水平。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HIF PHD2 抗体 (H-8) 参考文献:

  1. 缺氧诱导因子-1(HIF-1)通过诱导 HIF-α-prolyl-4-hydroxylases 促进其降解。  |  Marxsen, JH., et al. 2004. Biochem J. 381: 761-7. PMID: 15104534
  2. 缺氧诱导因子对 HIF 脯氨酰羟化酶的调控。  |  Aprelikova, O., et al. 2004. J Cell Biochem. 92: 491-501. PMID: 15156561
  3. 脯氨酰羟化酶 PHD1, PHD2 和 PHD3 在调节缺氧诱导因子中的不同功能。  |  Appelhoff, RJ., et al. 2004. J Biol Chem. 279: 38458-65. PMID: 15247232
  4. 脯氨酰羟化酶结构域蛋白 2(phd2/egln-1)基因的调控:功能性缺氧反应元件的鉴定。  |  Metzen, E., et al. 2005. Biochem J. 387: 711-7. PMID: 15563275
  5. HIF-1-PHD2轴控制髓核细胞中syndecan 4的表达。  |  Fujita, N., et al. 2014. FASEB J. 28: 2455-65. PMID: 24558194
  6. I 类和 IIa 类 HDAC 通过 PHD2 依赖性机制介导 HIF-1α 的稳定性,而 IIb 类成员 HDAC6 则促进椎间盘核髓细胞中 HIF-1α 的转录活性。  |  Schoepflin, ZR., et al. 2016. J Bone Miner Res. 31: 1287-99. PMID: 26765925
  7. 肝脏PHD2/HIF-1α轴参与运动后全身能量平衡。  |  Luo, B., et al. 2018. FASEB J. 32: 4670-4680. PMID: 29601782
  8. LRP5 通过与缺血心肌中的 PHD2 相互作用调节 HIF-1α 的稳定性  |  Ju, S., et al. 2021. Int J Mol Sci. 22: PMID: 34205318
  9. 脂肪来源间充质干细胞通过PHD2/HIF-1α通路在结肠炎小鼠中重新编程M1巨噬细胞代谢。  |  Yuan, Y., et al. 2022. Front Immunol. 13: 859806. PMID: 35757749
  10. 脂肪细胞衍生的乳酸是一种信号代谢产物,可通过靶向PHD2来增强脂肪巨噬细胞的炎症。  |  Feng, T., et al. 2022. Nat Commun. 13: 5208. PMID: 36064857
  11. ETV2调节PHD2-HIF-1α轴控制代谢重编程,促进血管化骨再生。  |  Du, H., et al. 2024. Bioact Mater. 37: 222-238. PMID: 38549772
  12. 阿拉洛苷A通过巨噬细胞中的PHD2/HIF-1α减轻败血症引起的急性肺损伤。  |  Ma, J., et al. 2024. Phytomedicine. 135: 156089. PMID: 39366158

订购信息

产品名称产品编号规格价格数量收藏夹

HIF PHD2 抗体 (H-8)

sc-271835
200 µg/ml
RMB2377.00

HIF PHD2 (H-8): m-IgG Fc BP-HRP 套装

sc-527297
200 µg Ab; 10 µg BP
RMB2662.00

HIF PHD2 (H-8): m-IgG1 BP-HRP 套装

sc-532670
200 µg Ab; 20 µg BP
RMB2662.00

HIF PHD2 抗体 (H-8) AC

sc-271835 AC
500 µg/ml, 25% agarose
RMB3129.00

HIF PHD2 抗体 (H-8) HRP

sc-271835 HRP
200 µg/ml
RMB2377.00

HIF PHD2 抗体 (H-8) FITC

sc-271835 FITC
200 µg/ml
RMB2482.00

HIF PHD2 抗体 (H-8) PE

sc-271835 PE
200 µg/ml
RMB2580.00

HIF PHD2 抗体 (H-8) Alexa Fluor® 488

sc-271835 AF488
200 µg/ml
RMB2685.00

HIF PHD2 抗体 (H-8) Alexa Fluor® 546

sc-271835 AF546
200 µg/ml
RMB2685.00

HIF PHD2 抗体 (H-8) Alexa Fluor® 594

sc-271835 AF594
200 µg/ml
RMB2685.00

HIF PHD2 抗体 (H-8) Alexa Fluor® 647

sc-271835 AF647
200 µg/ml
RMB2685.00

HIF PHD2 抗体 (H-8) Alexa Fluor® 680

sc-271835 AF680
200 µg/ml
RMB2685.00

HIF PHD2 抗体 (H-8) Alexa Fluor® 790

sc-271835 AF790
200 µg/ml
RMB2685.00

对小鼠组织可以染色么?

Asked by: Meng Li
感谢您的提问。 HIF PHD2 Antibody (H-8):sc-271835可以通过免疫荧光方法检测小鼠样本PHD2。 Thank you for your inquiry. HIF PHD2 Antibody (H-8):sc-271835 is recommended for detection of PHD2 of mouse origin by IF.
Answered by: Technical Support 9
Date published: 2021-07-09

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-271835: HIF PHD2 (H-8) mouse monoclonal antibody will work?

Asked by: jenniferc
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 1 out of 5 by from No signal at allNo signal at all for WB in human lung fibroblasts.
Date published: 2017-12-20
Rated 4 out of 5 by from HIF PHD2我使用的是该抗体的小样试用装,以1:500稀释用作Western Blot实验,首次孵育时条带较清晰,第二第三次再使用时效果没那么好
Date published: 2017-11-23
Rated 5 out of 5 by from The antibody is really greatI was very experienced with the WB experimen, use HIF PHD2 antibody can see the specific bands in 46kDa.
Date published: 2017-10-25
Rated 5 out of 5 by from Work well in WB, human MSCsClear band can be detected by Western blot at 1:200 in human MSCs.
Date published: 2017-09-18
Rated 5 out of 5 by from 研究HIF1用来研究HIF1a的,试试 效果,后面准备染色试试,HIF1本身不好爆出来,这个应该还行,santa抗体染色一直都是不错的
Date published: 2017-05-04
Rated 5 out of 5 by from Nice featuresWe test this antibody on c2c12 cells and turbot cells in western blotting respectively, the first one is better than the other, though there is nonspecific bands.
Date published: 2017-03-17
Rated 5 out of 5 by from Immunoblotting with this antibodyI used this antibody to perform Western blotting and found that the product has good sensitivity and specificity, and will continue to be used in the future.
Date published: 2017-02-08
Rated 5 out of 5 by from The Best AntibodyMethods The cellular localization of HIF PHD2 antibody in mouse kidney tissue was detected by immunoblot and immunohistochemistry. We feel good about the antibody.
Date published: 2017-02-07
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HIF PHD2 抗体 (H-8) is rated 4.6 out of 5 by 13.
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