Date published: 2025-9-11

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Hep B cAg 抗体 (10E11): sc-23947

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说明书
  • Hep B cAg抗体(10E11)是小鼠单克隆IgG1 κ, 在20篇文献中引用,规格为200 µg/ml
  • 与变性重组HBcAg反应
  • 抗-Hep B cAg 抗体 (10E11) 推荐用于 WB, IP, IF, FCM 和 ELISA,检测Hep B 和Woodchuck hepatitis 来源的 an epitope corresponding to amino acids 8-20 of denatured core antigen
  • 抗Hep B cAg抗体(10E11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Hep B cAg (10E11): sc-23947免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Hep B cAg Antibody (10E11) for WB applications. 的首选辅助检测试剂,这些试剂现与Hep B cAg Antibody (10E11) 打包提供(请参阅下面的订购信息)。

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関連項目

Hep B cAg抗体(10E11)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、流式细胞术(FCM)和酶联免疫吸附测定(ELISA)检测乙型肝炎和土拨鼠肝炎中的Hep B cAg。抗乙肝核心抗原抗体(10E11)识别与乙肝病毒变性核心抗原的氨基酸8-20相对应的表位,这对于了解病毒生命周期和发病机理至关重要。核心抗原在病毒衣壳组装、包裹病毒基因组以及确保复制过程中的稳定性方面发挥着至关重要的作用。这种结构完整性对于病毒感染宿主细胞和逃避免疫反应至关重要,因此核心抗原成为诊断和治疗策略的关键目标。Hep B cAg 单克隆抗体(10E11)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 结合物,可满足不同实验需求。乙型肝炎病毒属于肝病毒科,可导致严重的肝脏炎症、黄疸,甚至可能致命,这凸显了在临床环境中准确检测和监测抗原的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Hep B cAg 抗体 (10E11) 参考文献:

  1. 携带前S1表位的重组乙型肝炎核心抗原可诱导针对慢性乙型肝炎病毒感染的免疫反应。  |  Chen, X., et al. 2004. Vaccine. 22: 439-46. PMID: 14670326
  2. 乙型肝炎核心抗原抗体与丙型肝炎相关肝细胞癌风险:一项前瞻性研究。  |  Ikeda, K., et al. 2007. Ann Intern Med. 146: 649-56. PMID: 17470833
  3. 乙型肝炎核心抗原与先天免疫系统的相互作用。  |  Lee, BO., et al. 2009. J Immunol. 182: 6670-81. PMID: 19454661
  4. 接受抗肿瘤坏死因子治疗的类风湿性肝炎核心抗原(HBV隐性携带者)患者体内乙型肝炎病毒(HBV)重新激活。  |  Lee, YH., et al. 2013. Clin Exp Rheumatol. 31: 118-21. PMID: 23111095
  5. HBsAg阴性健康献血者中乙型肝炎核心抗原抗体和乙型肝炎病毒DNA的流行率。  |  Karimi, G., et al. 2016. Virol J. 13: 36. PMID: 26944046
  6. 乙型肝炎病毒核心抗原突变可预测原发性肝细胞癌患者的术后预后。  |  Jia, J., et al. 2017. J Gen Virol. 98: 1399-1409. PMID: 28640739
  7. 乙型肝炎核心抗原通过TLR2通路抑制M2巨噬细胞的极化,同时促进其炎症细胞因子的产生。  |  Yi, H., et al. 2020. Front Immunol. 11: 535. PMID: 32292408
  8. 乙肝核心抗原调节外泌体miR-135a靶向囊泡相关膜蛋白2,促进肝细胞癌的耐药性。  |  Wei, XC., et al. 2021. World J Gastroenterol. 27: 8302-8322. PMID: 35068871
  9. 自噬在泛素化乙型肝炎病毒核心抗原增强树突状细胞功能中的作用。  |  Huang, R., et al. 2022. Viral Immunol. 35: 629-639. PMID: 36315216
  10. GE11-抗原负载的乙肝病毒核心抗原病毒样颗粒能够有效结合TNBC肿瘤。  |  Zhang, L., et al. 2023. Front Oncol. 13: 1110751. PMID: 37020877

订购信息

产品名称产品编号规格价格数量收藏夹

Hep B cAg 抗体 (10E11)

sc-23947
200 µg/ml
RMB2377.00

Hep B cAg (10E11): m-IgG Fc BP-HRP 套装

sc-526638
200 µg Ab; 10 µg BP
RMB2662.00

Hep B cAg (10E11): m-IgG1 BP-HRP 套装

sc-532011
200 µg Ab; 20 µg BP
RMB2662.00

Hep B cAg 抗体 (10E11) AC

sc-23947 AC
500 µg/ml, 25% agarose
RMB3129.00

Hep B cAg 抗体 (10E11) HRP

sc-23947 HRP
200 µg/ml
RMB2377.00

Hep B cAg 抗体 (10E11) FITC

sc-23947 FITC
200 µg/ml
RMB2482.00

Hep B cAg 抗体 (10E11) PE

sc-23947 PE
200 µg/ml
RMB2580.00

Hep B cAg 抗体 (10E11) Alexa Fluor® 488

sc-23947 AF488
200 µg/ml
RMB2685.00

Hep B cAg 抗体 (10E11) Alexa Fluor® 546

sc-23947 AF546
200 µg/ml
RMB2685.00

Hep B cAg 抗体 (10E11) Alexa Fluor® 594

sc-23947 AF594
200 µg/ml
RMB2685.00

Hep B cAg 抗体 (10E11) Alexa Fluor® 647

sc-23947 AF647
200 µg/ml
RMB2685.00

Hep B cAg 抗体 (10E11) Alexa Fluor® 680

sc-23947 AF680
200 µg/ml
RMB2685.00

Hep B cAg 抗体 (10E11) Alexa Fluor® 790

sc-23947 AF790
200 µg/ml
RMB2685.00

What is the recommended secondary for this mouse monoclonal antibody? When HBcAg was overexpressed, the goat anti-mouse IgG(H+L) Alexa Fluor488 or 647 of invitrogen and biolegend can not be used for the detection by IF or FCM.

Asked by: Anonymous
Thank you for your question. m-IgG1 BP-CFL 488: sc-533661 is the recommended secondary antibody for IF and FCM.
Answered by: Technical Service SH
Date published: 2023-11-08

What is the recommended secondary for this mouse monoclonal antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service
Date published: 2017-01-27
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Rated 5 out of 5 by from Great Clean Staining (IHC-P)Tested on multiple cases of human Hepatitis B infected liver tissue. The antibody is very sensitive and gives clean strong nuclear staining of infected hepatocytes. Performed on FFPE tissue at 1:200 dilution on Leica Bond III Immunostainer with Bond Polymer Refine assay and 20 mins retrieval in ER2.
Date published: 2019-12-12
Rated 5 out of 5 by from Produced publishable WB data on Huh7 cellsSeveral references in the primary literature for WB showing strong bands.
Date published: 2016-12-23
Rated 3 out of 5 by from CleanClean, crisp band seen in WB using human recombinant HBcAg. -SCBT QC
Date published: 2014-09-17
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Hep B cAg 抗体 (10E11) is rated 4.3 out of 5 by 3.
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