Date published: 2025-9-8
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CD44 Antibody (IM7) - Western Blotting - Image 138553
HCAM 抗体 (IM7): sc-18849. HUV-EC-C (A), 人 PBL (B), HeLa (C) 和 WI 38 (D) 全细胞裂解液中 HCAM 表达的 Western 印迹分析.
HCAM 抗体 (IM7): sc-18849. HUV-EC-C (A), 人 PBL (B), HeLa (C) 和 WI 38 (D) 全细胞裂解液中 HCAM 表达的 Western 印迹分析.

CD44 抗体 (IM7): sc-18849

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说明书
  • CD44 抗体 IM7 是大鼠单克隆 IgG2b, 在48篇文献中引用，提供 200 µg/ml
  • 针对SJL mouse自发骨髓性白血病M1的Dexamethasone诱导细胞
  • 抗-CD44 抗体 (IM7) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat, human, canine, feline 和equine 来源的 HCAM
  • 抗CD44抗体（IM7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD44 (IM7): sc-18849的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CD44 抗体 (IM7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

HCAM抗体（IM7）是一种鼠单克隆IgG2b抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）等方法检测小鼠、大鼠、人类、犬、猫和马样本中的HCAM。抗HCAM抗体（IM7）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。HCAM，也称为CD44，是一种742个氨基酸的单通道I型膜蛋白，在细胞粘附和迁移中起着至关重要的作用，它充当透明质酸（HA）的受体，并与骨桥蛋白（OPN）等配体相互作用。这种相互作用介导细胞与细胞以及细胞与基质的连接，在造血、淋巴细胞活化和肿瘤转移等过程中至关重要。HCAM的结构包括一个链接域，并经历可变剪接，产生多种同种型，包括CD44R、CDw44、CD44S、CD44H（造血）和CD44E（上皮）。CD44H同种型在癌组织中表达量高，表明其在肿瘤进展中起着重要作用，并凸显了HCAM在正常生理过程和病理条件下的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD44 抗体 (IM7)

sc-18849
200 µg/ml
RMB2377.00

CD44 抗体 (IM7) AC

sc-18849 AC
500 µg/ml, 25% agarose
RMB3129.00

CD44 抗体 (IM7) HRP

sc-18849 HRP
200 µg/ml
RMB2377.00

CD44 抗体 (IM7) FITC

sc-18849 FITC
200 µg/ml
RMB2482.00

CD44 抗体 (IM7) PE

sc-18849 PE
200 µg/ml
RMB2580.00

CD44 抗体 (IM7) Alexa Fluor® 488

sc-18849 AF488
200 µg/ml
RMB2685.00

CD44 抗体 (IM7) Alexa Fluor® 546

sc-18849 AF546
200 µg/ml
RMB2685.00

CD44 抗体 (IM7) Alexa Fluor® 594

sc-18849 AF594
200 µg/ml
RMB2685.00

CD44 抗体 (IM7) Alexa Fluor® 647

sc-18849 AF647
200 µg/ml
RMB2685.00

CD44 抗体 (IM7) Alexa Fluor® 680

sc-18849 AF680
200 µg/ml
RMB2685.00

CD44 抗体 (IM7) Alexa Fluor® 790

sc-18849 AF790
200 µg/ml
RMB2685.00

Hi there, I have two question regarding the antibody- 1. which specific epitope of the whole CD44 protein does the antibody bind? 2. how good it is compared to other CD44 antibody in your website- it is HCAM Antibody (DF1485): sc-7297?

Asked by: anusi
Thank you for your question. Please contact technical service for information regarding the antibody epitope and data.
Answered by: Tech Service
Date published: 2021-06-15

Salve, Scrivo dal dipartimento di medicina sperimentale e clinica dell'Università di Firenze. Sono interessato a provare i 10ug di campione gratuito dell'Anticorpo HCAM (IM7): sc-18849. E' disponibile coniugato con PerCP? In caso come devo procedere?

Asked by: Cesare
Molte grazie per la sua domanda. La possibilità di provare i nostri anticorpi é limitata ai primari non coniugati. Consideri che offriamo un anno di garanzia dalla data di consegna per l´applicazione/specie raccomandata. Contatti il nostro servizio tecnico tramite telefono, email o live chat per ricevere ulteriori informazioni.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

If I want to use this antibody for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: TinTin
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support 2
Date published: 2017-01-18
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Rated 5 out of 5 by from Ottimo funzionamentoHo provato il trial e sono molto soddisdatta. PC3, DU145, 22Rv1, HCT15 e HCT116
Date published: 2024-12-09
Rated 5 out of 5 by from 功能很出色我一个月前购买了这个商品，觉得很好！这次想拿这个试用装再尝试一下，希望能通过！十分感谢，愿大家一直实验成功！
Date published: 2022-08-09
Rated 5 out of 5 by from Wow !! OutstandingOutstanding western,IF and IHC ; strong signal,low background
Date published: 2017-01-06
Rated 5 out of 5 by from VersatileExcellent HCAM option for Flow, Western Blot, and IHC(P).
Date published: 2016-12-23
Rated 5 out of 5 by from Produced nice FCM data for mouse peripheralProduced nice FCM data for mouse peripheral blood leukocytes. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Published immunoperoxidase staining dataPublished immunoperoxidase staining data obtained using paraffin embedded white adipose tissue (WAT). -SCBT Publication Review
Date published: 2015-03-18
Rated 5 out of 5 by from Used for verification of HCAM knockdown inUsed for verification of HCAM knockdown in gubernaculum cells (WB). -SCBT Publication Review
Date published: 2015-02-21
Rated 5 out of 5 by from Used at 1:100 dilution for IF studies usingUsed at 1:100 dilution for IF studies using murine knee joints. -SCBT Publication Review
Date published: 2015-02-05
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CD44 抗体 (IM7) is rated 5.0 out of 5 by 10.
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