Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
CD44 Antibody (DF1485) - Western Blotting - Image 352076
HCAM 抗体 (DF1485): sc-7297. U-937 (A), CCRF-CEM (B) 和 NCI-H1299 (C) 全细胞裂解液中 HCAM 表达的 Western 印迹分析.
HCAM 抗体 (DF1485): sc-7297. U-937 (A), CCRF-CEM (B) 和 NCI-H1299 (C) 全细胞裂解液中 HCAM 表达的 Western 印迹分析.

CD44 抗体 (DF1485): sc-7297

4.5(19)
寫評論提問

说明书
  • CD44 抗体 DF1485 是小鼠单克隆 IgG1 κ，CD44抗体, 在164篇文献中引用，规格为200 µg/ml
  • 针对纯化淋巴细胞膜CD44抗原（PGp-1
  • CD44 抗体 (DF1485) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 HCAM
  • 抗CD44抗体（DF1485）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供碱性磷酸酶偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取CD44 (DF1485): sc-7297的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CD44 抗体 (DF1485) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD44 抗体 (DF1485) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

HCAM抗体（DF1485）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和流式细胞术（FCM）检测鼠、大鼠和人源HCAM蛋白。抗HCAM抗体（DF1485）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。HCAM蛋白（也称为CD44）在细胞粘附和迁移中起着关键作用，它充当透明质酸的受体，并与骨桥蛋白等配体相互作用。这种相互作用对于造血、淋巴细胞活化和肿瘤转移等过程至关重要，凸显了HCAM在正常生理功能和病理条件下的重要性。HCAM是一种742个氨基酸的单通道I型膜蛋白，可进行选择性剪接，从而产生多种同种型，包括CD44R、CDw44、CD44S、CD44H和CD44E。CD44H异构体在癌组织中的表达水平较高，表明其与肿瘤进展密切相关，因此HCAM成为治疗干预的潜在靶点。HCAM能够介导细胞与细胞以及细胞与基质的相互作用，这凸显了其在胚胎发育和发育中的重要作用，进一步强调了HCAM (DF1485)抗体在研究和临床应用中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD44 抗体 (DF1485) 参考文献:

  1. 正常唾液腺组织和唾液腺恶性肿瘤中的粘附分子NCAM、HCAM、PECAM-1和ICAM-1。  |  Perschbacher, K., et al. 2004. J Oral Pathol Med. 33: 230-6. PMID: 15061711
  2. CD44 和附件蛋白 A2 介导维生素 D 结合蛋白的 C5a 趋化辅助因子功能。  |  McVoy, LA. and Kew, RR. 2005. J Immunol. 175: 4754-60. PMID: 16177123
  3. CD44 的变异异构体是结肠癌细胞上的 P-和 L-选择素配体。  |  Hanley, WD., et al. 2006. FASEB J. 20: 337-9. PMID: 16352650
  4. 肿瘤细胞通过生成透明质酸片段来增强自身的 CD44 分裂和运动能力。  |  Sugahara, KN., et al. 2006. J Biol Chem. 281: 5861-8. PMID: 16407205
  5. SHAP 可增强 CD44 介导的白细胞对透明质酸基质的粘附。  |  Zhuo, L., et al. 2006. J Biol Chem. 281: 20303-14. PMID: 16702221
  6. 骨桥蛋白和 CD44 作为前列腺癌细胞转移原理的机制。  |  Desai, B., et al. 2007. Mol Cancer. 6: 18. PMID: 17343740
  7. CD44 调节肝细胞生长因子介导的血管完整性。c-Met, Tiam1/Rac1, dynamin 2 和 cortactin 的作用。  |  Singleton, PA., et al. 2007. J Biol Chem. 282: 30643-57. PMID: 17702746
  8. CD44 标准/ezrin 复合物调节 Jurkat 细胞中 Fas 介导的细胞凋亡。  |  Mielgo, A., et al. 2007. Apoptosis. 12: 2051-61. PMID: 17726647
  9. 在具有抗药性的 NB4 细胞系中重新表达 DNA 甲基化沉默的 CD44 基因：cAMP 可挽救 CD44 依赖性细胞死亡。  |  Abecassis, I., et al. 2008. Leukemia. 22: 511-20. PMID: 18094716
  10. 透明质酸合成酶-1的表达通过CD44调控膀胱癌的生长, 侵袭和血管生成。  |  Golshani, R., et al. 2008. Cancer Res. 68: 483-91. PMID: 18199543
  11. 肿瘤性人类组织肥大细胞表达粘附分子CD44/HCAM。  |  Horny, HP., et al. 1996. Virchows Arch. 429: 91-4. PMID: 8917709
  12. 小淋巴细胞和套细胞淋巴瘤中CD44（HCAM）的表达。  |  Aguilera, NS., et al. 1998. Hum Pathol. 29: 1134-9. PMID: 9781654

订购信息

产品名称产品编号规格价格数量收藏夹

CD44 抗体 (DF1485)

sc-7297
200 µg/ml
RMB2377.00

CD44 (DF1485): m-IgG Fc BP-HRP 套装

sc-526494
200 µg Ab; 10 µg BP
RMB2662.00

CD44 (DF1485): m-IgG1 BP-HRP 套装

sc-531867
200 µg Ab; 20 µg BP
RMB2662.00

CD44 抗体 (DF1485) AC

sc-7297 AC
500 µg/ml, 25% agarose
RMB3129.00

CD44 抗体 (DF1485) HRP

sc-7297 HRP
200 µg/ml
RMB2377.00

CD44 抗体 (DF1485) FITC

sc-7297 FITC
200 µg/ml
RMB2482.00

CD44 抗体 (DF1485) PE

sc-7297 PE
200 µg/ml
RMB2580.00

CD44 抗体 (DF1485) Alexa Fluor® 488

sc-7297 AF488
200 µg/ml
RMB2685.00

CD44 抗体 (DF1485) Alexa Fluor® 546

sc-7297 AF546
200 µg/ml
RMB2685.00

CD44 抗体 (DF1485) Alexa Fluor® 594

sc-7297 AF594
200 µg/ml
RMB2685.00

CD44 抗体 (DF1485) Alexa Fluor® 647

sc-7297 AF647
200 µg/ml
RMB2685.00

CD44 抗体 (DF1485) Alexa Fluor® 680

sc-7297 AF680
200 µg/ml
RMB2685.00

CD44 抗体 (DF1485) Alexa Fluor® 790

sc-7297 AF790
200 µg/ml
RMB2685.00

CD44 抗体 (DF1485) APC

sc-7297 APC
100 tests in 2 ml
RMB2640.00

Does Tris-EDTA antigen retrieval buffer work for this antibody?

Asked by: wnwn
Yes,Tris-EDTA is a recommended antigen retrieval method. in section C of our protocol you can see other antigen retrieval methods. https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2025-03-07

I would like more information on the antibody clone: which portions of the protein does it recognize? I am particularly interested in whether the antibody activates the receptor or not.

Asked by: MMunimore
Thank you for your question. Anti-HCAM Antibody (DF1485): sc-7297 was raised against purified CD44 antigen (PGp-1) from lymphocyte membrane. No epitope mapping has been performed as of yet, so I am afraid we do not have any information on the exact epitope it recognises.
Answered by: Tech Support Europe
Date published: 2021-12-07

Is this antibody detecting all the Cd44 variants?

Asked by: TCD2
Thank you for your question. This anitbody was raised against the full length of the protein, we do not characterize the exact binding site of the antibody, and therefore we do not know if the antibody will detect all variants of CD44.
Answered by: Technical Service 4
Date published: 2020-12-18

Does this antibody work under reducing conditions (WB)?

Asked by: hyejinnee
Thank you for your question. Yes, this antibody is fine for reducing denaturing conditions.
Answered by: Tech Service
Date published: 2020-03-16

Hi, can HCAM Antibody (DF1485): sc-7297 be used in flow cytometer for FITC range? Thanks in advance. Regards, Deepak KGK

Asked by: Deepak KGK
Thank you for your question. This antibody is recommended for flow cytometry (FCM), and is available directly conjugated to FITC for this application, sc-7297 FITC.
Answered by: Technical Support
Date published: 2019-07-13

which amino acid region it binds?

Asked by: SChr
Thank you for your question. This mouse monoclonal antibody was raised against purified CD44 antigen (PGp-1) from lymphocyte membrane. The exact epitope (amino acid region) recognized by this antibody has not been determined.
Answered by: Technical Support
Date published: 2021-12-06

Which extraction procedure and inhibitors are recommended? I can't obtain the positive band of 90 kD in hole lysate and membrane fraction of CCRF-CEM cells using HCAM (sc7297) and m-IgGk BP-HRP (sc-516102) secondary AB. 

Asked by: Irina N
Thank you very much for your inquiry. You will find information in our recommended protocols provided online. For additional information, please contact our Technical Service by email, phone or chat live.
Answered by: Technical Support Europe
Date published: 2017-10-26

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service
Date published: 2016-12-23
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_8
  • loc_zh_CN, sid_7297, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 105ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from good antibodyHCAM-DF1485 recognizes a single band in D.rerio lysate at the predicted MW. Nice antibody
Date published: 2021-04-20
Rated 5 out of 5 by from Great IF detection on fixed frozen tissueSample: adult R. macaque liver // Fix: 4% PFA 24hrs // Retrieval: 15mg/mL hyaluronidase for 30mins @37C // Block: 10% serum in Tris-triton (pH 8.0) for 1 hour @RT // Dilution: 1:50 (strong) in 10% serum in Tris-triton // Primary Incubation: overnight @4C // Secondary: Mouse polyclonal, Alexa Fluor 555 (Invitrogen A-31570)
Date published: 2020-02-06
Rated 3 out of 5 by from antibody acceptableuse dilution 1: 50 over night for immunofluorescence
Date published: 2018-04-10
Rated 5 out of 5 by from Works well for WB!Got a nice strong band for human cancer cell line lysate when using 1:200 dilution.
Date published: 2018-03-23
Rated 5 out of 5 by from Good AntibodyUsed a 1:300 dilution for WB with Sum159, Caco-2, and 293T cell lysates. Good signal strength and specific
Date published: 2017-11-09
Rated 1 out of 5 by from IF do not work on embryonic mouse brainIF (1:200) on Cryosection from 4%PFA fixed E13.5 mouse cerebellum, no signal were detected.
Date published: 2017-11-08
Rated 5 out of 5 by from Great for Flow CitometryI received this as a free sample. I tried it with a dilution 1ul of antibody per 200000 cells and I obteined great results!
Date published: 2017-10-12
Rated 5 out of 5 by from Great itemsHCAM Antibody (DF1485) I bought was working very well. I am in my mind that I will order it, when I need it. I am also thinking other antibodies as well. I will order them through SCBT, so sure.
Date published: 2017-10-10
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_19
  • loc_zh_CN, sid_7297, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
CD44 抗体 (DF1485) is rated 4.5 out of 5 by 19.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_19
  • loc_zh_CN, sid_7297, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 102ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。