Date published: 2026-10-24

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HAS2 Antibody (A-7) - Western Blotting - Image 354686
HAS2 抗体 (A-7): sc-514737. HL-60 (A), NIH/3T3 (B), C3H/10T1/2 (C), NRK (D) 和 RPE-J (E) 全细胞裂解液中 HAS2 表达的 Western 印迹分析.
HAS2 抗体 (A-7): sc-514737. HL-60 (A), NIH/3T3 (B), C3H/10T1/2 (C), NRK (D) 和 RPE-J (E) 全细胞裂解液中 HAS2 表达的 Western 印迹分析.

HAS2 抗体 (A-7): sc-514737

5.0(2)
寫評論提問
说明书
  • HAS2 抗体 A-7 是小鼠单克隆 IgG1 κ，HAS2抗体, 在10篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列121-180位于human物种的HAS2的内部区域内
  • HAS2 抗体 (A-7) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 HAS2
  • 抗HAS2抗体（A-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是HAS2 Antibody (A-7) for WB applications. 的首选辅助检测试剂，这些试剂现与HAS2 Antibody (A-7) 打包提供（请参阅下面的订购信息）。
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此外 抗体 包括 和

HAS2抗体（A-7）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的HAS2蛋白。HAS2抗体（A-7）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。透明质酸合成酶家族成员HAS2在透明质酸（HA）的合成中起着至关重要的作用，透明质酸是细胞外基质的重要组成部分，有助于组织的水合作用、弹性和细胞信号传导。透明质酸是一种高分子量线性多糖，由葡萄糖醛酸和N-乙酰葡糖胺残基交替组成，通过β-1,3和β-1,4糖苷键连接。HAS2合成透明质酸在软骨细胞和软骨中尤为重要，透明质酸可支持这些组织的结构完整性和功能。HAS2在生长细胞中的表达显著上调，表明其在发育和组织修复中起着重要作用。人类HAS2基因位于8q24.12染色体上，发育过程中的不同表达模式凸显了其在各种生理过程中的功能意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HAS2 抗体 (A-7) 参考文献:

  1. 透明质酸寡糖处理软骨细胞可刺激 HAS-2 和 MMP-3 的表达，但通过不同的信号途径。  |  Schmitz, I., et al. 2010. Osteoarthritis Cartilage. 18: 447-54. PMID: 19874928
  2. TGFβ诱导的上皮-间质转化能否有效进行取决于透明质酸合成酶 HAS2。  |  Porsch, H., et al. 2013. Oncogene. 32: 4355-65. PMID: 23108409
  3. 抑制 HAS2 的诱导可通过持续的 DNA 损伤增强癌细胞的放射敏感性。  |  Shen, YN., et al. 2014. Biochem Biophys Res Commun. 443: 796-801. PMID: 24333416
  4. 婴儿中伴有PLAG1-HAS2融合的未分化蕈样脂肪母细胞瘤；形态学上模仿婴儿原始蕈样间充质肿瘤（PMMTI）--细胞遗传学和分子检测的诊断重要性及文献综述  |  Warren, M., et al. 2016. Cancer Genet. 209: 21-9. PMID: 26701195
  5. 新型 ZEB1/HAS2 正反馈环促进乳腺癌的 EMT  |  Preca, BT., et al. 2017. Oncotarget. 8: 11530-11543. PMID: 28086235
  6. Has2天然反义RNA和Hmga2在TGFβ诱导的乳腺癌EMT过程中促进Has2的表达。  |  Kolliopoulos, C., et al. 2019. Matrix Biol. 80: 29-45. PMID: 30194979
  7. 长非编码 RNA HAS2-AS1 通过靶向 LSD1/EphB3 通路加速非小细胞肺癌的化疗耐药性  |  Sun, P., et al. 2020. Am J Transl Res. 12: 950-958. PMID: 32269726
  8. 转录因子 USF1 通过激活 lncRNA HAS2-AS1 促进胶质瘤细胞的侵袭和迁移。  |  Wang, J., et al. 2020. Biosci Rep. 40: PMID: 32776110
  9. Hsa_circRNA_102002通过调控miR-488-3p/HAS2轴促进甲状腺乳头状癌的转移。  |  Zhang, W., et al. 2021. Cancer Gene Ther. 28: 279-293. PMID: 32862195
  10. 透明质酸相关基因HAS2、HYAL1-4、PH20和HYALP1与卵巢癌的预后、细胞活力和球形细胞形成能力有关。  |  Riecks, J., et al. 2022. J Cancer Res Clin Oncol. 148: 3399-3419. PMID: 35767191

订购信息

产品名称产品编号规格价格数量收藏夹

HAS2 抗体 (A-7)

sc-514737
200 µg/ml
CNY2422.00

HAS2 (A-7): m-IgG Fc BP-HRP 套装

sc-527935
200 µg Ab; 10 µg BP
CNY2715.00

HAS2 (A-7): m-IgG1 BP-HRP 套装

sc-533308
200 µg Ab; 20 µg BP
CNY2715.00

HAS2 抗体 (A-7) AC

sc-514737 AC
500 µg/ml, 25% agarose
CNY3189.00

HAS2 抗体 (A-7) HRP

sc-514737 HRP
200 µg/ml
CNY2422.00

HAS2 抗体 (A-7) FITC

sc-514737 FITC
200 µg/ml
CNY2527.00

HAS2 抗体 (A-7) PE

sc-514737 PE
200 µg/ml
CNY2625.00

HAS2 抗体 (A-7) Alexa Fluor® 488

sc-514737 AF488
200 µg/ml
CNY2738.00

HAS2 抗体 (A-7) Alexa Fluor® 546

sc-514737 AF546
200 µg/ml
CNY2738.00

HAS2 抗体 (A-7) Alexa Fluor® 594

sc-514737 AF594
200 µg/ml
CNY2738.00

HAS2 抗体 (A-7) Alexa Fluor® 647

sc-514737 AF647
200 µg/ml
CNY2738.00

HAS2 抗体 (A-7) Alexa Fluor® 680

sc-514737 AF680
200 µg/ml
CNY2738.00

HAS2 抗体 (A-7) Alexa Fluor® 790

sc-514737 AF790
200 µg/ml
CNY2738.00

What is the expected kDA band size for this HAS2 antibody?

Asked by: Kristen N
Thank you for your question. The molecular weight is 63KDa.
Answered by: BlakeJ
Date published: 2026-01-09

Can HAS2 (A-7): sc-514737 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, HAS2 (A-7): sc-514737 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

Hallo, wie wurden die Zellen für den WB lysiert (welcher Lysepuffer, Welche Aufbereitung)? Wurden die Proben vor der Elektrophorese reduziert? Wieviel Protein wurde je Spur aufgetragen?

Asked by: UlfA
Thank you very much for your inquiry. We use RIPA buffer for sample preparation and the Western Blot was performed under reducing conditions. We recommend to load 40–60 µg whole cell lysate per lane according to our protocol for Western Blotting. Please find further information in our protocol under the following link: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting If you should have any additional questions please contact our European Technical Service team. You can reach us by phone at: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com We are happy to assist you.
Answered by: Technical Support Europe
Date published: 2017-02-01
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Rated 5 out of 5 by AS2017 from clean antibody for WBThis antibody worked really well for my WB experiment. I used it for MDA-MB231 cell lysates, it just picked only one crisp band exactly at 63kDa, as suggested by data sheet. I used 1:800 dilution. Over all the blot was clean and there was no non-specific bands either.
Date published: 2017-10-16
Rated 5 out of 5 by DefinitelyNotMatt from Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-08
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HAS2 抗体 (A-7) is rated 5.0 out of 5 by 2.
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