Date published: 2025-9-7
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GTBP Antibody (E-8) - Western Blotting - Image 164447
GTBP 抗体 (E-8): sc-137015. HeLa (A), A-431 (B), SW480 (C), K-562 (D), DU 145 (E) 和 Jurkat (F) 核提取物中 GTBP 表达的 Western 印迹分析.
GTBP 抗体 (E-8): sc-137015. HeLa (A), A-431 (B), SW480 (C), K-562 (D), DU 145 (E) 和 Jurkat (F) 核提取物中 GTBP 表达的 Western 印迹分析.

GTBP 抗体 (E-8): sc-137015

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说明书
  • GTBP 抗体 E-8 是小鼠单克隆 IgG2a κ，GTBP抗体, 在3篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1220-1360，在GTBP (G/T binding protein)的human起源的C-terminus处进行映射
  • GTBP 抗体 (E-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GTBP
  • 抗GTBP抗体（E-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GTBP (E-8): sc-137015的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 GTBP 抗体 (E-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GTBP 抗体 (E-8) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

GTBP抗体（E-8）是一种IgG2a κ小鼠单克隆GTBP抗体，可通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人源的GTBP蛋白。GTBP抗体（E-8）既可以是非偶联的抗GTBP抗体形式，也可以是多种偶联形式的抗GTBP抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。发现DNA错配修复基因的突变与遗传性非息肉病性结直肠癌（HNPCC）有关，引起了人们对DNA错配修复机制理解的极大兴趣。最初，在HNPCC中高频率地证明了细菌DNA错配修复基因mutS和mutL的MSH2和MLH1同源物中的遗传性突变，并显示其与微卫星不稳定性有关。错配修复家族的一个成员GTBP（也称为MSH6）是一种与MSH2相关的蛋白质，可与含有G/T错配的DNA结合。研究结果表明，人细胞中的错配结合因子由GTBP和MSH2的异二聚体组成。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GTBP 抗体 (E-8) 参考文献:

  1. 晚期上皮性卵巢癌中 hMSH2 和 GTBP 的表达。  |  Ercoli, A., et al. 1999. Br J Cancer. 80: 1665-71. PMID: 10408416
  2. 错配修复基因（hMLH1、hPMS1、hPMS2、GTBP/hMSH6、hMSH2）在肝细胞癌发病机制中的作用。  |  Zekri, AR., et al. 2005. World J Gastroenterol. 11: 3020-6. PMID: 15918183
  3. GTBP，一种对人类细胞错配结合活性至关重要的 160 千道尔顿蛋白质。  |  Palombo, F., et al. 1995. Science. 268: 1912-4. PMID: 7604265
  4. 基因不稳定细胞中的 GTBP 突变。  |  Papadopoulos, N., et al. 1995. Science. 268: 1915-7. PMID: 7604266
  5. 酵母在启动 DNA 错配修复过程中与 MLH1, PMS1 和 MSH2 的相互作用  |  Prolla, TA., et al. 1994. Science. 265: 1091-3. PMID: 8066446
  6. 遗传性非息肉病结肠癌中两种 PMS 同源物的突变。  |  Nicolaides, NC., et al. 1994. Nature. 371: 75-80. PMID: 8072530
  7. 错配修复与癌症  |  Palombo, F., et al. 1994. Nature. 367: 417. PMID: 8107798
  8. 遗传性结肠癌中的 mutL 同源物突变。  |  Papadopoulos, N., et al. 1994. Science. 263: 1625-9. PMID: 8128251
  9. DNA 错配修复基因同源物 hMLH1 的突变与遗传性非息肉病结肠癌缔合作用。  |  Bronner, CE., et al. 1994. Nature. 368: 258-61. PMID: 8145827
  10. 人类大肠癌易感基因座的基因图谱。  |  Peltomäki, P., et al. 1993. Science. 260: 810-2. PMID: 8484120
  11. 非典型遗传性非息肉病结直肠癌种系中 hMSH6/GTBP 基因的种系突变。  |  Akiyama, Y., et al. 1997. Cancer Res. 57: 3920-3. PMID: 9307272
  12. 正常人体组织中 GTBP 的交替剪接。  |  Shiwaku, HO., et al. 1997. DNA Res. 4: 359-62. PMID: 9455487

订购信息

产品名称产品编号规格价格数量收藏夹

GTBP 抗体 (E-8)

sc-137015
200 µg/ml
RMB2377.00

GTBP (E-8): m-IgG Fc BP-HRP 套装

sc-528837
200 µg Ab; 10 µg BP
RMB2662.00

GTBP (E-8): m-IgGκ BP-HRP 套装

sc-521395
200 µg Ab, 40 µg BP
RMB2662.00

GTBP (E-8): m-IgG2a BP-HRP 套装

sc-547181
200 µg Ab; 10 µg BP
RMB2662.00

GTBP 抗体 (E-8) AC

sc-137015 AC
500 µg/ml, 25% agarose
RMB3129.00

GTBP 抗体 (E-8) HRP

sc-137015 HRP
200 µg/ml
RMB2377.00

GTBP 抗体 (E-8) FITC

sc-137015 FITC
200 µg/ml
RMB2482.00

GTBP 抗体 (E-8) PE

sc-137015 PE
200 µg/ml
RMB2580.00

GTBP 抗体 (E-8) Alexa Fluor® 488

sc-137015 AF488
200 µg/ml
RMB2685.00

GTBP 抗体 (E-8) Alexa Fluor® 546

sc-137015 AF546
200 µg/ml
RMB2685.00

GTBP 抗体 (E-8) Alexa Fluor® 594

sc-137015 AF594
200 µg/ml
RMB2685.00

GTBP 抗体 (E-8) Alexa Fluor® 647

sc-137015 AF647
200 µg/ml
RMB2685.00

GTBP 抗体 (E-8) Alexa Fluor® 680

sc-137015 AF680
200 µg/ml
RMB2685.00

GTBP 抗体 (E-8) Alexa Fluor® 790

sc-137015 AF790
200 µg/ml
RMB2685.00

Can GTBP (E-8): sc-137015 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, GTBP (E-8): sc-137015 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Great featureI requested this antibody for free a couple weeks ago, the first time I run WB as 1:100, it works well, but has some non-specific bands. After that, the antibody works fine, one specific band appears around 180kD in my Hela cell samples in shorter exposure time.
Date published: 2017-10-03
Rated 3 out of 5 by from GTBP/Msh6 antibody (E-8) in Western blottingI received a sample antibody and tried it at a 1:100 dilution to probe whole cell extracts from various cell lines as labelled in my picture (note that GTBP runs at ~160 kDa). While it did not work in Mouse Embryonic Fibroblast (MEF) cells, it did work in human cell lines like HeLa, HEK293, and HEK293T (also known as Phoenix cells). There were some non-specific bands from this antibody, as seen in my western blot. If I were to buy this antibody or receive another sample, I might try a lower concentration next time. Overall, I'd say it's not bad.
Date published: 2017-09-05
Rated 5 out of 5 by from GreatThe antibody is pretty good for IF staining. The background is very low
Date published: 2017-08-02
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclearProduced excellent immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-02-02
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GTBP抗体 (E-8) is rated 4.6 out of 5 by 5.
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