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GST Antibody (3D4) - Western Blotting - Image 15622
GST 抗体 (3D4): sc-57753. 重组 GST 蛋白的 Western 印迹分析.
GST Antibody (3D4) - Western Blotting - Image 15622
GST 抗体 (3D4): sc-57753. 重组 GST 蛋白的 Western 印迹分析.
GST 抗体 (3D4): sc-57753. 重组 GST 蛋白的 Western 印迹分析.

GST 抗体 (3D4): sc-57753

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说明书
  • GST 抗体 3D4 是小鼠单克隆 IgG1, 在15篇文献中引用，提供 100 µg/ml
  • 免疫S. japonicum物种的GST
  • 推荐用于 Schistosoma japonicum 来源的GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin WB, IP 和 IF检测
  • 看 GST (B-14): sc-138，可查看有标记的 GST 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成GST 抗体 (3D4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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在大肠杆菌中表达真核基因编码区的质粒载体是常用的；这种表达载体通常编码含有部分原核和部分真核特异蛋白的杂合融合蛋白。例如，Smith和Johnson开发的pGEX.3X表达载体允许合成谷胱甘肽-S-转移酶（GST）和插入的cDNA序列编码的蛋白质之间的融合蛋白。源自这些GST融合蛋白的抗体可用于检查斑块和蛋白质印迹中的蛋白质表达，以及用于大肠杆菌中表达的蛋白质的免疫亲和纯化。

仅限研究使用。不适用于诊断和治疗用途。

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产品名称产品编号规格价格数量收藏夹

GST 抗体 (3D4)

sc-57753
100 µg/ml
RMB2377.00

what is recommended amount for IP?

Asked by: mac1982
Thank you for your question. To 1 ml of the above cell lysate, or approximately 100–1000 µg of total cellular protein, add 10 µg of primary antibody agarose conjugate (i.e., 5 µl volume of packed beads). You can find our recommended protocol for immunoprecipitation by following this link: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What is the total volume provided of GST (3D4): sc-57753 monoclonal antibody?

Asked by: Trav11
Thank you for your question. This antibody is provided as 100 µg in 1 ml total volume.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Published WB data using the following: 293T cellsPublished WB data using the following: 293T cells, HeLa cells, A431 cells, SW480 cells, RKO cells, SaOs cells, and MG63 cells. -SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by from NiceNice, publishable WB data with 3D4. -SCBT Publication Review
Date published: 2015-03-31
Rated 5 out of 5 by from Strong band observed in Western blot analysisStrong band observed in Western blot analysis of recombinant GST protein. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Published data using 293T cells for Western blotPublished data using 293T cells for Western blot. -SCBT Publication Review
Date published: 2015-02-24
Rated 5 out of 5 by from Great western blot results when with HumanGreat western blot results when with Human embryonic fibroblast (HE) cells. -SCBT Publication Review
Date published: 2013-03-20
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GST 抗体 (3D4) is rated 5.0 out of 5 by 5.
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