Date published: 2025-9-6

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GRB2 抗体 (C-7): sc-8034

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说明书
  • GRB2 抗体 C-7 是小鼠单克隆 IgG1 κ,GRB2抗体, 在48篇文献中引用,规格为200 µg/ml
  • 与氨基酸54-164结合,映射到mouse起源的GRB2的中心域
  • GRB2 抗体 (C-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 GRB2 p25
  • 抗GRB2抗体(C-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GRB2 (C-7): sc-8034免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 GRB2 抗体 (C-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GRB2 抗体 (C-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

GRB2抗体(C-7)是一种鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人源的GRB2蛋白。GRB2单克隆抗体(C-7)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。GRB2蛋白在细胞信号传导通路中发挥着关键作用,特别是在Ras信号级联的调节中,而Ras信号级联对于细胞生长、分化及存活至关重要。作为连接蛋白,GRB2促进生长因子受体与SOS1等鸟嘌呤核苷酸交换因子之间的相互作用,从而促进Ras响应细胞外信号而激活。这种相互作用对于信号从细胞表面正确传递到细胞核至关重要,影响各种细胞过程。此外,GRB2在胚胎发育过程中对于内胚层细胞分化以及上皮层形成必不可少,凸显其在发育生物学中的重要性。GRB2单克隆抗体(C-7)能够有效检测和鉴定GRB2蛋白,因此GRB2抗体(C-7)对于研究信号通路及其对健康和疾病的影响的研究人员来说是一种宝贵的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GRB2 抗体 (C-7) 参考文献:

  1. Grb2 SH2 域结合肽类似物作为潜在的抗癌剂。  |  Lung, FD. and Tsai, JY. 2003. Biopolymers. 71: 132-40. PMID: 12767115
  2. 含SH2和SH3结构域的蛋白质GRB2将受体酪氨酸激酶与ras信号传导联系起来。  |  Lowenstein, EJ., et al. 1992. Cell. 70: 431-42. PMID: 1322798
  3. GRB2是一种与BECN1相互作用的蛋白质,可调节自噬。  |  Montero-Vergara, J., et al. 2024. Cell Death Dis. 15: 14. PMID: 38182563
  4. GRB2在逆转复制叉中稳定RAD51,抑制基因组不稳定性,增强癌症的先天免疫。  |  Ye, Z., et al. 2024. Nat Commun. 15: 2132. PMID: 38459011
  5. GRB2在蛋白质相互作用和信号转导中的配置。  |  Wang, D., et al. 2024. Biomolecules. 14: PMID: 38540680
  6. GRB2在糖尿病、糖尿病并发症及相关疾病中的作用。  |  Ma, J., et al. 2025. Diabetes Obes Metab. 27: 23-34. PMID: 39478285
  7. GRB2 在连接胰岛素受体和 Ras 信号通路方面的功能。  |  Skolnik, EY., et al. 1993. Science. 260: 1953-5. PMID: 8316835
  8. 正常和致癌的 p21ras 蛋白与 c-Raf-1 的氨基末端,N 端调节结构域结合。  |  Zhang, XF., et al. 1993. Nature. 364: 308-13. PMID: 8332187
  9. 一种 SH3-SH2-SH3 蛋白是 p21Ras1 激活所必需的,并能在体外与 sevenless 和 Sos 蛋白结合。  |  Simon, MA., et al. 1993. Cell. 73: 169-77. PMID: 8462097
  10. Sos Ras 交换蛋白与 Grb2 的缔合作用与酪氨酸激酶信号转导和转化有关。  |  Egan, SE., et al. 1993. Nature. 363: 45-51. PMID: 8479536
  11. 表皮生长因子通过形成由受体, Grb2 适配蛋白和 Sos 核苷酸交换因子组成的复合物来调节 p21ras。  |  Buday, L. and Downward, J. 1993. Cell. 73: 611-20. PMID: 8490966
  12. 人类 Sos1:与 GRB2 结合的 Ras 的鸟嘌呤核苷酸交换因子。  |  Chardin, P., et al. 1993. Science. 260: 1338-43. PMID: 8493579

订购信息

产品名称产品编号规格价格数量收藏夹

GRB2 抗体 (C-7)

sc-8034
200 µg/ml
RMB2377.00

GRB2 (C-7): m-IgG Fc BP-HRP 套装

sc-528209
200 µg Ab; 10 µg BP
RMB2662.00

GRB2 (C-7): m-IgGκ BP-HRP 套装

sc-520532
200 µg Ab, 40 µg BP
RMB2662.00

GRB2 (C-7): m-IgG1 BP-HRP 套装

sc-542818
200 µg Ab; 20 µg BP
RMB2662.00

GRB2 抗体 (C-7) AC

sc-8034 AC
500 µg/ml, 25% agarose
RMB3129.00

GRB2 抗体 (C-7) HRP

sc-8034 HRP
200 µg/ml
RMB2377.00

GRB2 抗体 (C-7) FITC

sc-8034 FITC
200 µg/ml
RMB2482.00

GRB2 抗体 (C-7) PE

sc-8034 PE
200 µg/ml
RMB2580.00

GRB2 抗体 (C-7) Alexa Fluor® 488

sc-8034 AF488
200 µg/ml
RMB2685.00

GRB2 抗体 (C-7) Alexa Fluor® 546

sc-8034 AF546
200 µg/ml
RMB2685.00

GRB2 抗体 (C-7) Alexa Fluor® 594

sc-8034 AF594
200 µg/ml
RMB2685.00

GRB2 抗体 (C-7) Alexa Fluor® 647

sc-8034 AF647
200 µg/ml
RMB2685.00

GRB2 抗体 (C-7) Alexa Fluor® 680

sc-8034 AF680
200 µg/ml
RMB2685.00

GRB2 抗体 (C-7) Alexa Fluor® 790

sc-8034 AF790
200 µg/ml
RMB2685.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 4 out of 5 by from work well for WBI used this antibody for WB with recommended dilution ratio. And it worked well for all the samples with very clear and specific bands. My samples included both cell lines (MEF and SY5Y) and mice tissues (liver, lung and kidney).
Date published: 2018-03-15
Rated 4 out of 5 by from Good for WBI used it in mouse lymphocytes and worked well for WB.
Date published: 2018-02-14
Rated 5 out of 5 by from SalThe antibody work fine I hav tried with whole cell lysate from Jurkat T cells
Date published: 2017-10-17
Rated 4 out of 5 by from Great antibody in specificityIt works well in WB at 1:400, C2C12 cells and has a good specificity.
Date published: 2017-08-17
Rated 5 out of 5 by from Excellent antibodyWe get pretty results in WB, with a strong, clean 25 kDa band and one weak additional band below.The background is clean. The working dilution is 1: 500.
Date published: 2017-06-03
Rated 5 out of 5 by from Worked well in WBIt shows a great sensitivity and specificity in WB, I recommend this product.
Date published: 2017-05-13
Rated 5 out of 5 by from Good WBUsed 1:1000 for Western blot in human tonsil tissue, got expected bands
Date published: 2017-04-22
Rated 5 out of 5 by from Outstanding WB and IFGreat western data; strong, clear signal; also nice IF staining
Date published: 2017-01-27
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GRB2 抗体 (C-7) is rated 4.7 out of 5 by 12.
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