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CD130/gp130 Antibody (AN-H2) - Immunohistochemistry - Image 156594
gp130 抗体 (AN-H2): sc-9994. 对福尔马林固定, 石蜡包埋的人心肌组织进行免疫过氧化物酶染色, 显示心肌细胞的胞浆染色.
gp130 抗体 (AN-H2): sc-9994. 对福尔马林固定, 石蜡包埋的人心肌组织进行免疫过氧化物酶染色, 显示心肌细胞的胞浆染色.

CD130/gp130 抗体 (AN-H2): sc-9994

4.8(5)
寫評論提問

说明书
  • CD130/gp130抗体(AN-H2)是小鼠单克隆IgG1 κ, 在10篇文献中引用，规格为200 µg/ml
  • 针对gp130 human来源的Sf21细胞
  • CD130/gp130 抗体 (AN-H2) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA，检测human 来源的 gp130
  • 抗CD130/gp130抗体（AN-H2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD130/gp130 (AN-H2): sc-9994的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 CD130/gp130 抗体 (AN-H2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD130/gp130 抗体 (AN-H2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

gp130 (AN-H2) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ 酶联免疫吸附试验（ELISA）。gp130单克隆抗体（AN-H2）有非结合型以及多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。IL-6通过与由配体结合蛋白（IL-6R）和另一种蛋白组成的受体结合来激活细胞内信号传导。IL-6首先与IL-6R结合，随后与gp130二聚体结合。活性信号传导复合物至少由IL-6、IL-6R和由二硫键连接的两个gp130蛋白二聚体组成。膜结合前体通过蛋白水解作用产生可溶性IL-6R，可作为激动剂分子积极参与细胞间信号传导。IL-6复合物的第二个亚基gp130也是多种其他受体复合物的组成部分，包括白血病抑制因子（LIF）、肿瘤抑制素M（OSM）、纤毛神经营养因子（CNTF）和IL-11。LIF与LIF受体的结合力较弱，与LIF受体和gp130的复合物的结合力较强，而OSM与gp130的结合力较弱，与gp130和LIF受体的复合物的结合力较强。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD130/gp130 抗体 (AN-H2)

sc-9994
200 µg/ml
RMB2377.00

CD130/gp130 (AN-H2): m-IgG Fc BP-HRP 套装

sc-528249
200 µg Ab; 10 µg BP
RMB2662.00

CD130/gp130 (AN-H2): m-IgGκ BP-HRP 套装

sc-520578
200 µg Ab, 40 µg BP
RMB2662.00

CD130/gp130 (AN-H2): m-IgG1 BP-HRP 套装

sc-542839
200 µg Ab; 20 µg BP
RMB2662.00

CD130/gp130 抗体 (AN-H2) AC

sc-9994 AC
500 µg/ml, 25% agarose
RMB3129.00

CD130/gp130 抗体 (AN-H2) HRP

sc-9994 HRP
200 µg/ml
RMB2377.00

CD130/gp130 抗体 (AN-H2) FITC

sc-9994 FITC
200 µg/ml
RMB2482.00

CD130/gp130 抗体 (AN-H2) PE

sc-9994 PE
200 µg/ml
RMB2580.00

CD130/gp130 抗体 (AN-H2) Alexa Fluor® 488

sc-9994 AF488
200 µg/ml
RMB2685.00

CD130/gp130 抗体 (AN-H2) Alexa Fluor® 546

sc-9994 AF546
200 µg/ml
RMB2685.00

CD130/gp130 抗体 (AN-H2) Alexa Fluor® 594

sc-9994 AF594
200 µg/ml
RMB2685.00

CD130/gp130 抗体 (AN-H2) Alexa Fluor® 647

sc-9994 AF647
200 µg/ml
RMB2685.00

CD130/gp130 抗体 (AN-H2) Alexa Fluor® 680

sc-9994 AF680
200 µg/ml
RMB2685.00

CD130/gp130 抗体 (AN-H2) Alexa Fluor® 790

sc-9994 AF790
200 µg/ml
RMB2685.00

Can gp130 (AN-H2): sc-9994 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, gp130 (AN-H2): sc-9994 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent Antibody!The gp130 Antibody (AN-H2): sc-9994 works very well for IHC when working with formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited multiple times for further verification.
Date published: 2017-01-25
Rated 5 out of 5 by from Publishable IHC data in various human retinalPublishable IHC data in various human retinal pigment epithelium. -SCBT Publication Review
Date published: 2015-06-12
Rated 4 out of 5 by from Great IHC data published using NSCLCGreat IHC data published using NSCLC. -SCBT Publication Review
Date published: 2015-04-06
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human heart muscle tissue. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from Produced great FCM data for human peripheralProduced great FCM data for human peripheral blood leukocytes. -SCBT QC
Date published: 2013-11-05
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CD130/gp130 抗体 (AN-H2) is rated 4.8 out of 5 by 5.
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