Date published: 2025-9-6
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CD130/gp130 Antibody (E-8) - Western Blotting - Image 374869
gp130 抗体 (E-8): sc-376280. NIH/3T3 (A), Caco-2 (B) 和 A-10 (C) 全细胞裂解液中 gp130 表达的 Western 印迹分析.
CD130/gp130 Antibody (E-8) - Western Blotting - Image 374869
gp130 抗体 (E-8): sc-376280. NIH/3T3 (A), Caco-2 (B) 和 A-10 (C) 全细胞裂解液中 gp130 表达的 Western 印迹分析.
CD130/gp130 Antibody (E-8) - Immunohistochemistry - Image 157958
gp130 抗体 (E-8): sc-376280. 福尔马林固定, 石蜡包埋的人肾上腺组织的免疫过氧化物酶染色显示腺细胞的胞浆染色.
CD130/gp130 Antibody (E-8) - Western Blotting - Image 155016
gp130 抗体 (E-8): sc-376280. 人胎盘组织提取物 (A) 和 HeLa (B), WEHI-231 (C) 和 PC-12 (D) 全细胞裂解液中 gp130 表达的 Western 印迹分析.
gp130 抗体 (E-8): sc-376280. NIH/3T3 (A), Caco-2 (B) 和 A-10 (C) 全细胞裂解液中 gp130 表达的 Western 印迹分析.

CD130/gp130 抗体 (E-8): sc-376280

4.1(12)
寫評論提問

说明书
  • CD130/gp130 抗体 E-8 是小鼠单克隆 IgG2a κ，CD130/gp130抗体, 在31篇文献中引用，规格为200 µg/ml
  • 免疫human物种的gp130的氨基酸365-619
  • CD130/gp130 抗体 (E-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 gp130
  • 抗CD130/gp130抗体（E-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD130/gp130 (E-8): sc-376280的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 CD130/gp130 抗体 (E-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD130/gp130 抗体 (E-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

gp130抗体（E-8）是一种IgG2a κ小鼠单克隆gp130抗体（也被指定为IL6ST抗体、白细胞介素6信号转导抗体或CD130抗体），它可以通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人类来源的gp130蛋白。gp130抗体（E-8）既可以以非偶联的抗gp130抗体形式提供，也可以以多种偶联形式的抗gp130抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。白细胞介素-6（IL-6）通过结合由配体结合蛋白（IL-6R）和第二种蛋白质组成的受体来激活细胞内信号传导。IL-6首先与IL-6R结合，随后与gp130二聚体结合。活性信号复合物至少由IL-6、IL-6R和通过二硫键连接的两个gp130蛋白质的二聚体组成。可溶性IL-6R是通过膜结合前体的蛋白水解裂解产生的，并可以作为激动剂分子，积极参与细胞间信号传导。IL-6复合物的第二个亚基gp130，也作为几种其他受体复合物的组成部分，包括白血病抑制因子（LIF）、抑瘤素M（OSM）、睫状神经营养因子（CNTF）和IL-11。LIF以低亲和力与LIF受体结合，以高亲和力与LIF受体和gp130的复合物结合，而OSM似乎以低亲和力与gp130结合，以高亲和力与gp130和LIF受体的复合物结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD130/gp130 抗体 (E-8) 参考文献:

  1. 条件性gp130缺陷小鼠突变体。  |  Fasnacht, N. and Müller, W. 2008. Semin Cell Dev Biol. 19: 379-84. PMID: 18687405
  2. 巨噬细胞/粒细胞上的gp130在实验性结核病期间调节炎症。  |  Sodenkamp, J., et al. 2011. Eur J Cell Biol. 90: 505-14. PMID: 21144615
  3. IL-6 信号转导子 gp130 的分子克隆和表达。  |  Hibi, M., et al. 1990. Cell. 63: 1149-57. PMID: 2261637
  4. 白细胞介素-6 会触发其受体与可能的信号转导物 gp130 的结合。  |  Taga, T., et al. 1989. Cell. 58: 573-81. PMID: 2788034
  5. 人白细胞介素-6（BSF-2/IFN beta 2）受体的克隆与表达。  |  Yamasaki, K., et al. 1988. Science. 241: 825-8. PMID: 3136546
  6. 乳腺癌和骨骼中的GP130细胞因子。  |  Omokehinde, T. and Johnson, RW. 2020. Cancers (Basel). 12: PMID: 32023849
  7. 子宫上皮 Gp130 协调激素反应和上皮重塑，使小鼠胚胎成功附着。  |  Namiki, T., et al. 2023. Sci Rep. 13: 854. PMID: 36646738
  8. 小鼠 IL-11 受体α-链的克隆；高亲和力结合和信号转导需要 gp130。  |  Hilton, DJ., et al. 1994. EMBO J. 13: 4765-75. PMID: 7957045
  9. 可溶性人类 IL-6 受体。蛋白水解裂解位点的突变特征。  |  Müllberg, J., et al. 1994. J Immunol. 152: 4958-68. PMID: 8176214
  10. 细胞因子信号转导  |  Kishimoto, T., et al. 1994. Cell. 76: 253-62. PMID: 8293462
  11. LIFR beta 和 gp130 作为三方 CNTF 受体的异源二聚体信号转导子。  |  Davis, S., et al. 1993. Science. 260: 1805-8. PMID: 8390097
  12. 白细胞介素 6 信号转导子 gp130 的关键细胞质区域在细胞因子受体家族中是保守的。  |  Murakami, M., et al. 1991. Proc Natl Acad Sci U S A. 88: 11349-53. PMID: 1662392

订购信息

产品名称产品编号规格价格数量收藏夹

CD130/gp130 抗体 (E-8)

sc-376280
200 µg/ml
RMB2377.00

CD130/gp130 (E-8): m-IgG Fc BP-HRP 套装

sc-529848
200 µg Ab; 10 µg BP
RMB2662.00

CD130/gp130 (E-8): m-IgGκ BP-HRP 套装

sc-522907
200 µg Ab, 40 µg BP
RMB2662.00

CD130/gp130 抗体 (E-8) AC

sc-376280 AC
500 µg/ml, 25% agarose
RMB3129.00

CD130/gp130 抗体 (E-8) HRP

sc-376280 HRP
200 µg/ml
RMB2377.00

CD130/gp130 抗体 (E-8) FITC

sc-376280 FITC
200 µg/ml
RMB2482.00

CD130/gp130 抗体 (E-8) PE

sc-376280 PE
200 µg/ml
RMB2580.00

CD130/gp130 抗体 (E-8) Alexa Fluor® 488

sc-376280 AF488
200 µg/ml
RMB2685.00

CD130/gp130 抗体 (E-8) Alexa Fluor® 546

sc-376280 AF546
200 µg/ml
RMB2685.00

CD130/gp130 抗体 (E-8) Alexa Fluor® 594

sc-376280 AF594
200 µg/ml
RMB2685.00

CD130/gp130 抗体 (E-8) Alexa Fluor® 647

sc-376280 AF647
200 µg/ml
RMB2685.00

CD130/gp130 抗体 (E-8) Alexa Fluor® 680

sc-376280 AF680
200 µg/ml
RMB2685.00

CD130/gp130 抗体 (E-8) Alexa Fluor® 790

sc-376280 AF790
200 µg/ml
RMB2685.00

gp130做石蜡切片免疫组化的抗原修复用碱修复还是酸修复？

Asked by: ANNA
感谢您的提问。酸修复液和碱修复液都可以用于抗原修复。您可以查看我们IHC实验流程进一步了解。实验流程链接如下：https://www.scbt.com/zh/resources/protocols/immunoperoxidase-staining Thank you for your question. Both acid and alkali repair solutions can be used for antigen repair. You can check out our IHC experimental procedures for further information. The experimental process link as follows: https://www.scbt.com/zh/resources/protocols/immunoperoxidase-staining
Answered by: Zhanglong
Date published: 2024-07-22

Does gp130 Antibody (E-8): sc-376280 react with canine samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 5
Date published: 2017-01-16

I would like to use gp130 Antibody (E-8): sc-376280 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-376280 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Service 11
Date published: 2017-01-16
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Rated 1 out of 5 by from gp130(E-8):sc-376280について食道癌細胞をIL-6で刺激してgp130のリン酸化を、p-gp130(A-12):sc-377572とgp130(E-8)の10ug無料サンプル抗体を用いて行いました。p-gp130(A-12)の方は、130kDa付近に綺麗なバンドが検出できました。しかし、gp130(E-8)の抗体では、ノーバンドでした。そこで、gp130の他の抗体、C-20, H-255,　M-20のいずれかの10ug無料サンプルを頂くことは可能でしょうか？
Date published: 2024-04-16
Rated 5 out of 5 by from Very nice resultThe expression of gp130 protein in rat serum was detected by this antibody use ELISA.The antibody was diluted at 1;500. We found the gp130 level is lower in the treated group.
Date published: 2017-09-16
Rated 5 out of 5 by from GOODI have applied for a smaple from santa cruz. I use it in Immunohistochemistry and the result was good。
Date published: 2017-09-05
Rated 1 out of 5 by from GP130I used this antibody in WB at 1:100. Many bands appeared. I don't know which band is gp130.
Date published: 2017-09-04
Rated 3 out of 5 by from OK antibodyGives ok signaling, not good for reusing though. I would suggest use at 1:100
Date published: 2017-08-24
Rated 5 out of 5 by from GOOD !WE USED IN THE WESTERN BLOT,THE RESULT IS GOOD! ANTIBODY IS VERY GOOD!
Date published: 2017-07-05
Rated 4 out of 5 by from gp130I have applied for a smaple from santa cruz. I use it in westernblot and the line in the WB was not very clear.
Date published: 2017-02-20
Rated 5 out of 5 by from the result is satisfactoryThe antibodies in a good western blot gp130 expression in human placenta tissue data extraction and hela, satisfied results
Date published: 2017-02-03
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CD130/gp130 抗体 (E-8) is rated 4.1 out of 5 by 12.
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