Date published: 2025-9-7
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glypican-3 Antibody (1G12) - Western Blotting - Image 54845
glypican-3 抗体 (1G12): sc-65443. 293T 全细胞裂解液中 glypican-3 表达的 Western 印迹分析.
glypican-3 Antibody (1G12) - Western Blotting - Image 54845
glypican-3 抗体 (1G12): sc-65443. 293T 全细胞裂解液中 glypican-3 表达的 Western 印迹分析.
glypican-3 Antibody (1G12) - Western Blotting - Image 109737
glypican-3 抗体 (1G12): sc-65443. 非转染: sc-117752 (A) 和转染: sc-176285 (B) 293T 全细胞裂解液中 glypican-3 表达的 Western 印迹分析.
glypican-3 抗体 (1G12): sc-65443. 293T 全细胞裂解液中 glypican-3 表达的 Western 印迹分析.

glypican-3 抗体 (1G12): sc-65443

5.0(5)
寫評論提問

说明书
  • glypican-3 抗体 1G12 是小鼠单克隆 IgG1, 在30篇文献中引用，提供 15 µg/100 µl
  • 与含有human来源的glypican-3的氨基酸510-580的片段杂交
  • 推荐用于 human 来源的glypican-3 WB, IP, IF 和 IHC(P)检测
  • 看 glypican-3 (F-3): sc-390587，可查看有标记的 glypican-3 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成glypican-3 抗体 (1G12)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 glypican 抗体包括 glypican-1, glypican-2, glypican-3, glypican-5 和 glypican-4

Glypican-3（GPC3）是一种整体膜蛋白，在Simpson-Golabi-Behmel综合征（SGBS）中发生突变。SGBS的特征是出生前和出生后的过度生长，是一种隐性X连锁疾病。glypican-3在大鼠中也称为OCI-5，是硫酸乙酰肝素蛋白多糖的glypican家族成员，通过糖基磷脂酰肌醇（GPI）锚定连接到细胞膜上。在胚胎中胚层肺、肝和肾组织中检测到glypican-3的表达。glypican-3被认为通过与胰岛素样生长因子II（IGF-II）或成纤维细胞生长因子2（FGF-2）等生长因子的相互作用来调节组织和器官的生长。glypican-3可以通过多种方式下调，包括启动子超甲基化或特定转录因子的抑制。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

glypican-3 抗体 (1G12)

sc-65443
15 µg/100 µl
RMB2377.00

Não estou conseguindo detecção para WB, tem alguma recomendação de diluição mais especifica, de bloqueio e diluente para o anticorpo

Asked by: FFMF
Thank you for your question. Please contact our technical service team for troubleshooting advice on your assay.
Answered by: Tech Support Europe
Date published: 2022-09-05

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Sweetums
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 4
Date published: 2016-12-16

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Asked by: Sweetums
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 4
Date published: 2016-12-16
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Rated 5 out of 5 by from very strong signalNice western blot data, clear background effect is very good
Date published: 2017-05-23
Rated 5 out of 5 by from Works great in Western Blot!This antibody works great in western blot analysis of glypican-3 expression in 293T whole cell lysate.
Date published: 2016-12-16
Rated 5 out of 5 by from Works very well by IHC(P)Works very well by IHC(P) - Published data
Date published: 2015-08-04
Rated 5 out of 5 by from Worked well in WB in human glypicanWorked well in WB in human glypican-3 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-01-07
Rated 5 out of 5 by from ClearClear, strong band seen in WB in 293T whole cell lysate. -SCBT QC
Date published: 2013-06-16
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glypican-3 抗体 (1G12) is rated 5.0 out of 5 by 5.
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