Date published: 2025-9-7
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glypican-3 Antibody (F-3) - Western Blotting - Image 289590
glypican-3 抗体 (F-3): sc-390587. COLO 320DM (A), SW480 (B) 和 HTC-116 (C) 全细胞裂解液中 glypican-3 表达的 Western 印迹分析.
glypican-3 抗体 (F-3): sc-390587. COLO 320DM (A), SW480 (B) 和 HTC-116 (C) 全细胞裂解液中 glypican-3 表达的 Western 印迹分析.

glypican-3 抗体 (F-3): sc-390587

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说明书
  • glypican-3 抗体 F-3 是小鼠单克隆 IgM κ，glypican-3抗体, 在6篇文献中引用，规格为200 µg/ml
  • 与human的48-74氨基酸残基的glypican-3
  • 推荐用于 mouse, rat 和 human 来源的glypican-3 WB, IP, IF 和 ELISA检测; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取glypican-3 (F-3): sc-390587的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成glypican-3 抗体 (F-3)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

此外 glypican 抗体包括 glypican-1, glypican-2, glypican-3, glypican-5 和 glypican-4

glypican-3抗体（F-3）是一种IgM κ小鼠单克隆glypican-3抗体（也称为GPC3抗体或glypican 3抗体），可通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）和酶联免疫吸附测定法（ELISA）检测小鼠、大鼠和人类来源的glypican-3蛋白。glypican-3抗体（F-3）既可作为非偶联抗glypican-3抗体形式提供，也有多种偶联形式的抗glypican-3抗体可供选择，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。Glypican-3（GPC3）是一种整体膜蛋白，在Simpson-Golabi-Behmel综合征（SGBS）中发生突变。SGBS的特征是出生前和出生后的过度生长，是一种隐性X连锁疾病。glypican-3在大鼠中也称为OCI-5，是硫酸乙酰肝素蛋白多糖的glypican家族成员，通过糖基磷脂酰肌醇（GPI）锚定连接到细胞膜上。在胚胎中胚层肺、肝和肾组织中检测到glypican-3的表达。glypican-3被认为通过与胰岛素样生长因子II（IGF-II）或成纤维细胞生长因子2（FGF-2）等生长因子的相互作用来调节组织和器官的生长。glypican-3可以通过多种方式下调，包括启动子超甲基化或特定转录因子的抑制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

glypican-3 抗体 (F-3) 参考文献:

  1. 卵巢癌细胞系中 GPC3 基因的频繁沉默。  |  Lin, H., et al. 1999. Cancer Res. 59: 807-10. PMID: 10029067
  2. 缺失 Glypican-3 的小鼠表现出发育过度和辛普森-戈拉比-贝梅尔综合征的一些典型异常。  |  Cano-Gauci, DF., et al. 1999. J Cell Biol. 146: 255-64. PMID: 10402475
  3. 在恶性间皮瘤中，X 连锁隐性过度生长基因 GPC3 的表达被沉默。  |  Murthy, SS., et al. 2000. Oncogene. 19: 410-6. PMID: 10656689
  4. 结肠直肠癌中，Glypican-3免疫染色增强与存活期延长有关。  |  Al-Maghrabi, J., et al. 2022. Pol J Pathol. 73: 14-20. PMID: 35848476
  5. 糖蛋白-3的频繁表达是骨肉瘤的一种有前景的新治疗靶点。  |  Nie, JH., et al. 2022. Cancer Sci. 113: 3618-3632. PMID: 35946078
  6. 血清糖蛋白-3用于预测肝细胞癌患者的生存率。  |  Nicolosi, A., et al. 2022. Minerva Gastroenterol (Torino). 68: 378-386. PMID: 36222678
  7. 通过预先降解多余的纤维化胶原，增强肝细胞癌与肝纤维化的糖蛋白-3靶向识别。  |  Zhuo, J., et al. 2023. Acta Biomater. 158: 435-448. PMID: 36603729
  8. HIF-1α介导的肝细胞癌缺氧诱导肿瘤进展中糖蛋白-3分泌的作用和机制。  |  Wang, P., et al. 2024. Cell Signal. 114: 111007. PMID: 38081444
  9. 糖蛋白基因 GPC3 的突变会导致辛普森-戈拉比-贝梅尔生长过度综合征。  |  Pilia, G., et al. 1996. Nat Genet. 12: 241-7. PMID: 8589713
  10. OCI-5/rat glypican-3 与成纤维细胞生长因子-2 结合，但不与胰岛素样生长因子-2 结合。  |  Song, HH., et al. 1997. J Biol Chem. 272: 7574-7. PMID: 9065409
  11. 肠道形态发生过程中 OCI-5/glypican 3 的表达：肠上皮细胞中细胞形状的调控。  |  Li, M., et al. 1997. Exp Cell Res. 235: 3-12. PMID: 9281346
  12. OCI-5/GPC3是一种由辛普森-戈拉比-贝梅尔生长过快综合征基因突变所编码的糖蛋白，它能以细胞系特异性的方式诱导细胞凋亡。  |  Gonzalez, AD., et al. 1998. J Cell Biol. 141: 1407-14. PMID: 9628896

订购信息

产品名称产品编号规格价格数量收藏夹

glypican-3 抗体 (F-3)

sc-390587
200 µg/ml
RMB2377.00

glypican-3 抗体 (F-3) AC

sc-390587 AC
500 µg/ml, 25% agarose
RMB3129.00

glypican-3 抗体 (F-3) Alexa Fluor® 488

sc-390587 AF488
200 µg/ml
RMB2685.00

glypican-3 抗体 (F-3) Alexa Fluor® 647

sc-390587 AF647
200 µg/ml
RMB2685.00

glypican-3 抗体 (F-3) FITC

sc-390587 FITC
200 µg/ml
RMB2482.00

glypican-3 抗体 (F-3) HRP

sc-390587 HRP
200 µg/ml
RMB2377.00

glypican-3 (F-3) 中和勝肽

sc-390587 P
100 µg/0.5 ml
RMB511.00

glypican-3 抗体 (F-3) PE

sc-390587 PE
200 µg/ml
RMB2580.00

What secondary would you recommend for western blot since it is an IgM monoclonal? Most secondary's are to IgG and I not 100% sure they will cross-react to IgM.

Asked by: Mars
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-03-02

Is the glypican-3 Antibody (F-3): sc-390587 (clone IG12) available in conjugation with Alexa 405 ?

Asked by: Zhouhong
Thank you for your question. glypican-3 Antibody (F-3): sc-390587 is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM. We do not provide it conjugated to AF405.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What application is the blocking peptide sc-390587 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Works for immunofluorescence on mouse cellsUsed on mouse ovarian epithelial cells at 1/100 overnight at 4C then 1/500 goat α-mouse secondary for 1H at room temp. Decent signal to noise ratio.
Date published: 2017-03-24
Rated 5 out of 5 by from Nice dark western blotWe used this antibody for western blot and got a nice band for the correct molecular weight
Date published: 2017-01-13
Rated 5 out of 5 by from Produced positive Western blot data of glypicanProduced positive Western blot data of glypican-3 expression in COLO320DM, SW480 and HTC 116 whole cell lysates. -SCBT QC
Date published: 2013-08-09
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glypican-3 抗体 (F-3) is rated 4.7 out of 5 by 3.
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