Date published: 2026-10-24

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GFP Antibody (C-2) - Western Blotting - Image 288018
GFP 抗体 (C-2): sc-390394. 重组 GFP 融合蛋白的 Western 印迹分析.
GFP Antibody (C-2) - Western Blotting - Image 288018
GFP 抗体 (C-2): sc-390394. 重组 GFP 融合蛋白的 Western 印迹分析.
GFP 抗体 (C-2): sc-390394. 重组 GFP 融合蛋白的 Western 印迹分析.

GFP 抗体 (C-2): sc-390394

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寫評論提問
说明书
  • GFP抗体(C-2)是小鼠单克隆IgG2b κ, 在40篇文献中引用，规格为200 µg/ml
  • 特定于Aequorea victoria起源的GFP内部区域中氨基酸167-182之间的表位图谱
  • GFP 抗体 (C-2) 推荐用于 WB, IP, IF 和 ELISA，检测 GFP and GFP mutant fusion proteins
  • 抗GFP抗体（C-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是GFP Antibody (C-2) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与GFP Antibody (C-2) 打包提供（请参阅下面的订购信息）。
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研究信息

関連項目

GFP抗体（C-2）是一种IgG2b κ小鼠单克隆GFP抗体，可通过WB、IP、IF和ELISA检测GFP和GFP突变体融合蛋白。GFP抗体（C-2）有非偶联抗GFP抗体形式，以及多种偶联形式的抗GFP抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。绿色荧光蛋白（GFP）最初被鉴定为一种参与水母Aequorea victoria生物发光的蛋白质。当在原核细胞中表达时，GFP cDNA会产生荧光产物，不需要外源底物或辅因子，这使得GFP成为监测基因表达和体内蛋白质定位的有用工具。已经开发了几种GFP突变体，包括EGFP，其荧光比野生型GFP更强。它们的激发最大值转移更适合FACS和荧光显微镜以及双标记应用。GFP广泛用于表达载体作为融合蛋白标签，允许表达和监测融合GFP的异源蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GFP 抗体 (C-2) 参考文献:

  1. 用于蛋白质和细胞选择性光标记的可光激活 GFP。  |  Patterson, GH. and Lippincott-Schwartz, J. 2002. Science. 297: 1873-7. PMID: 12228718
  2. 通过多路复用抗体和基于 GFP 的易位检测分析多种信号通路的活动。  |  Henriksen, U., et al. 2008. Comb Chem High Throughput Screen. 11: 537-44. PMID: 18694390
  3. GFP：GFP-纳米抗体复合物的结构和热力学分析。  |  Kubala, MH., et al. 2010. Protein Sci. 19: 2389-401. PMID: 20945358
  4. 通过假单胞菌中基于 GFP 的转座子鉴定对甲酚降解途径及其在菌落中的优势表达。  |  Cho, AR., et al. 2011. J Microbiol Biotechnol. 21: 1179-83. PMID: 22127130
  5. 通过改变3'区域核苷酸序列来控制GFP的总表达。  |  Lee, SJ., et al. 2013. Microb Cell Fact. 12: 68. PMID: 23834827
  6. 洞察GFP双突变体T203V/S205A的慢质子转移结构和机制  |  Wineman-Fisher, V., et al. 2014. Phys Chem Chem Phys. 16: 11196-208. PMID: 24776960
  7. 利用高含量成像技术筛选表皮生长因子受体信号转导调节剂的 GFP-Grb2 转座试验  |  Petschnigg, J. and Ketteler, R. 2017. Bio Protoc. 7: PMID: 28936468
  8. 细胞外囊泡的 GFP 标记，用于快速工艺开发。  |  Carrillo Sanchez, B., et al. 2022. Biotechnol J. 17: e2100583. PMID: 35332662
  9. 用GFP-Trap微珠测定蛋白质-蛋白质相互作用。  |  Yilmazer, I., et al. 2023. Methods Mol Biol. 2564: 317-323. PMID: 36107351
  10. 转染 GFP 改变前列腺癌细胞的蛋白质表达模式： 蛋白质组学研究  |  Yanar, S., et al. 2024. J Fluoresc.. PMID: 38502405

订购信息

产品名称产品编号规格价格数量收藏夹

GFP 抗体 (C-2)

sc-390394
200 µg/ml
CNY2422.00

GFP (C-2): m-IgG Fc BP-HRP 套装

sc-526153
200 µg Ab; 10 µg BP
CNY2715.00

GFP (C-2): m-IgG2b BP-HRP 套装

sc-549685
200 µg Ab; 10 µg BP
CNY2715.00

GFP 抗体 (C-2) AC

sc-390394 AC
500 µg/ml, 25% agarose
CNY3189.00

GFP 抗体 (C-2) HRP

sc-390394 HRP
200 µg/ml
CNY2422.00

GFP 抗体 (C-2) FITC

sc-390394 FITC
200 µg/ml
CNY2527.00

GFP 抗体 (C-2) PE

sc-390394 PE
200 µg/ml
CNY2625.00

GFP 抗体 (C-2) Alexa Fluor® 488

sc-390394 AF488
200 µg/ml
CNY2738.00

GFP 抗体 (C-2) Alexa Fluor® 546

sc-390394 AF546
200 µg/ml
CNY2738.00

GFP 抗体 (C-2) Alexa Fluor® 594

sc-390394 AF594
200 µg/ml
CNY2738.00

GFP 抗体 (C-2) Alexa Fluor® 647

sc-390394 AF647
200 µg/ml
CNY2738.00

GFP 抗体 (C-2) Alexa Fluor® 680

sc-390394 AF680
200 µg/ml
CNY2738.00

GFP 抗体 (C-2) Alexa Fluor® 790

sc-390394 AF790
200 µg/ml
CNY2738.00

GFP (C-2) 中和勝肽

sc-390394 P
100 µg/0.5 ml
CNY519.00

I’m interested in using your GFP Antibody (C-2), sc-390394, for detecting superfolder GFP in my experiments. Could you please confirm whether this antibody is known to recognize sfGFP, or if the epitope is conserved and accessible in this variant?

Asked by: Carlos
Thank you for your question. sc-390394 GFP (C-2) is reactive with GFP and GFP mutant fusion proteins.Here is a link for more information on the protein : https://www.uniprot.org/uniprotkb/P42212/entry
Answered by: BlakeJ
Date published: 2025-08-06

We received a new lot of this antibody and it is not working (whereas a previous lot worked fine). Is it possible to get a replacement lot for free?

Asked by: sammi
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2026-02-05

What secondary is best to use with GFP Antibody (C-2): sc-390394?

Asked by: AbPolly
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with our GFP Antibody (C-2): sc-390394, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2024-12-17
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Rated 5 out of 5 by roberth from GFP antibodyThis antibody works great for western blotting using traditional approaches.
Date published: 2020-07-27
Rated 4 out of 5 by Mao123 from Good in western blotting in overexpressing taggedDilution 1:1000 Western blotting in CFBE lysate celle overexpressing a protein wity an YFP tag Good signal at the expected molecular weight
Date published: 2019-12-11
Rated 1 out of 5 by SNaphade from Did not work for IHCTested on mouse tissue..... did not work for IHC. I simultaneously tested sc-9996 and sc-81045, both gave excellent signal for the same sample. For IHC application, I would recommend these two antibodies.
Date published: 2018-06-13
Rated 4 out of 5 by DGhopkins from Works for staining but also stains tdtomatoThe antibody works well for IF staining but also detects tdtomato so you may detect other RFP variants.
Date published: 2018-05-18
Rated 4 out of 5 by sharmilee from Worked well in the detection of histone -egfpThe antibody works best in immuno. However it required optimisation to detect a single specific band for fusion protein .
Date published: 2018-04-09
Rated 5 out of 5 by sweetums from good GFP detectionAntibody produces clean results. It has been published for multiple applications and is a reliable monoclonal antibody.
Date published: 2017-01-13
Rated 5 out of 5 by lele1121 from Excellent antibody!It's an excellent antibody that I got a good result when I used this antibody for WB detection of GFP fusion protein
Date published: 2016-12-31
Rated 5 out of 5 by ChiuYueh from React well with transgenic plant protein sample.It is a free trial sample. It works better than the one I purchased anti-GFP.
Date published: 2016-12-13
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GFP 抗体 (C-2) is rated 4.1 out of 5 by 10.
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