Date published: 2025-9-7
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GFAP Antibody (GA-5) - Western Blotting - Image 376326
GFAP 抗体 (GA-5): sc-58766. 小鼠大脑 (A) 和大鼠小脑 (B) 组织提取物中 GFAP 表达的 Western 印迹分析.
GFAP 抗体 (GA-5): sc-58766. 小鼠大脑 (A) 和大鼠小脑 (B) 组织提取物中 GFAP 表达的 Western 印迹分析.

GFAP 抗体 (GA-5): sc-58766

4.9(8)
寫評論提問

说明书
  • GFAP抗体(GA-5)是小鼠单克隆IgG1 κ, 在34篇文献中引用，规格为200 µg/ml
  • 针对GFAP的，从porcine来源的脊髓中分离
  • GFAP 抗体 (GA-5) 推荐用于WB, IP, IF 和 IHC(P) ，检测 mouse, rat, human 和 avian来源的GFAP; 也和以下物种反应，包括： 和 bovine, porcine and feline, rabbit; 可能与 astrocytes, ependymal cells and corresponding tumors产生交联反应; 不会与 other intermediate filaments产生交联反应
  • 抗GFAP抗体（GA-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GFAP (GA-5): sc-58766的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 GFAP 抗体 (GA-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GFAP 抗体 (GA-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

GFAP抗体（GA-5）是一种小鼠单克隆IgG1 kappa轻链抗体，专门针对酸性成纤维细胞粘蛋白（GFAP），这种关键的中间丝状蛋白对于中枢神经系统星形胶质细胞的完整结构和功能至关重要。GFAP，也称为ALXDRD，具有独特的三部分结构，包括氨基端头域、丝状体组装所必需的中央α螺旋棒状域以及介导与其他细胞蛋白和细胞器相互作用的羧基尾域。这种结构配置对于维持星形胶质细胞的弹性和适应性至关重要，使其能够有效应对神经损伤并保持大脑和脊髓内的稳态。GFAP单克隆抗体（GA-5）是针对从猪脊髓中分离的GFAP制备的，具有广泛的物种反应性，因此GFAP（GA-5）抗体适用于通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）以及石蜡包埋切片（IHCP）和冷冻切片免疫组织化学等方法检测小鼠、大鼠、人、禽、牛、猪、猫和兔组织中的GFAP。建议研究星形胶质细胞增生症（astrogliosis）的研究人员使用GFAP（GA-5）单克隆抗体，该过程的特点是GFAP表达增加，以应对机械创伤、艾滋病相关性痴呆、朊病毒感染和炎症性脱髓鞘疾病等状况，从而为各种中枢神经系统疾病的病理生理学提供重要见解。抗GFAP抗体（GA-5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种Alexa Fluor®变体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

GFAP 抗体 (GA-5)

sc-58766
200 µg/ml
$316.00

GFAP (GA-5): m-IgG Fc BP-HRP 套装

sc-526913
200 µg Ab; 10 µg BP
$354.00

GFAP (GA-5): m-IgG1 BP-HRP 套装

sc-532286
200 µg Ab; 20 µg BP
$354.00

GFAP 抗体 (GA-5) AC

sc-58766 AC
500 µg/ml, 25% agarose
$416.00

GFAP 抗体 (GA-5) HRP

sc-58766 HRP
200 µg/ml
$316.00

GFAP 抗体 (GA-5) FITC

sc-58766 FITC
200 µg/ml
$330.00

GFAP 抗体 (GA-5) PE

sc-58766 PE
200 µg/ml
$343.00

GFAP 抗体 (GA-5) Alexa Fluor® 488

sc-58766 AF488
200 µg/ml
$357.00

GFAP 抗体 (GA-5) Alexa Fluor® 546

sc-58766 AF546
200 µg/ml
$357.00

GFAP 抗体 (GA-5) Alexa Fluor® 594

sc-58766 AF594
200 µg/ml
$357.00

GFAP 抗体 (GA-5) Alexa Fluor® 647

sc-58766 AF647
200 µg/ml
$357.00

GFAP 抗体 (GA-5) Alexa Fluor® 680

sc-58766 AF680
200 µg/ml
$357.00

GFAP 抗体 (GA-5) Alexa Fluor® 790

sc-58766 AF790
200 µg/ml
$357.00

200 µg/ml と表記されていますが、抗体がこの濃度で1ml 入っているという意味でしょうか。

Asked by: Anonymous
ご質問ありがとうございます。はい、200 µg/mlとは体積が1ml、質量が200μｇということです。 Thank you for your question. Yes, 200 µg/ml means 1 ml in volume and 200 µg in mass.
Answered by: Jiajun Z
Date published: 2024-09-11

Do you know which site is the epitope detected by this antibody?

Asked by: nois
Thank you for your question. GFAP Antibody (GA-5): sc-58766 was raised against GFAP isolated from spinal cord of porcine origin. The antibody's exact epitope has not been determined yet.
Answered by: Tech Support Europe
Date published: 2023-03-04

I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 15
Date published: 2024-04-04
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Rated 5 out of 5 by from BEST ONEMethods: Western blotting and Immunohistochemistry Sample: mouse brain lysate and section Sensitivity and specificity were both good
Date published: 2021-12-01
Rated 5 out of 5 by from signal acrossGot a good band in wb in rat cerebellum tissue extracts, we obtained specific band is very clear
Date published: 2017-05-20
Rated 5 out of 5 by from Superb data !!Great product for both western blot and IHC staining applications
Date published: 2017-02-05
Rated 5 out of 5 by from Fantastic choiceGreat antibody that produces a robust signal. Recommended for GFAP detection in a broad number of species for multiple applications.
Date published: 2017-01-05
Rated 5 out of 5 by from Successfully immunoprecipitated GFAP fromSuccessfully immunoprecipitated GFAP from rat brain tissue extract. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Worked well at 1:200 dilution for WB withWorked well at 1:200 dilution for WB with murine brain samples. -SCBT Publication Review
Date published: 2015-03-21
Rated 5 out of 5 by from Positive results/clean blot with WesternPositive results/clean blot with Western blot analysis of GFAP expression in rat brain tissue extract. -SCBT QC
Date published: 2015-01-08
Rated 4 out of 5 by from Robust signal observed in human GFAP transfectedRobust signal observed in human GFAP transfected 293 whole cell lysates. -SCBT QC
Date published: 2013-06-24
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GFAP 抗体 (GA-5) is rated 4.9 out of 5 by 8.
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