Date published: 2025-9-7
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GFAP Antibody (2E1) - Western Blotting - Image 373530
GFAP 抗体 (2E1): sc-33673. C6 (A), U-87 MG (B), SK-N-SH (C) 和 U-251-MG (D) 全细胞裂解液中 GFAP 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
GFAP 抗体 (2E1): sc-33673. C6 (A), U-87 MG (B), SK-N-SH (C) 和 U-251-MG (D) 全细胞裂解液中 GFAP 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

GFAP 抗体 (2E1): sc-33673

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说明书
  • GFAP 抗体 2E1 是小鼠单克隆 IgG2b κ，GFAP抗体, 在357篇文献中引用，规格为200 µg/ml
  • 针对脊髓弦均质物的bovine来源
  • GFAP 抗体 (2E1) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 GFAP; 也和以下物种反应，包括： bovine
  • 抗GFAP抗体（2E1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GFAP (2E1): sc-33673的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 GFAP 抗体 (2E1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GFAP 抗体 (2E1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

GFAP抗体（2E1）是一种小鼠单克隆IgG2b kappa轻链抗体，用于检测胶质纤维酸性蛋白（GFAP），这是一种主要在中枢神经系统星形胶质细胞中表达的重要中间丝蛋白。抗GFAP抗体（2E1）针对牛脊髓匀浆制备，在多种物种（包括小鼠、大鼠、人类和牛）中均表现出强烈的反应性，使其成为适用于多种应用的通用工具，如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）。GFAP，也称为ALXDRD，在维持星形胶质细胞的完整结构和功能方面发挥着至关重要的作用，而星形胶质细胞对于支持神经元网络、调节血脑屏障和促进突触传递至关重要。此外，GFAP是星形胶质细胞增生过程不可或缺的，星形胶质细胞通过增加GFAP的表达来应对神经损伤和疾病，从而帮助组织修复和疤痕形成。这使得GFAP成为研究多种神经系统疾病的重要生物标记物，包括机械性创伤、艾滋病相关性痴呆、朊病毒感染和炎症性脱髓鞘疾病。通过靶向GFAP，GFAP (2E1) 单克隆抗体使研究人员能够研究星形胶质细胞的生物学，监测疾病模型中的胶质反应，并识别中枢神经系统中的良性胶质细胞和肿瘤性胶质细胞。GFAP (2E1)抗体有非结合型和多种结合型，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种Alexa Fluor®结合物，具有灵活性和可靠性，能够满足各种实验需求，促进神经科学研究的发展，加深对神经胶质细胞功能和病理的认识。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GFAP 抗体 (2E1) 参考文献:

  1. 分析衰老过程中和阿尔茨海默氏症患者内耳皮层中的神经胶质酸性纤维蛋白。  |  Porchet, R., et al. 2003. Proteomics. 3: 1476-85. PMID: 12923773
  2. 人类星形细胞瘤中胶质纤维酸性蛋白和波形蛋白型中间丝的共同表达。  |  Herpers, MJ., et al. 1986. Acta Neuropathol. 70: 333-9. PMID: 3020864
  3. 中间丝多肽在人类胎儿和成人组织中的共表达。  |  Van Muijen, GN., et al. 1987. Lab Invest. 57: 359-69. PMID: 3669613
  4. 神经胶质纤维酸性蛋白的免疫组化：基础和应用方面的考虑。  |  McLendon, RE. and Bigner, DD. 1994. Brain Pathol. 4: 221-8. PMID: 7952263
  5. GFAP 和星形胶质细胞增生  |  Eng, LF. and Ghirnikar, RS. 1994. Brain Pathol. 4: 229-37. PMID: 7952264
  6. 胶质纤维酸性蛋白：动态特性和磷酸化调节。  |  Inagaki, M., et al. 1994. Brain Pathol. 4: 239-43. PMID: 7952265
  7. GFAP 基因的结构和转录调控。  |  Brenner, M. 1994. Brain Pathol. 4: 245-57. PMID: 7952266
  8. 神经胶质纤维酸性蛋白：激素, 细胞因子和生长因子的调节。  |  Laping, NJ., et al. 1994. Brain Pathol. 4: 259-75. PMID: 7952267
  9. 人体皮质中的胶质纤维酸性蛋白（GFAP）免疫组化：使用不同抗血清的定量研究。  |  Halliday, GM., et al. 1996. Neurosci Lett. 209: 29-32. PMID: 8734902

订购信息

产品名称产品编号规格价格数量收藏夹

GFAP 抗体 (2E1)

sc-33673
200 µg/ml
$316.00

GFAP (2E1): m-IgG Fc BP-HRP 套装

sc-528485
200 µg Ab; 10 µg BP
$354.00

GFAP (2E1): m-IgGκ BP-HRP 套装

sc-520876
200 µg Ab, 40 µg BP
$354.00

GFAP (2E1): m-IgG2b BP-HRP 套装

sc-550042
200 µg Ab; 10 µg BP
$354.00

GFAP 抗体 (2E1) AC

sc-33673 AC
500 µg/ml, 25% agarose
$416.00

GFAP 抗体 (2E1) HRP

sc-33673 HRP
200 µg/ml
$316.00

GFAP 抗体 (2E1) FITC

sc-33673 FITC
200 µg/ml
$330.00

GFAP 抗体 (2E1) PE

sc-33673 PE
200 µg/ml
$343.00

GFAP 抗体 (2E1) Alexa Fluor® 488

sc-33673 AF488
200 µg/ml
$357.00

GFAP 抗体 (2E1) Alexa Fluor® 546

sc-33673 AF546
200 µg/ml
$357.00

GFAP 抗体 (2E1) Alexa Fluor® 594

sc-33673 AF594
200 µg/ml
$357.00

GFAP 抗体 (2E1) Alexa Fluor® 647

sc-33673 AF647
200 µg/ml
$357.00

GFAP 抗体 (2E1) Alexa Fluor® 680

sc-33673 AF680
200 µg/ml
$357.00

GFAP 抗体 (2E1) Alexa Fluor® 790

sc-33673 AF790
200 µg/ml
$357.00

Where is this antibody manufactured and shipped from? I'm located in Canada and my institution has banned importing supplies from the United States. Is this antibody manufactured anywhere else?

Asked by: Sierra
Thank you for your question. For assistance please contact technical service. Please call 800-457-3801or email [email protected].
Answered by: BlakeJ
Date published: 2025-05-14

Does GFAP protein stain well in paraformaldehyde-fixed frozen sections of rat brain tissue?"

Asked by: colossus
Thank you for your question. GFAP Antibody (2E1): sc-33673 is recommended for detection of GFAP of mouse, rat and human origin by WB, IP, IF and IHC(P), including frozen tissues, and would be suitable for your planned application.
Answered by: Tech Support Europe
Date published: 2025-04-09

I want use 'sc-33673 AF680' for IHC of mouse brain sections. Please teach me the suitable concentration and incubation conditions for it.

Asked by: NNOZ
Thank you for your question. please refer to the protocol:https: www.scbt.com/resources/protocols/immunofluorescence-cell-staining. Please contact Technical Service if you have further questions concerning this product.
Answered by: Sen Li
Date published: 2024-04-24

I would like to obtain a GFAP mouse monoclonal antibody. We are currently using another company's clone GA-5 that has been stored -80 for 2 years and seeing background along stromal fibers in feline tissue. Which product would you recommend? Free smp

Asked by: Thurma
Thank you for your question. We currently do not have any data or product citations using any of our GFAP antibodies in feline tissues. We also offer the GA-5 clone, sc-58766. Please contact our Technical Service Department for more information on how to receive free 10 ug samples of some of our monoclonal antibodies.
Answered by: Technical Support
Date published: 2019-05-16

Dose this antibody work on cerebellum, especially in case of immunohistochemistry?

Asked by: nobu1113
Thank you for your question. We have immunohistochemistry data using this antibody in cerebral cortex and brain. We are aware of a publication using this antibody in mouse cerebellum. Please refer to PMID: 27586038 for additional details about this product citation.
Answered by: Technical Support
Date published: 2019-04-22

does the antibody work on peripheral nerves

Asked by: abibelai
Thank you for your question. GFAP Antibody (2E1): sc-33673 is suitable for IF and IHC(P) and we have used the antibody to successfully detect GFAP in the central nervous system (brain and cerebral cortex). We have not tested the antibody on peripheral nerves, and are not aware of any publications using the antibody in peripheral nerves. This would have to be determined experimentally, and would be dependent on the expression of GFAP in those samples.
Answered by: Technical Support
Date published: 2017-03-09

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-33673 PE) or fluoroscein (sc-33673 FITC), Alexa Fluor® 488 (sc-33673 AF488) or Alexa Fluor® 647 (sc-33673 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2024-01-11
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Rated 5 out of 5 by from GFAP to label spinal cord astrocytes in vitroWe studied cell-type specific activation of inflammatory transcription factors in cultured cells of the spinal dorsal horn. The GFAP antibody was used to label astrocytes and worked very well in a dilution of 1:1000.
Date published: 2022-11-02
Rated 5 out of 5 by from InteresanteMuy bueno, recomendable completamente. Útil y a buen precio
Date published: 2022-08-21
Rated 5 out of 5 by from anti-GFAP antibody (SC-33673) works well in WBWestern Blot analysis in 10% SDS-PAGE of 40 μg of proteins from 2 human glial tumour cell lines lysates. Anti-GFAP Ab diluted 1:500; 4°C overnight.
Date published: 2022-07-07
Rated 4 out of 5 by from 染色（IHC）マウス脳組織に対してIHC目的に使用いたしました。1:100で使用、 良好な染色が得られておりました。
Date published: 2021-12-10
Rated 5 out of 5 by from Remarkable features!This antibody works great in western blot analysis in Rat Brain Astrocytes.
Date published: 2021-07-20
Rated 5 out of 5 by from Excellent for WB and IFIt works very well for WB and IF in mouse spinal cord! Really great!
Date published: 2021-04-06
Rated 5 out of 5 by from ExcellentI tried this antibody on organoids and it worked very well.
Date published: 2020-10-13
Rated 5 out of 5 by from very good product for IHCWe got it sevral years ago, it still works very well at 1:1500 for IHC!!!
Date published: 2020-03-12
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GFAP抗体 (2E1) is rated 4.8 out of 5 by 47.
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