Date published: 2025-9-7

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GFAP 抗体 (2A5): sc-65343

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说明书
  • GFAP抗体(2A5)是小鼠单克隆IgG1 κ, 在26篇文献中引用,规格为200 µg/ml
  • 针对纯化的porcine来源脊髓spinal cord GFAP
  • 推荐用于 mouse, rat, human 和 porcine 来源的GFAP WB, IP, IF 和 IHC(P)检测
  • 关于如何获取GFAP (2A5): sc-65343免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 GFAP 抗体 (2A5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GFAP 抗体 (2A5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

GFAP 抗体(2A5)是一种小鼠单克隆 IgG1 kappa 轻链抗体,设计用于检测神经胶质纤维酸性蛋白(GFAP)。GFAP 又称 ALXDRD,在维持中枢神经系统内星形胶质细胞的结构完整性和功能性方面起着至关重要的作用。GFAP 的特点是其三方结构,包括一个氨基末端头部结构域、一个负责组装丝状结构的中央 α 螺旋杆结构域和一个促进与其他细胞蛋白质和细胞器相互作用的羧基末端尾部结构域。GFAP 的翻译后修饰,尤其是磷酸化,对于调节丝的动力学、组装和细胞对损伤的反应至关重要,因此检测这些修饰对于了解星形胶质细胞在各种病理条件下的行为非常重要。GFAP 单克隆抗体 (2A5) 是针对纯化的猪脊髓 GFAP 培育的,在包括小鼠、大鼠、人和猪在内的多个物种中都表现出强大的反应活性,使 GFAP (2A5) 抗体成为 Western 印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和石蜡包埋切片免疫组化 (IHCP) 等应用的理想工具。抗 GFAP 抗体 (2A5) 尤其适用于研究星形胶质细胞增生,这一过程的特点是 GFAP 在神经损伤(如机械性创伤)、艾滋病相关性痴呆、朊病毒感染和炎症性脱髓鞘疾病时表达增加,从而为了解中枢神经系统疾病的发病机制提供重要依据。GFAP (2A5) 单克隆抗体以非结合形式提供,是研究人员阐明 GFAP 在神经健康和疾病中的复杂作用不可或缺的试剂,有助于对支配星形胶质细胞功能和损伤反应的分子和细胞途径进行深入研究。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

GFAP 抗体 (2A5)

sc-65343
200 µg/ml
RMB2377.00

GFAP (2A5): m-IgG Fc BP-HRP 套装

sc-526953
200 µg Ab; 10 µg BP
RMB2662.00

GFAP (2A5): m-IgG1 BP-HRP 套装

sc-532326
200 µg Ab; 20 µg BP
RMB2662.00

Are there any issues with using sc-65343: GFAP (2A5) mouse monoclonal primary antibody on mouse samples?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-65343 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by from IFl labeling in mouse retinaSCBT provided us a generous sample to test before purchase. Both the 2A5 and 2E1 labeled well in frozen sections of fixed mouse retina at 5ug/ml. We are going forward with purchase of the bovine form of the ab, 2E1. Thanks
Date published: 2017-09-22
Rated 5 out of 5 by from Positive resultsGFAP Antibody (2A5) produced strong positive results by WB
Date published: 2017-01-25
Rated 4 out of 5 by from Worked well in human/mice sample for WBWorked well in human/mice sample for WB
Date published: 2015-09-16
Rated 3 out of 5 by from Published immunohistochemical analysis usingPublished immunohistochemical analysis using murine spinal cord sections (paraffin embedded). -SCBT Publication Review
Date published: 2015-04-16
Rated 4 out of 5 by from Clean immunoblot and immunohistochemicalClean immunoblot and immunohistochemical analysis in human fetal spinal cord extracts. -SCBT Publication Review
Date published: 2015-03-05
Rated 3 out of 5 by from Worked well for IF with paraformaldehydeWorked well for IF with paraformaldehyde fixed mouse neuron slices. -SCBT Publication Review
Date published: 2015-01-24
Rated 3 out of 5 by from Publishable WB data in rat brain lysatesPublishable WB data in rat brain lysates. -SCBT Publication Review
Date published: 2015-01-16
Rated 3 out of 5 by from Overblown band observed in human GFAP transfectedOverblown band observed in human GFAP transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-04-26
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GFAP 抗体 (2A5) is rated 3.6 out of 5 by 8.
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