Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
GCN5 Antibody (A-11) - Western Blotting - Image 346633
GCN5 抗体 (A-11): sc-365321. Hep G2 (A), K-562 (B) 和紫外线处理的 HeLa (C) 核提取物中 GCN5 表达的 Western 印迹分析.
GCN5 抗体 (A-11): sc-365321. Hep G2 (A), K-562 (B) 和紫外线处理的 HeLa (C) 核提取物中 GCN5 表达的 Western 印迹分析.

GCN5 抗体 (A-11): sc-365321

4.5(10)
寫評論提問

说明书
  • GCN5 抗体 A-11 是小鼠单克隆 IgG1 κ，GCN5抗体, 在32篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的GCN5的N-terminus的氨基酸363-387之间
  • GCN5 抗体 (A-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GCN5; 也和以下物种反应，包括： 和 canine and porcine
  • 抗GCN5抗体（A-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-365321 X, 200 µg/0.1 ml)
  • 关于如何获取GCN5 (A-11): sc-365321的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 GCN5 抗体 (A-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GCN5 抗体 (A-11) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

GCN5抗体（A-11）是一种IgG1κ型小鼠单克隆抗体，可以检测到来自小鼠、大鼠和人类的GCN5蛋白，通过WB、IP、IF、IHC（P）和ELISA方法进行检测。GCN5抗体（A-11）既可以是非偶联的抗GCN5抗体形式，也可以是多种偶联形式的抗GCN5抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在完整的细胞中，DNA与组蛋白和其他核蛋白紧密结合形成染色质。染色质的重塑被认为是转录调控的关键组成部分，这种重塑的主要来源是由核小体组蛋白的乙酰化引起的。组蛋白氨基末端尾部区域赖氨酸残基的乙酰化导致核小体构象的变构改变，并增加了DNA对转录因子的可及性。相反，组蛋白的去乙酰化与转录沉默有关。已经确定了几种哺乳动物蛋白作为核组蛋白乙酰酶，包括GCN5、PCAF（p300/CBP相关因子）、p300/CBP和TFIID亚基TAF II p250。哺乳动物HDAC1（也称为HD1）和HDAC2（也称为哺乳动物RPD3），均与酵母转录调节器Rpd3p相关，被确定为组蛋白去乙酰酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GCN5 抗体 (A-11) 参考文献:

  1. 抑制 GCN5 的表达或活性可减轻非小细胞肺癌中 c-MYC 的表达。  |  Mustachio, LM., et al. 2019. Am J Cancer Res. 9: 1830-1845. PMID: 31497362
  2. KAT2A/GCN5的自噬降解可通过减少TUBA/α-tubulin乙酰化促进血管平滑肌细胞的定向迁移。  |  Ouyang, C., et al. 2020. Autophagy. 16: 1753-1770. PMID: 31878840
  3. 血管活性肠肽（VIP）对女性类固醇生成的影响。  |  Espinola-Lopez, JM. and Tan, S. 2021. Biochim Biophys Acta Gene Regul Mech. 1864: 194629. PMID: 32890768
  4. 哮喘中的血管活性肠肽（VIP）。  |  Lehrer, S. and Rheinstein, PH. 2023. Chronic Dis Transl Med. 9: 263-265. PMID: 36721422
  5. 乙酰化导致的核小体结构变化  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
  6. 组蛋白乙酰化在转录因子进入核糖体 DNA 的过程中发挥了积极作用。  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
  7. 酵母的转录沉默与核小体乙酰化减少有关。  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
  8. 四膜虫组蛋白乙酰转移酶 A：酵母 Gcn5p 的同源物，将组蛋白乙酰化与基因激活联系起来。  |  Brownell, JE., et al. 1996. Cell. 84: 843-51. PMID: 8601308
  9. 一种与酵母转录调节因子 Rpd3p 相关的哺乳动物组蛋白去乙酰化酶。  |  Taunton, J., et al. 1996. Science. 272: 408-11. PMID: 8602529
  10. 一种与腺病毒癌蛋白 E1A 竞争的 p300/CBP 相关因子。  |  Yang, XJ., et al. 1996. Nature. 382: 319-24. PMID: 8684459
  11. YY1 对转录的抑制是通过与哺乳动物中酵母全局调节因子 RPD3 的同源物相互作用来介导的。  |  Yang, WM., et al. 1996. Proc Natl Acad Sci U S A. 93: 12845-50. PMID: 8917507
  12. 将小鼠组蛋白去乙酰化酶 1 鉴定为生长因子诱导基因  |  Bartl, S., et al. 1997. Mol Cell Biol. 17: 5033-43. PMID: 9271381

订购信息

产品名称产品编号规格价格数量收藏夹

GCN5 抗体 (A-11)

sc-365321
200 µg/ml
RMB2377.00

GCN5 (A-11): m-IgG Fc BP-HRP 套装

sc-529349
200 µg Ab; 10 µg BP
RMB2662.00

GCN5 (A-11): m-IgGκ BP-HRP 套装

sc-522205
200 µg Ab, 40 µg BP
RMB2662.00

GCN5 (A-11): m-IgG1 BP-HRP 套装

sc-543471
200 µg Ab; 20 µg BP
RMB2662.00

GCN5 抗体 (A-11) X

sc-365321 X
200 µg/0.1 ml
RMB2377.00

GCN5 抗体 (A-11) AC

sc-365321 AC
500 µg/ml, 25% agarose
RMB3129.00

GCN5 抗体 (A-11) HRP

sc-365321 HRP
200 µg/ml
RMB2377.00

GCN5 抗体 (A-11) FITC

sc-365321 FITC
200 µg/ml
RMB2482.00

GCN5 抗体 (A-11) PE

sc-365321 PE
200 µg/ml
RMB2580.00

GCN5 抗体 (A-11) Alexa Fluor® 488

sc-365321 AF488
200 µg/ml
RMB2685.00

GCN5 抗体 (A-11) Alexa Fluor® 546

sc-365321 AF546
200 µg/ml
RMB2685.00

GCN5 抗体 (A-11) Alexa Fluor® 594

sc-365321 AF594
200 µg/ml
RMB2685.00

GCN5 抗体 (A-11) Alexa Fluor® 647

sc-365321 AF647
200 µg/ml
RMB2685.00

GCN5 抗体 (A-11) Alexa Fluor® 680

sc-365321 AF680
200 µg/ml
RMB2685.00

GCN5 抗体 (A-11) Alexa Fluor® 790

sc-365321 AF790
200 µg/ml
RMB2685.00

GCN5 (A-11) 中和勝肽

sc-365321 P
100 µg/0.5 ml
RMB511.00

Hi, Will this Antibody detect GCN5 of Pichia pastoris??

Asked by: aditi gupta
Thank you for your question. GCN5 Antibody (A-11): sc-365321 is recommended for detection of GCN5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and porcine. We currently have no data indicating whether or not the antibody is specific against GCN5 of Pichia pastoris. Please contact out technical service directly to arrange for a sample of this antibody so you can try out whether it would be suitable for your application.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-24
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_365321, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 108ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great for western blottingStrong band and specificity was confirmed with siRNA
Date published: 2024-05-28
Rated 5 out of 5 by from Good for WB and worked for ChIPIt is good for WB with human whole cell lysate. It worked for ChIP with human cells but not great.
Date published: 2020-07-21
Rated 3 out of 5 by from It works for western blot.It detected endogenous GCN5 in HEK 293T cell lysates (1:500 dilution in 5% milk in TBS-0.1% Tween, overnight at 4C).
Date published: 2018-04-09
Rated 5 out of 5 by from very goodwe use this antibody just for pre-test and got satisfied band
Date published: 2017-06-23
Rated 4 out of 5 by from a good antibodythe GCN5 antibody 1：500 for western blot,and has a specific band.
Date published: 2017-06-12
Rated 5 out of 5 by from has been great.GCN5 antibody worked well for wb detection, It is a very specific antibody.
Date published: 2017-05-20
Rated 5 out of 5 by from workable and specificI knockdown GCN5 by siRNA in Hela cells and detect this antibody by western blot, a specific band could be detect and siRNA knockdown could confirm this band is GCN5.
Date published: 2017-05-10
Rated 5 out of 5 by from Excellent antibodyGCN5 Antibody (A-11) has performed well in WB, IF and IHC(P) applications. An excellent choice for GCN5.
Date published: 2017-01-05
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_365321, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 17ms
  • REVIEWS, PRODUCT
GCN5抗体 (A-11) is rated 4.5 out of 5 by 10.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_365321, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 114ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。