Date published: 2025-9-6
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GATA-1 Antibody (N6) - Immunohistochemistry - Image 1179
GATA-1 抗体 (N6): sc-265. 福尔马林固定, 石蜡包埋的小鼠睾丸组织的免疫过氧化物酶染色, 显示 Sertoli 细胞的核染色.
GATA-1 Antibody (N6) - Immunohistochemistry - Image 1179
GATA-1 抗体 (N6): sc-265. 福尔马林固定, 石蜡包埋的小鼠睾丸组织的免疫过氧化物酶染色, 显示 Sertoli 细胞的核染色.
GATA-1 Antibody (N6) - Western Blotting - Image 1173
GATA-1 抗体 (N6): sc-265. 对 NIH/3T3 核提取物中 GATA-1 的表达进行 Western 印迹分析.
GATA-1 抗体 (N6): sc-265. 福尔马林固定, 石蜡包埋的小鼠睾丸组织的免疫过氧化物酶染色, 显示 Sertoli 细胞的核染色.

GATA-1 抗体 (N6): sc-265

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寫評論提問

说明书
  • GATA-1 抗体 N6 是大鼠单克隆 IgG2a，GATA-1抗体, 在307篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的重组GATA-1
  • GATA-1 抗体 (N6) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 GATA-1
  • 抗GATA-1抗体（N6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-265 X, 200 µg/0.1 ml)
  • 关于如何获取GATA-1 (N6): sc-265的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成GATA-1 抗体 (N6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 GATA 抗体 包括 GATA-1, GATA-2, GATA-3, GATA-4, GATA-5和GATA-6

GATA-1抗体（N6）是一种鼠单克隆IgG2a抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHC）检测小鼠、大鼠和人类来源的GATA-1。GATA-1 (N6) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合型。GATA-1 在红细胞生成中起着至关重要的作用，因为 GATA-1 对红细胞特异性基因的转录调控至关重要，从而影响红细胞发育。GATA-1的功能对于正常的造血至关重要，GATA-1失调可导致各种血液疾病，包括贫血和其他与红细胞系相关的疾病。GATA-1的特征在于其保守的锌指DNA结合结构域，该结构域可与WGATAR共识序列结合，而后者对于基因调控至关重要。GATA-1与其他GATA家族成员（如GATA-2和GATA-3）相互作用，这些成员具有更广泛的调节能力，表明造血系统内存在复杂的转录调控网络。了解GATA-1的功能和相互作用对于阐明红细胞发育的机制和相关疾病的潜在治疗靶点至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GATA-1 抗体 (N6) 参考文献:

  1. 人类转录因子 GATA-2。内皮细胞中前内皮素-1 基因表达调控的证据。  |  Dorfman, DM., et al. 1992. J Biol Chem. 267: 1279-85. PMID: 1370462
  2. GATA-1 在 EGF 通过 ERK2 介导的人肝癌细胞核苷酸切除修复活性和 ERCC1 表达中至关重要。  |  Andrieux, LO., et al. 2007. Cancer Res. 67: 2114-23. PMID: 17332341
  3. 独立于 GATA-1 的转录抑制之友：GATA-1 功能的一种新模式。  |  Johnson, KD., et al. 2007. Blood. 109: 5230-3. PMID: 17339418
  4. LMO2基因的一个新转录本LMO2-c受GATA-1和PU.1调控，编码LMO2的拮抗剂。  |  Wang, Q., et al. 2007. Leukemia. 21: 1015-25. PMID: 17361224
  5. GATA-1 的自我结合控制着体内红细胞的发育。  |  Shimizu, R., et al. 2007. J Biol Chem. 282: 15862-71. PMID: 17374603
  6. 小鼠和人类 T 淋巴细胞 GATA-3 因子通过人类 T 细胞受体 delta 基因增强子中的顺式调节元件介导转录。  |  Ko, LJ., et al. 1991. Mol Cell Biol. 11: 2778-84. PMID: 2017177
  7. HDAC1是GATA-1转录活性、整体染色质占用和造血所必需的。  |  Yan, B., et al. 2021. Nucleic Acids Res. 49: 9783-9798. PMID: 34450641
  8. GATA-1 基因突变改变了成年小鼠睾丸的精原细胞期和类固醇生成。  |  Gonçalves, MFF., et al. 2022. Mol Cell Endocrinol. 542: 111519. PMID: 34843900
  9. GATA-1通过上调高海拔性多血症CD34+细胞中miR-451a和miR-210-3p的表达促进红细胞分化。  |  Liu, F., et al. 2023. High Alt Med Biol. 24: 59-67. PMID: 36749159
  10. GATA-4/5/6，在发育中的心脏和肠道中转录的三个转录因子亚家族。  |  Laverriere, AC., et al. 1994. J Biol Chem. 269: 23177-84. PMID: 8083222
  11. GATA 转录因子家族的 DNA 结合特异性。  |  Ko, LJ. and Engel, JD. 1993. Mol Cell Biol. 13: 4011-22. PMID: 8321208
  12. 人类 GATA-6 基因：结构, 染色体位置以及组织特异性和有丝分裂原反应信号对表达的调控。  |  Suzuki, E., et al. 1996. Genomics. 38: 283-90. PMID: 8975704

订购信息

产品名称产品编号规格价格数量收藏夹

GATA-1 抗体 (N6)

sc-265
200 µg/ml
RMB2377.00

GATA-1 抗体 (N6) X

sc-265 X
200 µg/0.1 ml
RMB2377.00

GATA-1 抗体 (N6) AC

sc-265 AC
500 µg/ml, 25% agarose
RMB3129.00

GATA-1 抗体 (N6) HRP

sc-265 HRP
200 µg/ml
RMB2377.00

GATA-1 抗体 (N6) FITC

sc-265 FITC
200 µg/ml
RMB2482.00

GATA-1 抗体 (N6) PE

sc-265 PE
200 µg/ml
RMB2580.00

GATA-1 抗体 (N6) Alexa Fluor® 488

sc-265 AF488
200 µg/ml
RMB2685.00

GATA-1 抗体 (N6) Alexa Fluor® 546

sc-265 AF546
200 µg/ml
RMB2685.00

GATA-1 抗体 (N6) Alexa Fluor® 594

sc-265 AF594
200 µg/ml
RMB2685.00

GATA-1 抗体 (N6) Alexa Fluor® 647

sc-265 AF647
200 µg/ml
RMB2685.00

GATA-1 抗体 (N6) Alexa Fluor® 680

sc-265 AF680
200 µg/ml
RMB2685.00

GATA-1 抗体 (N6) Alexa Fluor® 790

sc-265 AF790
200 µg/ml
RMB2685.00

Is sc-265 X also suitable for Western blotting?

Asked by: Kiki Chem
Thank you for your question. Yes, you can use sc-265 X for Western blotting. The only difference is that the "X" version of the antibody is 10x more concentrated in a buffer without gelatin (PBS + sodium azide only). Therefore, we recommend adjusting the recommended dilutions for Western blotting accordingly. For example, instead of using a 1:100 dilution, you could use a 1:1,000 dilution for Western blotting with the sc-265 X version of the antibody.
Answered by: Technical Support
Date published: 2020-05-07

Does GATA-1 (N6) sc-265 antibody recognize the full and short isoform of GATA-1?

Asked by: Ricko
Thank you for your question. GATA-1 Antibody (N6) is a rat monoclonal IgG2a raised against recombinant GATA-1 of mouse origin. The exact epitope recognized by this monoclonal antibody has not been determined. We have not specifically tested this antibody for reactivity with different isoforms of GATA-1. We recommend you refer to some of the product citations using this antibody to see if anyone else has published this information previously.
Answered by: Technical Support
Date published: 2019-12-03

Does GATA-1 (N6) sc-265 bind to N-terminal or C-terminal of GATA1? I want to detect N-terminal only. Thank you.

Asked by: Jesmine
Thank you for your question. This antibody, sc-265 GATA-1 (N6), maps to the N-terminus of GATA-1. If you have any further questions, please reach out to your local distributor. You can find their information here: https://www.scbt.com/scbt/resources/distributors
Answered by: Tech Service
Date published: 2017-12-04

What is the recommended storage condition for GATA-1 (N6): sc-265?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend storing the antibody at 4° C.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from 免疫組織化学で良好な結果免疫蛍光染色により精巣におけるセルトリ細胞のGATA-1を検出できた。 さらに、特異性も高く優れた抗体である。
Date published: 2021-01-29
Rated 4 out of 5 by from 抗体小样很给力买了一支常用抗体，赠送的小样，很慷慨，很好用。建议多搞这样的活动，有些分子不确定表达，就是试一把。这时候小样很重要。确定表达了，再买，减少浪费
Date published: 2020-06-28
Rated 5 out of 5 by from Great Images!Used it in an EMSA setting to detect GATA-1 binding to DNA and got clear data. Totaly recommended!
Date published: 2019-10-05
Rated 5 out of 5 by from IF很好用我用在小鼠卵黄膜上血细胞的免疫荧光染色，1:200稀释很好用，背景很低，阳性信号强，是一个好抗体，推荐！
Date published: 2018-10-18
Rated 4 out of 5 by from WorksFirst purchased this antibody in 2012 and 3 years later, it stopped working despite different antigen retrieval methods. Many SC antibodies work well beyond their recommended use date, but this is not one of them. So re-ordered in 2018 and it works well. We used this antibody on PFA fixed, paraffin embedded mouse testis cut at 5um sections, for immuno-fluorescent staining. We used a pH6 antigen retrieval method in a PT-Link machine or pressure cooker. NOTE: The antibody does not work on bouin fixed tissues.
Date published: 2018-06-05
Rated 5 out of 5 by from Good for WB, Co-IP, and ChIPThis antibody is good for Western blot, co-IP and ChIP using mouse cells.
Date published: 2018-05-12
Rated 5 out of 5 by from GATA-1 antibodyI used the antibody for immunofluroscence staining and the antibody worked fine
Date published: 2017-09-22
Rated 5 out of 5 by from Superb antibodyClear, specific western along with very strong ihc data
Date published: 2017-01-03
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GATA-1抗体 (N6) is rated 4.5 out of 5 by 16.
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