Date published: 2025-9-6
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GATA-1 Antibody (N1) - Western Blotting - Image 1174
GATA-1 抗体 (N1): sc-266. K-562 核提取物中 GATA-1 表达的 Western 印迹分析.
GATA-1 Antibody (N1) - Western Blotting - Image 1174
GATA-1 抗体 (N1): sc-266. K-562 核提取物中 GATA-1 表达的 Western 印迹分析.
GATA-1 Antibody (N1) - Western Blotting - Image 150240
GATA-1 抗体 (N1): sc-266. HEL 92. 1. 7 (A), MEG-01 (B) 和 TF-1 (C) 全细胞裂解液中 GATA-1 表达的 Western 印迹分析.
GATA-1 Antibody (N1) - Western Blotting - Image 391496
GATA-1 抗体 (N1) Alexa Fluor® 647: sc-266 AF647. K-562 核提取物 (A) 和 HEL 92. 1. 7 全细胞裂解液 (B) 中 GATA-1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.
GATA-1 抗体 (N1): sc-266. K-562 核提取物中 GATA-1 表达的 Western 印迹分析.

GATA-1 抗体 (N1): sc-266

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寫評論提問

说明书
  • GATA-1 抗体 N1 是大鼠单克隆 IgG2a, 在71篇文献中引用，提供 200 µg/ml
  • 针对细菌表达的小鼠GATA-1
  • 抗-GATA-1 抗体 (N1) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 GATA-1; 抑制凝胶迁移研究中的DNA结合
  • 抗GATA-1抗体（N1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-266 X, 200 µg/0.1 ml)
  • 关于如何获取GATA-1 (N1): sc-266的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成GATA-1 抗体 (N1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 GATA 抗体 包括 GATA-1, GATA-2, GATA-3, GATA-4, GATA-5和GATA-6

GATA-1抗体（N1）是一种鼠单克隆IgG2a抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）等方法检测小鼠、大鼠和人类样本中的GATA-1。GATA-1 (N1) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。GATA-1 在红细胞生成中起着至关重要的作用，因为 GATA-1 对红细胞特异性基因的转录调控至关重要，从而影响红细胞的发展。GATA-1主要位于细胞核中，通过其保守的锌指DNA结合结构域与WGATAR共识序列结合，促进红系细胞分化所需的基因表达。GATA-1的核定位功能使其能够在造血过程中精确控制基因表达，确保红细胞的正常形成和功能。GATA-1与其他GATA家族成员（如GATA-2和GATA-3）相互作用，这些成员具有更广泛的调节能力，突出了调控血细胞发育的转录因子的复杂网络。了解GATA-1的动态和相互作用对于阐明红细胞生成的机制以及相关疾病的潜在影响至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

GATA-1 抗体 (N1)

sc-266
200 µg/ml
$316.00

GATA-1 抗体 (N1) X

sc-266 X
200 µg/0.1 ml
$316.00

GATA-1 抗体 (N1) AC

sc-266 AC
500 µg/ml, 25% agarose
$416.00

GATA-1 抗体 (N1) HRP

sc-266 HRP
200 µg/ml
$316.00

GATA-1 抗体 (N1) FITC

sc-266 FITC
200 µg/ml
$330.00

GATA-1 抗体 (N1) PE

sc-266 PE
200 µg/ml
$343.00

GATA-1 抗体 (N1) Alexa Fluor® 488

sc-266 AF488
200 µg/ml
$357.00

GATA-1 抗体 (N1) Alexa Fluor® 546

sc-266 AF546
200 µg/ml
$357.00

GATA-1 抗体 (N1) Alexa Fluor® 594

sc-266 AF594
200 µg/ml
$357.00

GATA-1 抗体 (N1) Alexa Fluor® 647

sc-266 AF647
200 µg/ml
$357.00

GATA-1 抗体 (N1) Alexa Fluor® 680

sc-266 AF680
200 µg/ml
$357.00

GATA-1 抗体 (N1) Alexa Fluor® 790

sc-266 AF790
200 µg/ml
$357.00

Does GATA-1 (N1) sc-266 bind to N-terminal or C-terminal of GATA1? I want to detect C-terminal only. Thank you

Asked by: Double T
Thank you for your questin. GATA-1 Antibody (N1): sc-266 was raised against bacterially expressed murine GATA-1. No epitope mapping of this antibody has so far been performed, so we are not able to say whether it recognizes the N- or C-terminus or an internal region of the protein. Feel free to contact our Technical Service Department for more information on how to receive a free 10 µg sample of GATA-1 (N1): sc-266 if you would like to test the antibody.
Answered by: Tech Support Europe
Date published: 2020-06-29

When should the TransCruz reagent sc-266 X be used instead of the standard form sc-266?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Tech Service
Date published: 2017-01-31
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Rated 5 out of 5 by from 免疫組織化学で良好な結果免疫蛍光染色により精巣におけるセルトリ細胞のGATA-1を検出できた。 さらに、特異性も非常に高く優れた抗体である。
Date published: 2021-01-29
Rated 5 out of 5 by from Nice bandWe used this antibody for a western blot and got a very clean band
Date published: 2016-12-23
Rated 5 out of 5 by from This antibody worked well for IPThis antibody worked well for IP, published with Cos, HPCs (human) and mouse lymphoma cells. -SCBT Publication Review
Date published: 2015-04-20
Rated 5 out of 5 by from This GATAThis GATA-1 antiobody (N1) was useful in immunohistochemistry using human hematopoietic cells. -SCBT Publication Review
Date published: 2015-04-16
Rated 5 out of 5 by from Publishable data obtained using product scPublishable data obtained using product sc-266 in immunohistochemistry on formalin fixed, paraffin embedded (FFPE) mouse gonads. -SCBT Publication Review
Date published: 2015-04-06
Rated 5 out of 5 by from Works well in Western Blot using human hematopoietic, HPCs, erythroid, K-562, MEL, UT-7, Jurkat, HeLa, UT-7 5.3, U-937, HL-60 HEL and TF1 cell lines. -SCBT Publication Review
Date published: 2015-04-03
Rated 5 out of 5 by from Human MEL and umbelical vein endothelialHuman MEL and umbelical vein endothelial cells were used in ChIP application successfully and results published. -SCBT Publication Review
Date published: 2015-02-20
Rated 5 out of 5 by from Antibody was used successfully in EMSA usingAntibody was used successfully in EMSA using mouse C19 MEL and human Jurkat, HeLa, K-562, ovarian and breast cells. -SCBT Publication Review
Date published: 2015-01-25
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GATA-1 抗体 (N1) is rated 5.0 out of 5 by 10.
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