Date published: 2025-9-7
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Fra2 Antibody (G-5) - Western Blotting - Image 376172
Fra-2 抗体 (G-5): sc-166102. MCF7 (A), TK-1 (B) 和 U-87 MG (C) 全细胞裂解液中 Fra-2 表达的 Western 印迹分析.
Fra-2 抗体 (G-5): sc-166102. MCF7 (A), TK-1 (B) 和 U-87 MG (C) 全细胞裂解液中 Fra-2 表达的 Western 印迹分析.

Fra2 抗体 (G-5): sc-166102

3.5(4)
寫評論提問

说明书
  • Fra2 抗体 G-5 是小鼠单克隆 IgG2a κ，Fra2抗体, 在5篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Fra-2的氨基酸180-282
  • Fra2 抗体 (G-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Fra-2
  • 抗Fra2抗体（G-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-166102 X, 200 µg/0.1 ml)
  • 关于如何获取Fra2 (G-5): sc-166102的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是Fra2 Antibody (G-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Fra2 Antibody (G-5) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Fra抗体，包括Fra-2

Fra-2抗体（G-5）是一种IgG2a κ小鼠单克隆Fra-2抗体（也称为FRA2抗体、Fos相关抗原2抗体或FOSL2抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Fra-2蛋白。Fra-2抗体（G-5）既有非偶联抗Fra-2抗体形式，也有多种偶联形式的抗Fra-2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Fos相关基因Fra-2最初是从鸡基因组DNA中分子克隆得到的，并被证明是即刻早期基因家族的新成员。鸡Fra-2基因的人类对应物从那时就已被描述。序列比对显示，Fra-2、c-Fos、Fra-1和Fos B之间保守的氨基酸序列包含在五个区域中。区域2是最长且保守性最高的区域，包含亮氨酸拉链结构和基本区域，这表明与Fos、Fra-1和Fos B一样，Fra-2也与c-Jun形成异二聚体，识别特定的DNA序列，如转录因子AP-1的结合位点。在生长刺激的细胞中，Fra-2基因产物与c-Jun形成复合物的发现进一步支持了这一模型。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Fra2 抗体 (G-5) 参考文献:

  1. 由 FRA-2 控制的对低氧损伤的转录反应。  |  Butler, TL. and Pennypacker, KR. 2005. Gene Expr. 12: 61-7. PMID: 15892448
  2. 抑制 NFAT 可增加 Fra-2 的表达，从而促进成骨细胞分化。  |  Zayzafoon, M. 2005. J Musculoskelet Neuronal Interact. 5: 347. PMID: 16340132
  3. 鉴定FRA1和FRA2为参与调节酵母铁调控因子的基因，以应对线粒体铁-硫簇合成的减少。  |  Kumánovics, A., et al. 2008. J Biol Chem. 283: 10276-86. PMID: 18281282
  4. 人类 fos 相关基因的分离及其在单核-巨噬细胞分化过程中的表达。  |  Matsui, M., et al. 1990. Oncogene. 5: 249-55. PMID: 2107490
  5. fos基因家族的另一成员fra-2的分离和特征描述。  |  Nishina, H., et al. 1990. Proc Natl Acad Sci U S A. 87: 3619-23. PMID: 2110368
  6. Fra2 是 Fep1 在铁饥饿时抑制作用的共同调节器。  |  Jacques, JF., et al. 2014. PLoS One. 9: e98959. PMID: 24897379
  7. fos 相关基因的产物 fra-1 与 Jun 共同结合到 AP-1 位点：转录因子 AP-1 由多种蛋白质复合物组成。  |  Cohen, DR., et al. 1989. Genes Dev. 3: 173-84. PMID: 2497053
  8. 一种新型生长因子激活基因的产物 fos B 能与 JUN 蛋白相互作用，增强其 DNA 结合活性。  |  Zerial, M., et al. 1989. EMBO J. 8: 805-13. PMID: 2498083
  9. Fos 相关蛋白 p39 是 jun 原癌基因的产物。  |  Rauscher, FJ., et al. 1988. Science. 240: 1010-6. PMID: 3130660
  10. Fos 蛋白复合物与离体细胞核中的 DNA 相关联，并与 DNA 纤维素结合。  |  Sambucetti, LC. and Curran, T. 1986. Science. 234: 1417-9. PMID: 3491427
  11. ATRA通过抑制系统性硬化症中促纤维化分子Fra2/AP-1来改善纤维化。  |  Pi, Z., et al. 2023. Int Immunopharmacol. 121: 110420. PMID: 37331293
  12. 病毒和细胞的 fos 蛋白与一种 39,000 道尔顿的细胞蛋白复合。  |  Curran, T., et al. 1985. Mol Cell Biol. 5: 167-72. PMID: 3885008

订购信息

产品名称产品编号规格价格数量收藏夹

Fra2 抗体 (G-5)

sc-166102
200 µg/ml
$316.00

Fra2 (G-5): m-IgG Fc BP-HRP 套装

sc-525757
200 µg Ab; 10 µg BP
$354.00

Fra2 (G-5): m-IgG2a BP-HRP 套装

sc-546400
200 µg Ab; 10 µg BP
$354.00

Fra2 抗体 (G-5) X

sc-166102 X
200 µg/0.1 ml
$316.00

Fra2 抗体 (G-5) AC

sc-166102 AC
500 µg/ml, 25% agarose
$416.00

Fra2 抗体 (G-5) HRP

sc-166102 HRP
200 µg/ml
$316.00

Fra2 抗体 (G-5) FITC

sc-166102 FITC
200 µg/ml
$330.00

Fra2 抗体 (G-5) PE

sc-166102 PE
200 µg/ml
$343.00

Fra2 抗体 (G-5) Alexa Fluor® 488

sc-166102 AF488
200 µg/ml
$357.00

Fra2 抗体 (G-5) Alexa Fluor® 546

sc-166102 AF546
200 µg/ml
$357.00

Fra2 抗体 (G-5) Alexa Fluor® 594

sc-166102 AF594
200 µg/ml
$357.00

Fra2 抗体 (G-5) Alexa Fluor® 647

sc-166102 AF647
200 µg/ml
$357.00

Fra2 抗体 (G-5) Alexa Fluor® 680

sc-166102 AF680
200 µg/ml
$357.00

Fra2 抗体 (G-5) Alexa Fluor® 790

sc-166102 AF790
200 µg/ml
$357.00

could you please tell me what is the relationship between sc-166102 and sc-166102 X? if i would like to do chip, should i purchase sc-166102 X? could i do wb,IF and other expriments by sc-166102 X?

Asked by: rsln
Thank you for your question. sc-166102 is recommended for WB, IP, IF, ELISA. If your are performing a Chip Assay we recommend the sc-166102 X since it is 10 x more concentrated.
Answered by: BlakeJ
Date published: 2024-08-16

When should the TransCruz reagent sc-166102 X be used instead of the standard form sc-166102?

Asked by: AbPolly
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from worked ok for human lysates from OCTOCT-embedded human lung tissue was washed with cold HBSS and centrifuged at 10000g, three times. Then, pellet was lysed with SDS buffer (62.5 mM Tris-HCl pH 6.8, 2% SDS and 10% glycerol) and the lysates were used ith standard WB conditions: 20-60μg protein (total extracts in RIPA buffer) per sample was loaded. Membranes were blocked with 5% BSA or non-fat dry milk in PBS-T(1% Tween 20). Antibody was diluted 1/300.Blots were incubated with the appropriate secondary horseradish peroxidase-coupled antibody (GE) and developed using the Luminata Western HRP Substrate (Millipore) and Amersham ECL Hyperfilms (GE Healthcare) or a ChemiDoc XRS+ imaging system with Image Lab image acquisition and analysis software (Bio-Rad)
Date published: 2019-11-28
Rated 1 out of 5 by from Useless antibody for immunohistochemistryI do not know why SCBT discontinued their polyclonal antibodies for several of the transcription factors like Fra-2 and Fos. they worked beautifully in my research. The monoclonal replacements do not work at all. Huge waste of money. They would not even send a sample to test out prior to purchase to determine if it worked.
Date published: 2019-07-29
Rated 5 out of 5 by from Great monoclonal antibodyClean and clear band observed in multiple sample types
Date published: 2017-02-03
Rated 5 out of 5 by from Great Western Blot data of FraGreat Western Blot data of Fra-2 expression in C32 whole cell lysate. -SCBT QC
Date published: 2013-04-15
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Fra2 抗体 (G-5) is rated 3.5 out of 5 by 4.
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