Date published: 2025-9-7
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FKHR/FOXO1 Antibody (C-9) - Western Blotting - Image 349997
FKHR 抗体 (C-9): sc-374427. NIH/3T3 (A), MCF7 (B), TE671 (C) 和 A-431 (D) 全细胞裂解液中 FKHR 表达的 Western 印迹分析.
FKHR 抗体 (C-9): sc-374427. NIH/3T3 (A), MCF7 (B), TE671 (C) 和 A-431 (D) 全细胞裂解液中 FKHR 表达的 Western 印迹分析.

FKHR/FOXO1 抗体 (C-9): sc-374427

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说明书
  • FKHR/FOXO1 抗体 C-9 是小鼠单克隆 IgG1 κ，FKHR/FOXO1抗体, 在46篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的FKHR的C-terminus附近的氨基酸615-653之间
  • FKHR/FOXO1 抗体 (C-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的FKHR; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗FKHR/FOXO1抗体（C-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-374427 X, 200 µg/0.1 ml)
  • 关于如何获取FKHR/FOXO1 (C-9): sc-374427的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 FKHR/FOXO1 抗体 (C-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与FKHR/FOXO1 抗体 (C-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 FKHR 抗体包括 FKHRL1

KHR抗体（C-9）是一种IgG1 κ小鼠单克隆FKHR抗体（也称为FOXO1A抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的FKHR蛋白。FKHR抗体（C-9）既可作为非偶联的抗FKHR抗体形式提供，也可作为多种偶联形式的抗FKHR抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。FKHR（rhabdomyosarcoma中的forkhead）和FKHRL1是forkhead转录因子家族的成员。FKHR蛋白的转录激活受丝氨酸/苏氨酸激酶Akt1的调控，Akt1使FKHRL1磷酸化，导致FKHRL1与14-3-3蛋白结合并滞留在细胞质中。凋亡的诱导或生长因子的撤除刺激FKHR蛋白的去磷酸化和核转位，导致FKHR诱导的基因特异性转录激活。FKHR，也被称为forkhead box protein O1A（FOXO1），是一种在细胞质和细胞核之间穿梭的普遍表达的蛋白质。FKHR基因的遗传突变，包括t(2;13)和t(1;3)易位，常见于肺泡横纹肌肉瘤中。这些易位导致Pax-3或Pax-7的氨基末端（包括配对盒和同源结构域DNA结合域）与含有转录激活域的FKHR的羧基末端融合。Pax-3/FKHR融合蛋白似乎作为一种致癌转录因子发挥作用，可增强正常Pax-3靶基因的激活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FKHR/FOXO1 抗体 (C-9) 参考文献:

  1. Akt 通过磷酸化和抑制叉头转录因子来促进细胞存活。  |  Brunet, A., et al. 1999. Cell. 96: 857-68. PMID: 10102273
  2. Akt 对叉头转录因子 FKHR 的负调控。  |  Tang, ED., et al. 1999. J Biol Chem. 274: 16741-6. PMID: 10358014
  3. 蛋白激酶 B 对转录因子叉头家族成员 FKHR 的磷酸化。  |  Rena, G., et al. 1999. J Biol Chem. 274: 17179-83. PMID: 10358075
  4. 蛋白激酶 B/Akt 介导的磷酸化促进了翼螺旋转录因子 FKHR1 的核排斥。  |  Biggs, WH., et al. 1999. Proc Natl Acad Sci U S A. 96: 7421-6. PMID: 10377430
  5. 多重磷酸化事件对转录因子FKHR、FKHRL1和AFX的影响。  |  Woods, YL. and Rena, G. 2002. Biochem Soc Trans. 30: 391-7. PMID: 12196101
  6. 丝氨酸 256 的磷酸化通过多种机制抑制 FKHR（FOXO1）的转录激活。直接和间接影响核/细胞质穿梭和 DNA 结合。  |  Zhang, X., et al. 2002. J Biol Chem. 277: 45276-84. PMID: 12228231
  7. FOXO1 是调节肿瘤坏死因子诱导成纤维细胞凋亡所需基因表达的总开关。  |  Alikhani, M., et al. 2005. J Biol Chem. 280: 12096-102. PMID: 15632117
  8. psammaplysenes 是 FOXO1a 核输出的特异性抑制剂。  |  Schroeder, FC., et al. 2005. J Nat Prod. 68: 574-6. PMID: 15844952
  9. FOXO1a 是肺泡横纹肌肉瘤的选择性肿瘤抑制因子。  |  Bois, PR., et al. 2005. J Cell Biol. 170: 903-12. PMID: 16157701
  10. 实体瘤肺泡横纹肌肉瘤中叉头域基因与 PAX3 的融合。  |  Galili, N., et al. 1993. Nat Genet. 5: 230-5. PMID: 8275086
  11. 肺泡横纹肌肉瘤中 FKHR 基因及其重排的结构特征。  |  Davis, RJ., et al. 1995. Hum Mol Genet. 4: 2355-62. PMID: 8634710
  12. 组成 FKHR 类基因亚家族的三个人类叉头基因的克隆和特征描述。  |  Anderson, MJ., et al. 1998. Genomics. 47: 187-99. PMID: 9479491

订购信息

产品名称产品编号规格价格数量收藏夹

FKHR/FOXO1 抗体 (C-9)

sc-374427
200 µg/ml
RMB2377.00

FKHR/FOXO1 (C-9): m-IgG Fc BP-HRP 套装

sc-551926
200 µg Ab; 10 µg BP
RMB2662.00

FKHR/FOXO1 抗体 (C-9) X

sc-374427 X
200 µg/0.1 ml
RMB2377.00

FKHR/FOXO1 抗体 (C-9) AC

sc-374427 AC
500 µg/ml, 25% agarose
RMB3129.00

FKHR/FOXO1 抗体 (C-9) HRP

sc-374427 HRP
200 µg/ml
RMB2377.00

FKHR/FOXO1 抗体 (C-9) FITC

sc-374427 FITC
200 µg/ml
RMB2482.00

FKHR/FOXO1 抗体 (C-9) PE

sc-374427 PE
200 µg/ml
RMB2580.00

FKHR/FOXO1 抗体 (C-9) Alexa Fluor® 488

sc-374427 AF488
200 µg/ml
RMB2685.00

FKHR/FOXO1 抗体 (C-9) Alexa Fluor® 546

sc-374427 AF546
200 µg/ml
RMB2685.00

FKHR/FOXO1 抗体 (C-9) Alexa Fluor® 594

sc-374427 AF594
200 µg/ml
RMB2685.00

FKHR/FOXO1 抗体 (C-9) Alexa Fluor® 647

sc-374427 AF647
200 µg/ml
RMB2685.00

FKHR/FOXO1 抗体 (C-9) Alexa Fluor® 680

sc-374427 AF680
200 µg/ml
RMB2685.00

FKHR/FOXO1 抗体 (C-9) Alexa Fluor® 790

sc-374427 AF790
200 µg/ml
RMB2685.00

FKHR/FOXO1 (C-9) 中和勝肽

sc-374427 P
100 µg/0.5 ml
RMB511.00

What is the recommended concentration to use with flow cytometry?

Asked by: Sheetal
Thank you for your question. 1 µg normal immunoglobulins per 1 million cells. https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2023-02-15

Is this antibody tested for CHIP application? If yes, please let me know where the results of Chip published. thanks

Asked by: GBhardwaj
Thank you for your question. This antibody has not yet been tested for CHiP so that is not a recommended application at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service 1
Date published: 2021-02-04

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-10
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Rated 4 out of 5 by from provato in IF su sezione paraffinata, funziona!Il sample provato in immunofuorescenza su sezioni di tessuto di mucosa del colon umana ha funzionato
Date published: 2024-02-15
Rated 1 out of 5 by from PoorPoor product services, didn't describe clearly on the product....
Date published: 2022-04-13
Rated 5 out of 5 by from 抗体很好用！该抗体很稳定，western blot效果不错，条带清晰！值得购买！下次有需要会再购买，希望越做越好！
Date published: 2017-12-07
Rated 2 out of 5 by from No bandsI obtained this from Santa Cruz Biotechnology Shanghai a month ago.Western bolt analysis of extracts from various cell lines using it and there is no band on membrane.
Date published: 2017-10-25
Rated 4 out of 5 by from GREAT!Great! This antibody worked great for western blot.
Date published: 2017-10-13
Rated 3 out of 5 by from 可以检测到条带，位置不对我检测的是小鼠肝脏组织，配置比列是1：500，可以检测到条带，位置不对,,,,,,,,,,,,,,,,,
Date published: 2017-09-06
Rated 4 out of 5 by from Good for WBWb used rat protein with a dilution of 1:500,the band is special but not so clear,I will tried with a dilution of 1:200 to try again
Date published: 2017-05-04
Rated 1 out of 5 by from No detection in mouse liverWe tested a trial aliquot of this antibody on 40 ug mouse liver samples, according to the recommended protocols and saw no detection.
Date published: 2017-04-12
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FKHR/FOXO1 抗体 (C-9) is rated 4.0 out of 5 by 16.
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