Date published: 2025-9-7
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FLG/Filaggrin Antibody (AKH1) - Immunohistochemistry - Image 361443
Filaggrin 抗体 (AKH1): sc-66192. 福尔马林固定, 石蜡包埋的人外阴/肛门皮肤组织的免疫过氧化物酶染色显示角质层染色.
FLG/Filaggrin Antibody (AKH1) - Immunohistochemistry - Image 361443
Filaggrin 抗体 (AKH1): sc-66192. 福尔马林固定, 石蜡包埋的人外阴/肛门皮肤组织的免疫过氧化物酶染色显示角质层染色.
FLG/Filaggrin Antibody (AKH1) - Immunohistochemistry - Image 361444
Filaggrin 抗体 (AKH1): sc-66192. 福尔马林固定, 石蜡包埋的人皮肤组织的免疫过氧化物酶染色显示角质层染色.
Filaggrin 抗体 (AKH1): sc-66192. 福尔马林固定, 石蜡包埋的人外阴/肛门皮肤组织的免疫过氧化物酶染色显示角质层染色.

FLG/Filaggrin 抗体 (AKH1): sc-66192

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说明书
  • FLG/Filaggrin 抗体 AKH1 是小鼠单克隆 IgG1 κ，FLG/Filaggrin抗体, 在98篇文献中引用，规格为200 µg/ml
  • 针对human来源的纯化包皮Filaggrin
  • FLG/Filaggrin 抗体 (AKH1) 推荐用于WB, IF 和 IHC(P)，检测human 来源的 Filaggrin and Profilaggrin
  • 抗FLG/Filaggrin抗体（AKH1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FLG/Filaggrin (AKH1): sc-66192的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 FLG/Filaggrin 抗体 (AKH1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FLG/Filaggrin 抗体 (AKH1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Filaggrin 抗体包括 Filaggrin 2

Filaggrin 抗体（AKH1）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过西部印迹（WB）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测人源 Filaggrin 蛋白。抗 Filaggrin 抗体 (AKH1) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物，可满足不同的实验需求。Filaggrin 是一种关键的结构蛋白，通过促进角蛋白中间丝的聚集，在皮肤的屏障功能中发挥着至关重要的作用，而角蛋白中间丝的聚集对于维持表皮的完整性和弹性至关重要。Filaggrin 主要位于表皮的角质透明蛋白颗粒中，合成为一种称为 profilaggrin 的大型前体。在角质形成细胞的末期分化过程中，profilaggrin 会被蛋白水解为活性丝胶蛋白，这有助于形成坚固的粟粒状包膜，抵御环境压力并防止水分流失。丝胶蛋白的正常功能对皮肤健康至关重要，因为丝胶蛋白基因突变与特应性皮炎和寻常鱼鳞病等多种皮肤疾病有关，这凸显了丝胶蛋白在皮肤屏障功能和整体皮肤健康中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FLG/Filaggrin 抗体 (AKH1) 参考文献:

  1. 丝胶蛋白的诱导表达增加了角质细胞对细胞凋亡的敏感性。  |  Kuechle, MK., et al. 2000. Cell Death Differ. 7: 566-73. PMID: 10822280
  2. 丝聚蛋白突变与皮肤和过敏性疾病有关。  |  Irvine, AD., et al. 2011. N Engl J Med. 365: 1315-27. PMID: 21991953
  3. 人类 profilaggrin 基因的组织, 结构和多态性。  |  Gan, SQ., et al. 1990. Biochemistry. 29: 9432-40. PMID: 2248957
  4. 丝聚蛋白和皮肤屏障功能。  |  Kezic, S. and Jakasa, I. 2016. Curr Probl Dermatol. 49: 1-7. PMID: 26844893
  5. 编码人类丝胶蛋白的 cDNA 克隆的特征及该基因在染色体 1q21 区域的定域。  |  McKinley-Grant, LJ., et al. 1989. Proc Natl Acad Sci U S A. 86: 4848-52. PMID: 2740331
  6. 丝聚蛋白在特应性皮炎和过敏性疾病中的作用。  |  Drislane, C. and Irvine, AD. 2020. Ann Allergy Asthma Immunol. 124: 36-43. PMID: 31622670
  7. 重新审视丝聚蛋白在特应性皮炎中的作用。  |  Moosbrugger-Martinz, V., et al. 2022. Int J Mol Sci. 23: PMID: 35628125
  8. 二氟拉斯特抑制PDE4，通过人类角质形成细胞中的角蛋白细胞富脯氨酸蛋白调节丝聚蛋白和洛里蛋白的表达。  |  Tsuji, G., et al. 2023. J Dermatol Sci. 110: 61-68. PMID: 37156706
  9. 致病性丝聚蛋白变异对特应性皮炎中杜匹单抗治疗的影响。  |  Clabbers, J., et al. 2024. J Allergy Clin Immunol. 153: 1155-1161.e4. PMID: 38272373
  10. 丝聚蛋白突变状态和母体益生菌补充预防特应性皮炎。  |  Zakiudin, DP., et al. 2024. Acta Derm Venereol. 104: adv24360. PMID: 38655655
  11. Filaggrin 链接段肽和胱抑素 alpha 是表皮粟粒状包膜复合物的组成部分。  |  Takahashi, M., et al. 1996. Arch Biochem Biophys. 329: 123-6. PMID: 8619628
  12. 活动性银屑病斑块边缘, 银屑病患者未受累皮肤和正常健康皮肤中循环表皮细胞的招募以及丝胶蛋白, 卷曲蛋白和韧带蛋白的表达。  |  Gerritsen, MJ., et al. 1997. J Dermatol Sci. 14: 179-88. PMID: 9138475

订购信息

产品名称产品编号规格价格数量收藏夹

FLG/Filaggrin 抗体 (AKH1)

sc-66192
200 µg/ml
RMB2377.00

FLG/Filaggrin (AKH1): m-IgG Fc BP-HRP 套装

sc-526977
200 µg Ab; 10 µg BP
RMB2662.00

FLG/Filaggrin (AKH1): m-IgG1 BP-HRP 套装

sc-532350
200 µg Ab; 20 µg BP
RMB2662.00

FLG/Filaggrin 抗体 (AKH1) AC

sc-66192 AC
500 µg/ml, 25% agarose
RMB3129.00

FLG/Filaggrin 抗体 (AKH1) HRP

sc-66192 HRP
200 µg/ml
RMB2377.00

FLG/Filaggrin 抗体 (AKH1) FITC

sc-66192 FITC
200 µg/ml
RMB2482.00

FLG/Filaggrin 抗体 (AKH1) PE

sc-66192 PE
200 µg/ml
RMB2580.00

FLG/Filaggrin 抗体 (AKH1) Alexa Fluor® 488

sc-66192 AF488
200 µg/ml
RMB2685.00

FLG/Filaggrin 抗体 (AKH1) Alexa Fluor® 546

sc-66192 AF546
200 µg/ml
RMB2685.00

FLG/Filaggrin 抗体 (AKH1) Alexa Fluor® 594

sc-66192 AF594
200 µg/ml
RMB2685.00

FLG/Filaggrin 抗体 (AKH1) Alexa Fluor® 647

sc-66192 AF647
200 µg/ml
RMB2685.00

FLG/Filaggrin 抗体 (AKH1) Alexa Fluor® 680

sc-66192 AF680
200 µg/ml
RMB2685.00

FLG/Filaggrin 抗体 (AKH1) Alexa Fluor® 790

sc-66192 AF790
200 µg/ml
RMB2685.00

Hello, could you please give us some technical details (antigen retrieval, incubation time...) to properly use this antibody in IHC (automated system)? Thank you in advance, Giorgia

Asked by: IFOGio
Thank you for your question. FLG/Filaggrin Antibody (AKH1): sc-66192 has only been tested in manual IHC/IHC(P) applications. For antigen retrieval we recommend Heat treatment: Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides. You can find our full recommended protocol under this link: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Support Europe
Date published: 2025-04-03

We are interested in using sc-66192 AF488 for IHC(p) skin sections however couldnt find an appropriate protocol for doing so. Request you to provide the protocol of same if available.

Asked by: Sandeep
Thank you for contacting Santa Cruz Biotechnology. We do not have a protocol for skin sections. Here is our IHC protocol: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: BlakeJ
Date published: 2024-07-09

Hello, How did you prove the specificity of this Ab? Do you have images with this Ab preincubated with the excess of the immunizing peptide that show loss of staining? Thanks

Asked by: mrochman
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-06-09

Dear SantaCruz Team, might it be possible to order this particular antibody in a carrier-free version, i.e. without gelatin or sodium azide? We would need it to test prior to imaging mass cytometry (IMC) application.

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-01-20

I'm going to have a human skin IHC staining (in paraffin embedded sections). Therefore, I want to check if you know what's the retrieval protocol to make the better staining.

Asked by: Louis0007
Thanks for your question. Certain antigenic determinants are masked by formalin fixation and paraffin embedding and may be exposed by one of several methods: Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides. Pepsin: Incubate sections for 10–20 minutes in 0.1% Pepsin (sc-476554) in 0.01 N HCl at room temperature. Wash slides several times in deionized H2O. Aspirate excess liquid from slides. Saponin: Incubate sections for 30 minutes in 0.05% Saponin (sc-280079) in deionized H2O at room temperature. Wash at least three times in PBS. Aspirate excess liquid from slides. Here is the link to IHC for your reference:https://www.scbt.com/zh/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 9
Date published: 2021-09-07

Please do you have image showing Western Blot for Cat No. Filaggrin Antibody (AKH1): sc-66192 showing specificity to mouse?

Asked by: Tawfik
We do not have published pictures of this antibody used in a Western Blot on mouse. Please contact Technical Support for more information.
Answered by: Tech Sales
Date published: 2021-03-10

Do you have any more precise information about location of AKH1 epitope on FLG?

Asked by: Dusko
Thank you for your question. No epitope mapping has been performed for this antibody, so the exact epitope it binds to is not known.
Answered by: Technical Support Europe
Date published: 2020-10-28

I am looking for Filaggrin Antibody to use it in my Western Blot experiment. I am looking for anti-Human, anti-Pig and anti-mouse specificity.

Asked by: TMISSA
Thank you for your question. Filaggrin Antibody (AKH1): sc-66192 is recommended for detection of human Filaggrin, and we have not yet tested the antibody for reactivity with additional species. We also offer a different antibody, Filaggrin Antibody (AE21): sc-80609, but this is also only currently recommended for detection of human Filaggrin. For a limited time we are offering a free 10µg sample of our monoclonal antibody, Filaggrin (AKH1): sc-66192. Please contact our Technical Service Department to request a sample. Please call us toll-free at (800) 457-3801 option 2, e-mail [email protected], or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-05-23
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Rated 5 out of 5 by from Works well in organotypic cultureWorks well as a stain in organotypic culture for immunofluorescence. Specific staining.
Date published: 2021-07-16
Rated 4 out of 5 by from Good for western blotFor western blot, use at 1:200 dilution in 5% milk TBST, overnight incubation with rocking at 4 degrees C. Example of published result: PMID 29134204.
Date published: 2020-01-09
Rated 5 out of 5 by from Worked with human skin staining with IHCWe received a trail size of filaggrin antibody to staining human skin and it worked well.
Date published: 2018-01-03
Rated 5 out of 5 by from Cross reacts with mouse filaggrinIn Western Blot analysis, a 45 kDa band was observed from mouse epidermis
Date published: 2017-12-15
Rated 5 out of 5 by from ExcellentThis antibody works extremely well in Paraformaldehyde fixed, cryosectioned human epidermis sections.
Date published: 2017-08-31
Rated 5 out of 5 by from strong band seenThis monoclonal antibody gives a nice strong robust and clean band for WB analysis in Hela whole cell lysate.
Date published: 2017-05-28
Rated 5 out of 5 by from works wellPositive in human skin tissue, works well for IF applications.
Date published: 2017-02-12
Rated 5 out of 5 by from Excellent IHC antibodyStrong and specific staining observed by IHC in paraffin embedded sections
Date published: 2017-01-13
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FLG/Filaggrin 抗体 (AKH1) is rated 4.7 out of 5 by 12.
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