Date published: 2025-9-8

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Fibrillarin 抗体 (G-4): sc-166021

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说明书
  • Fibrillarin抗体(G-4)是小鼠单克隆IgG2b κ, 在5篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Fibrillarin的氨基酸61-200
  • 抗-Fibrillarin 抗体 (G-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Fibrillarin
  • 抗Fibrillarin抗体(G-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Fibrillarin (G-4): sc-166021免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 Fibrillarin 抗体 (G-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Fibrillarin 抗体 (G-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

纤维蛋白抗体(G-4)是一种IgG2b κ小鼠单克隆纤维蛋白抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的纤维蛋白。纤维蛋白抗体(G-4)既可作为非偶联抗纤维蛋白抗体形式,也可作为多种偶联形式的抗纤维蛋白抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。纤维蛋白是一种广泛存在的基本非组蛋白,专门定位于核仁的纤维状区域,包括致密的纤维状中心和纤维状中心区域。纤维蛋白也在HeLa细胞、3T3细胞和人外周血淋巴细胞中表达。在有丝分裂中期和后期,纤维蛋白位于假定的染色体核仁区域(NORs)。在末期,纤维蛋白是新形成核仁部位的早期标记物。纤维蛋白的结构包括一个RNA结合域和一个RNP共识序列,这与纤维蛋白与U3小核仁RNA的结合是一致的。纤维蛋白参与核仁中rRNA转录本的处理。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Fibrillarin 抗体 (G-4) 参考文献:

  1. 系统性硬化症中的抗纤连蛋白抗体。  |  Tormey, VJ., et al. 2001. Rheumatology (Oxford). 40: 1157-62. PMID: 11600746
  2. 放线菌素 D 改变了核仁蛋白纤维蛋白在 HEp-2 细胞中的亚细胞分布。  |  Chen, M. and Jiang, P. 2004. Acta Pharmacol Sin. 25: 902-6. PMID: 15210063
  3. 弓形虫纤维素-Nop5异源二聚体独立于C/D向导RNP颗粒对RNA进行2'-O-甲基化。  |  Tomkuvienė, M., et al. 2017. RNA. 23: 1329-1337. PMID: 28576826
  4. 核小体中 pol I 和纤维素含量的波动。  |  Hornáček, M., et al. 2017. Nucleus. 8: 421-432. PMID: 28622108
  5. 纤丝蛋白核糖核酸酶的活性取决于 GAR 结构域并受磷脂调节  |  Guillen-Chable, F., et al. 2020. Cells. 9: PMID: 32384686
  6. 肝细胞癌中纤维蛋白原表达的增加与肿瘤进展和预后不良有关。  |  Zhang, J., et al. 2021. Oncol Lett. 21: 92. PMID: 33376525
  7. 真核生物 Fibrillarin 与 Nop56 氨基终止子相互作用的高分辨率结构。  |  Höfler, S., et al. 2021. RNA. 27: 496-512. PMID: 33483369
  8. 纤维素 LC 结构域的相变调节纤维素的定位和蛋白质相互作用。  |  Kim, E. and Kwon, I. 2021. Biochem J. 478: 799-810. PMID: 33522570
  9. 纤丝蛋白 RNA 甲基化酶是一种与副隐孢子虫钙调蛋白样蛋白(CpCML)相互作用的蛋白质。  |  Sun, T., et al. 2022. Microb Pathog. 170: 105679. PMID: 35843442
  10. 贾第虫纤维素:序列和结构的生物信息学探索。  |  Lagunas-Rangel, FA. 2024. J Appl Genet.. PMID: 39527366

订购信息

产品名称产品编号规格价格数量收藏夹

Fibrillarin 抗体 (G-4)

sc-166021
200 µg/ml
RMB2377.00

Fibrillarin (G-4): m-IgGκ BP-HRP 套装

sc-521485
200 µg Ab, 40 µg BP
RMB2662.00

Fibrillarin (G-4): m-IgG2b BP-HRP 套装

sc-548911
200 µg Ab; 10 µg BP
RMB2662.00

Fibrillarin 抗体 (G-4) AC

sc-166021 AC
500 µg/ml, 25% agarose
RMB3129.00

Fibrillarin 抗体 (G-4) HRP

sc-166021 HRP
200 µg/ml
RMB2377.00

Fibrillarin 抗体 (G-4) FITC

sc-166021 FITC
200 µg/ml
RMB2482.00

Fibrillarin 抗体 (G-4) PE

sc-166021 PE
200 µg/ml
RMB2580.00

Fibrillarin 抗体 (G-4) Alexa Fluor® 488

sc-166021 AF488
200 µg/ml
RMB2685.00

Fibrillarin 抗体 (G-4) Alexa Fluor® 546

sc-166021 AF546
200 µg/ml
RMB2685.00

Fibrillarin 抗体 (G-4) Alexa Fluor® 594

sc-166021 AF594
200 µg/ml
RMB2685.00

Fibrillarin 抗体 (G-4) Alexa Fluor® 647

sc-166021 AF647
200 µg/ml
RMB2685.00

Fibrillarin 抗体 (G-4) Alexa Fluor® 680

sc-166021 AF680
200 µg/ml
RMB2685.00

Fibrillarin 抗体 (G-4) Alexa Fluor® 790

sc-166021 AF790
200 µg/ml
RMB2685.00

I am using Fibrillarin (G-4): sc-166021 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_30, h_9CST
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Rated 5 out of 5 by from Ottimo anticorpoQuesto anticorpo funziona molto bene in immunoistochimica sia applicata alla microscopia a fluorescenza che alla microscopia elettronica a trasmissione. Ottima resa anche in Western Blot.
Date published: 2020-11-13
Rated 5 out of 5 by from Great antibodyFibrillarin Antibody (G-4) produced clear bands and specific staining with little to no background. Great for WB, IHCP and IF
Date published: 2017-01-25
Rated 5 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of Fibrillarin expression in Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by from Works great for mouse Fibrillarin tranfectedWorks great for mouse Fibrillarin tranfected lysates. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear stainingProduced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human breast tissue. -SCBT QC
Date published: 2015-02-24
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Fibrillarin 抗体 (G-4) is rated 5.0 out of 5 by 5.
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