Date published: 2025-9-8

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Fibrillarin 抗体 (B-1): sc-166001

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说明书
  • Fibrillarin抗体(B-1)是小鼠单克隆IgG1 κ, 在23篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Fibrillarin的氨基酸61-200
  • 抗-Fibrillarin 抗体 (B-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Fibrillarin
  • 抗Fibrillarin抗体(B-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Fibrillarin (B-1): sc-166001免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Fibrillarin 抗体 (B-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Fibrillarin 抗体 (B-1) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

纤维蛋白抗体(B-1)是一种IgG1 κ小鼠单克隆纤维蛋白抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的纤维蛋白。纤维蛋白抗体(B-1)既可作为非偶联抗纤维蛋白抗体形式,也可作为多种偶联形式的抗纤维蛋白抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。纤维蛋白是一种广泛存在的基本非组蛋白,专门定位于核仁的纤维状区域,包括致密的纤维状中心和纤维状中心区域。纤维蛋白也在HeLa细胞、3T3细胞和人外周血淋巴细胞中表达。在有丝分裂中期和后期,纤维蛋白位于假定的染色体核仁区域(NORs)。在末期,纤维蛋白是新形成核仁部位的早期标记物。纤维蛋白的结构包括一个RNA结合域和一个RNP共识序列,这与纤维蛋白与U3小核仁RNA的结合是一致的。纤维蛋白参与核仁中rRNA转录本的处理。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Fibrillarin 抗体 (B-1) 参考文献:

  1. 核仁的结构和功能  |  Scheer, U. and Hock, R. 1999. Curr Opin Cell Biol. 11: 385-90. PMID: 10395554
  2. 核球:一个拥有意想不到能力的老工厂。  |  Olson, MO., et al. 2000. Trends Cell Biol. 10: 189-96. PMID: 10754561
  3. 哺乳动物细胞核中蛋白质的高流动性。  |  Phair, RD. and Misteli, T. 2000. Nature. 404: 604-9. PMID: 10766243
  4. 纤毛素对小鼠的早期发育至关重要,是内含子编码的小核仁核糖核酸积累所必需的。  |  Newton, K., et al. 2003. Mol Cell Biol. 23: 8519-27. PMID: 14612397
  5. 超嗜热古生物 Pyrococcus furiosus 纤维素的结构测定。  |  Deng, L., et al. 2004. Biochem Biophys Res Commun. 315: 726-32. PMID: 14975761
  6. 人纤维蛋白的 cDNA 克隆和测序,人纤维蛋白是一种被自身免疫抗血清识别的保守核小体蛋白。  |  Aris, JP. and Blobel, G. 1991. Proc Natl Acad Sci U S A. 88: 931-5. PMID: 1846968
  7. 人类核仁蛋白纤维蛋白的进化保护及其在酵母中的功能表达  |  Jansen, RP., et al. 1991. J Cell Biol. 113: 715-29. PMID: 2026646
  8. 纤丝蛋白:通过自身免疫血清发现的一种新的核仁蛋白。  |  Ochs, RL., et al. 1985. Biol Cell. 54: 123-33. PMID: 2933102
  9. 核苷酸纤维素是细菌病原体抗性的进化保守调节因子。  |  Tiku, V., et al. 2018. Nat Commun. 9: 3607. PMID: 30190478
  10. Fibrillarin-GFP 有助于鉴定具有减数分裂能力的卵母细胞  |  Wang, T. and Na, J. 2021. Front Cell Dev Biol. 9: 648331. PMID: 33937243
  11. 纤连蛋白能促进重组酶 RAD51 招募到 DNA 损伤位点,从而促进同源重组修复。  |  Mu, Y., et al. 2023. J Zhejiang Univ Sci B. 24: 1165-1173. PMID: 38057273
  12. 纤丝蛋白同源物在扁平动物的稳态和再生过程中调控不同细胞系的翻译。  |  Chen, J., et al. 2024. EMBO J. 43: 6591-6625. PMID: 39567829

订购信息

产品名称产品编号规格价格数量收藏夹

Fibrillarin 抗体 (B-1)

sc-166001
200 µg/ml
RMB2377.00

Fibrillarin (B-1): m-IgG Fc BP-HRP 套装

sc-528891
200 µg Ab; 10 µg BP
RMB2662.00

Fibrillarin (B-1): m-IgGκ BP-HRP 套装

sc-521481
200 µg Ab, 40 µg BP
RMB2662.00

Fibrillarin (B-1): m-IgG1 BP-HRP 套装

sc-543196
200 µg Ab; 20 µg BP
RMB2662.00

Fibrillarin 抗体 (B-1) AC

sc-166001 AC
500 µg/ml, 25% agarose
RMB3129.00

Fibrillarin 抗体 (B-1) HRP

sc-166001 HRP
200 µg/ml
RMB2377.00

Fibrillarin 抗体 (B-1) FITC

sc-166001 FITC
200 µg/ml
RMB2482.00

Fibrillarin 抗体 (B-1) PE

sc-166001 PE
200 µg/ml
RMB2580.00

Fibrillarin 抗体 (B-1) Alexa Fluor® 488

sc-166001 AF488
200 µg/ml
RMB2685.00

Fibrillarin 抗体 (B-1) Alexa Fluor® 546

sc-166001 AF546
200 µg/ml
RMB2685.00

Fibrillarin 抗体 (B-1) Alexa Fluor® 594

sc-166001 AF594
200 µg/ml
RMB2685.00

Fibrillarin 抗体 (B-1) Alexa Fluor® 647

sc-166001 AF647
200 µg/ml
RMB2685.00

Fibrillarin 抗体 (B-1) Alexa Fluor® 680

sc-166001 AF680
200 µg/ml
RMB2685.00

Fibrillarin 抗体 (B-1) Alexa Fluor® 790

sc-166001 AF790
200 µg/ml
RMB2685.00

What is the recommended fixation method for immunofluorescence staining with Fibrillarin (B-1): sc-166001 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibodyIt works nicely on WB with whole cell extracts from Jurkat and k562 cells.
Date published: 2024-05-01
Rated 4 out of 5 by from IP效果不太好,WB效果不错FBL抗体(sc-374022) IP 实验之后的WB检测 细胞系:PA1 效果较好 但是用该抗体做IP效果不好
Date published: 2017-11-15
Rated 5 out of 5 by from Great specific western blot bandWe used this antibody for western blot and got a great specific band for the correct molecular weight
Date published: 2017-01-17
Rated 5 out of 5 by from Very good in immunofluorescenceI received this antibody as a trial size and it was perfect in IF of colon cancer cells.
Date published: 2016-12-22
Rated 5 out of 5 by from Western blot: cleanWestern blot: clean, clear bands in HeLa and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-06-15
Rated 5 out of 5 by from Robust band present in western blot usingRobust band present in western blot using Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-05-23
Rated 5 out of 5 by from Exceptional immunofluorescence nucleolarExceptional immunofluorescence nucleolar staining in formalin-fixed Hep G2 cells. -SCBT QC
Date published: 2015-05-10
Rated 5 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of Fibrillarin expression in non-transfected and mouse Fibrillarin transfected 293T whole cell lysates and MOLT-4 nuclear extract. -SCBT QC
Date published: 2015-04-02
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Fibrillarin 抗体 (B-1) is rated 4.8 out of 5 by 8.
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