Date published: 2025-9-7
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FGFR-3 Antibody (B-9) - Immunohistochemistry - Image 383000
FGFR-3 抗体 (B-9): sc-13121. 经福尔马林固定, 石蜡包埋的人类睾丸组织的免疫过氧化物酶染色显示, 曲细精管中的亚群细胞出现膜和细胞质染色, 以及 Leydig 细胞出现细胞质染色.
FGFR-3 抗体 (B-9): sc-13121. 经福尔马林固定, 石蜡包埋的人类睾丸组织的免疫过氧化物酶染色显示, 曲细精管中的亚群细胞出现膜和细胞质染色, 以及 Leydig 细胞出现细胞质染色.

FGFR-3 抗体 (B-9): sc-13121

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说明书
  • FGFR-3抗体(B-9)是小鼠单克隆IgG2a κ, 在133篇文献中引用，规格为200 µg/ml
  • 免疫human物种的FGFR-3的氨基酸25-124
  • FGFR-3 抗体 (B-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 FGFR-3
  • FGFR-3抗体（B-9）可与Alexa Fluor® 546或Alexa Fluor® 594结合，用于WB（RGB）、IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FGFR-3 (B-9): sc-13121的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 FGFR-3 抗体 (B-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FGFR-3 抗体 (B-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 FGFR 抗体 包括 FGFR-4

FGFR-3抗体（B-9）是一种IgG2aκ小鼠单克隆FGFR-3抗体（也称为FGFR-3抗体或FGFR3抗体），可通过WB、IP、IF、IHC（P）和ELISA检测人源的FGFR-3。FGFR-3抗体（B-9）既可以是偶联的，也可以是非偶联的抗FGFR-3抗体形式。酸性和碱性成纤维细胞生长因子（FGFs）是一类多功能多肽生长因子的成员，可刺激间充质、上皮和神经外胚层细胞的增殖。与其他生长因子一样，FGFs通过结合和激活特定的细胞表面受体来起作用。这些受体通常包含一个含有三个免疫球蛋白样域的细胞外配体结合区域、一个跨膜区域和一个细胞质酪氨酸激酶区域。编码人类FGFR-3的基因定位于染色体4p16，可通过可选剪接产生三种异构体，这些异构体在大脑、肾脏和睾丸中表达。FGFR-3的缺陷与多种疾病有关，包括克劳森综合征、软骨发育不全症、死亡症候群、阿德莱德颅骨合并症和低胸症。FGFR-3的突变也是某些膀胱和宫颈癌的原因。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FGFR-3 抗体 (B-9) 参考文献:

  1. 人类膀胱癌和宫颈癌中 FGFR3 的频繁激活突变。  |  Cappellen, D., et al. 1999. Nat Genet. 23: 18-20. PMID: 10471491
  2. 单冠突触症和成纤维细胞生长因子受体 3 基因突变患者的临床特征：初步报告。  |  Cassileth, LB., et al. 2001. Plast Reconstr Surg. 108: 1849-54. PMID: 11743367
  3. 小鼠 BEK 成纤维细胞生长因子（FGF）受体的特征：FGF 家族三个成员的激活和肝素的要求。  |  Mansukhani, A., et al. 1992. Proc Natl Acad Sci U S A. 89: 3305-9. PMID: 1373495
  4. 克隆和表达与酸性和碱性成纤维细胞生长因子交叉反应的两种不同的高亲和力受体。  |  Dionne, CA., et al. 1990. EMBO J. 9: 2685-92. PMID: 1697263
  5. 成纤维细胞生长因子受体-3（FGFR-3）通过TCF4/β-catenin依赖性和非依赖性信号通路调节肠上皮细胞中paneth细胞系特异性基因的表达。  |  Brodrick, B., et al. 2011. J Biol Chem. 286: 18515-25. PMID: 21388956
  6. 碱性成纤维细胞生长因子细胞生物学的最新发展。  |  Rifkin, DB. and Moscatelli, D. 1989. J Cell Biol. 109: 1-6. PMID: 2545723
  7. 成纤维细胞生长因子（FGF-2）及其受体FGFR-2和FGFR-3可能是潜在恶性口腔病变转化为口腔鳞状细胞癌的潜在生物标志物。  |  Nayak, S., et al. 2015. PLoS One. 10: e0138801. PMID: 26465941
  8. 豚鼠脑中的 25 000 肝素结合蛋白是一种高分子量的碱性成纤维细胞生长因子。  |  Moscatelli, D., et al. 1987. Proc Natl Acad Sci U S A. 84: 5778-82. PMID: 3475702
  9. 成纤维细胞生长因子受体 3 (FGFR3) 跨膜突变在克鲁宗综合征伴黑棘皮病中的应用。  |  Meyers, GA., et al. 1995. Nat Genet. 11: 462-4. PMID: 7493034
  10. 在 FGFR-3 基因的替代 IIIb 和 IIIc 外显子之间做出选择并不具有严格的组织特异性。  |  Scotet, E. and Houssaint, E. 1995. Biochim Biophys Acta. 1264: 238-42. PMID: 7495869
  11. 一名患有软骨发育不全症的新生儿的成纤维细胞生长因子受体-3跨膜结构域中的甘氨酸-375-半胱氨酸置换。  |  Superti-Furga, A., et al. 1995. Eur J Pediatr. 154: 215-9. PMID: 7758520
  12. 由成纤维细胞生长因子受体 3 的不同突变引起的甲状腺发育不良（I 型和 II 型）。  |  Tavormina, PL., et al. 1995. Nat Genet. 9: 321-8. PMID: 7773297

订购信息

产品名称产品编号规格价格数量收藏夹

FGFR-3 抗体 (B-9)

sc-13121
200 µg/ml
RMB2377.00

FGFR-3 (B-9): m-IgG Fc BP-HRP 套装

sc-525465
200 µg Ab; 10 µg BP
RMB2662.00

FGFR-3 (B-9): m-IgG2a BP-HRP 套装

sc-546271
200 µg Ab; 10 µg BP
RMB2662.00

FGFR-3 抗体 (B-9) Alexa Fluor® 546

sc-13121 AF546
200 µg/ml
RMB2685.00

FGFR-3 抗体 (B-9) Alexa Fluor® 594

sc-13121 AF594
200 µg/ml
RMB2685.00

FGFR-3 抗体 (B-9) Alexa Fluor® 680

sc-13121 AF680
200 µg/ml
RMB2685.00

FGFR-3 抗体 (B-9) Alexa Fluor® 790

sc-13121 AF790
200 µg/ml
RMB2685.00

what is the total volume of this vial? 1 ml or 100 microlitres?

Asked by: Tarek
This antibody comes in 1ml containing 200ug of antibody.
Answered by: Dallas Tech Service
Date published: 2021-06-18

Hi, what incubation time do you suggest?

Asked by: Assunta
Thank you for your question. The incubation time depends on the antibody and the samples you are working on, as well as what application you are using it for. In general for WB, we recommend a starting dilution of 1:100, and for IF/IHC, we recommend a starting dilution of 1:50. You can find all this information and more in our recommended protocols: https://www.scbt.com/resources/protocols
Answered by: Tech Service
Date published: 2019-09-27

Hi, can we run this antibody in urinary bladder tumour? 

Asked by: Muhammad Asif
Thank you for your question. We have published data on our website using this antibody in formalin fixed, paraffin-embedded human urinary bladder tissue. We have not tested the antibody specifically in urinary bladder tumour.
Answered by: Technical Support
Date published: 2018-12-14

I need to use a FGFR-3 antibody to detect the protein in samples of non-mammalian origin. Do you know if this monoclonal antibody will work?

Asked by: DefinitelyNotMatt
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 9
Date published: 2016-12-15

What is the recommended detection reagent to be used with this mouse monoclonal primary, if you are no longer selling traditional secondary antibodies?

Asked by: DefinitelyNotMatt
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 9
Date published: 2016-12-15
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Rated 5 out of 5 by from Antibody staining human testis by IHC-PAntibody at 1/100 staining germ cells of the adult human testis by IHC-P.  The tissue was blocked with both BSA and serum and fixed prior to incubation with the antibody for 8 hours at 4°. A biotinylated secondary antibody was used . An Alexa Fluor 594 conjugated goat anti-mouse antibody was used as the secondary.
Date published: 2019-03-25
Rated 2 out of 5 by from Used in WesternsUsed with 1:500 dilution in western blots and was able to obtain very faint bands. However bands were specific with low background. Nevertheless, sc-390423 (E-7) is superior.
Date published: 2018-03-28
Rated 3 out of 5 by from Detects ectopic FGFR3 via flow cytometryAntibody successfully detected ectopically expressed FGFR3 via flow cytometry
Date published: 2017-11-28
Rated 1 out of 5 by from Did not work on mouse brain tissueImmunofluorescence (1:200) did not work on frozen section from 4% PFA fixed embryonic mouse brain tissue. 3-5 min retrieval with citric buffer (PH6.0).
Date published: 2017-10-11
Rated 5 out of 5 by from WB antibodyVery good antibody In the experiment, 1:1000 used always, good band for WB
Date published: 2017-04-27
Rated 5 out of 5 by from Good for Western blotDetects strong bands at expected glycosylated weight
Date published: 2016-12-19
Rated 3 out of 5 by from Good specificity but low sensitivity in IHCGood specificity but low sensitivity in IHC for ASTMA-23, Blad2, Blad4 & Cell Line Microarray. Weak even at 1:50
Date published: 2016-06-13
Rated 5 out of 5 by from We use this often in WB and one of the bestWe use this often in WB and one of the best antibodies out there, gives clean band in WB consistently.
Date published: 2015-09-09
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FGFR-3 抗体 (B-9) is rated 4.1 out of 5 by 12.
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