Date published: 2025-9-8

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FAPα 抗体 (SS-13): sc-100528

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说明书
  • FAP alpha 抗体 SS-13 是小鼠单克隆 IgG2a κ,FAPα抗体, 在11篇文献中引用,规格为100 µg/ml
  • 针对部分重组蛋白的抗体,可识别FAPα的内部区域,human来源
  • 推荐用于 human 来源的FAPα WB, IP 和 ELISA检测
  • 关于如何获取FAPα (SS-13): sc-100528免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP是FAPα Antibody (SS-13) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与FAPα Antibody (SS-13) 打包提供(请参阅下面的订购信息)。

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関連項目

FAPα抗体(SS-13)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和酶联免疫吸附测定(ELISA)检测人类FAPα蛋白。抗FAPα抗体(SS-13)以非结合形式提供。FAPα,也称为Seprase,是一种由人类基因FAP编码的760个氨基酸的蛋白质,是蛋白酶S9B家族的一员。FAPα(SS-13)抗体针对一种在组织重塑中发挥关键作用的蛋白质,特别是在发育和伤口愈合过程中,因为FAPα可能有助于恶性肿瘤的侵袭性,所以这一点非常重要。FAPα可降解明胶和热变性I型和IV型胶原蛋白,但不会分解天然I型和IV型胶原蛋白、层粘连蛋白、纤维粘连蛋白、纤维蛋白或酪蛋白。FAPα是一种单通道II型膜蛋白,位于细胞表面的板状足、侵入足和脱落小泡上,通常以与DPP4结合的糖基化同二聚体或异二聚体的形式存在。FAPα的单体形式不具有活性,这凸显了二聚体对于功能活性的重要性。这种独特的结构特征凸显了FAPα在促进与其他蛋白相互作用方面的作用,以及其在癌症生物学中的潜在意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FAPα 抗体 (SS-13) 参考文献:

  1. 人成纤维细胞活化蛋白α底物特异性的结构和动力学分析。  |  Aertgeerts, K., et al. 2005. J Biol Chem. 280: 19441-4. PMID: 15809306
  2. 成纤维细胞活化蛋白-α 和二肽基肽酶 IV (CD26):激活细胞信号的细胞表面蛋白酶,是癌症治疗的潜在靶点。  |  Kelly, T. 2005. Drug Resist Updat. 8: 51-8. PMID: 15939342
  3. 癌症基质标志物的特征:对成纤维细胞活化蛋白和内糖蛋白的 mRNA 表达数据库进行硅学分析。  |  Dolznig, H., et al. 2005. Cancer Immun. 5: 10. PMID: 16076089
  4. 膜型丝氨酸蛋白酶 seprase 的表达增加与人类结直肠癌的淋巴结转移缔合作用'。  |  Iwasa, S., et al. 2005. Cancer Lett. 227: 229-36. PMID: 16196122
  5. 成纤维细胞活化蛋白α在受到促炎刺激后由软骨细胞表达,在骨关节炎中会升高。  |  Milner, JM., et al. 2006. Arthritis Res Ther. 8: R23. PMID: 16507127
  6. 二肽脯氨酸二苯基膦酸盐是一种强效, 不可逆的胰蛋白酶(FAPalpha)抑制剂。  |  Gilmore, BF., et al. 2006. Biochem Biophys Res Commun. 346: 436-46. PMID: 16769036
  7. 减数分裂:分离酶两次罢工。  |  Terret, ME. and Jallepalli, PV. 2006. Nat Cell Biol. 8: 910-1. PMID: 16946739
  8. 上皮性卵巢癌患者流出物中 seprase 的表达。  |  Zhang, MZ., et al. 2007. Chin Med J (Engl). 120: 663-8. PMID: 17517181
  9. Simlukafusp Alfa(FAP-IL2v)联合或不联合阿特珠单抗治疗日本晚期实体瘤患者的 I 期研究。  |  Koyama, T., et al. 2024. Cancer Res Commun. 4: 2349-2358. PMID: 39140264
  10. 成纤维细胞活化蛋白-α的酶活性对TGF-β1诱导的人牙周韧带细胞成纤维细胞分化至关重要。  |  Kim, SM. and Jang, YJ. 2024. Exp Cell Res. 442: 114230. PMID: 39222867
  11. 针对成纤维细胞活化蛋白-α的高效α和β放射性核素疗法在侵袭性临床前小鼠肿瘤模型中的应用。  |  Ceuppens, H., et al. 2024. Eur J Nucl Med Mol Imaging.. PMID: 39237746
  12. FAP-α是评估肺腺癌基质侵袭的有效工具。  |  Xiong, S., et al. 2024. Diagn Pathol. 19: 152. PMID: 39587644

订购信息

产品名称产品编号规格价格数量收藏夹

FAPα 抗体 (SS-13)

sc-100528
100 µg/ml
RMB2504.00

FAPα (SS-13): m-IgG Fc BP-HRP 套装

sc-539695
100 µg Ab; 10 µg BP
RMB2662.00

FAPα (SS-13): m-IgG2a BP-HRP 套装

sc-546757
100 µg Ab; 10 µg BP
RMB2662.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with FAPα (SS-13): sc-100528 antibody?

Asked by: jenniferc
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Great for Western BlotThis antibody detects strong bands in human cell lines HepG2 and Wi-38. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from It works very well in western blot analysisThe an)body is very good. It is specific and the lanes are very clear. At line 1: nega)ve control At line 2 : carcinoma associated fibroblasts (CAFs) My condi)ons are: Blocking with milk 5% 1h Primary an)body 1:500 tTBS on Secondary an)body: 1:3000 mouse 3% milk
Date published: 2019-09-18
Rated 4 out of 5 by from This antibody was used in a publication for ICCThis antibody was used in a publication for ICC, IP and WB on human mammary tissues and breast cancer cell lines - SCBT Publication Review
Date published: 2015-05-03
Rated 4 out of 5 by from WB and IHC publishable data with human OSWB and IHC publishable data with human OS cell lines - SCBT Publication Review
Date published: 2013-03-26
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FAPα 抗体 (SS-13) is rated 4.5 out of 5 by 4.
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