Date published: 2025-9-7
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FANCL Antibody (B-11) - Immunohistochemistry - Image 158177
FANCL 抗体 (B-11): sc-137067. 福尔马林固定, 石蜡包埋的人胆囊组织的免疫过氧化物酶染色显示腺细胞的胞浆和膜染色.
FANCL 抗体 (B-11): sc-137067. 福尔马林固定, 石蜡包埋的人胆囊组织的免疫过氧化物酶染色显示腺细胞的胞浆和膜染色.

FANCL 抗体 (B-11): sc-137067

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说明书
  • FANCL抗体(B-11)是小鼠单克隆IgG2b κ, 在5篇文献中引用，规格为200 µg/ml
  • 针对氨基酸76-375，在FANCL的human起源的C-terminus处进行映射
  • 抗-FANCL 抗体 (B-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 FANCL
  • 抗FANCL抗体（B-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FANCL (B-11): sc-137067的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 FANCL 抗体 (B-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FANCL 抗体 (B-11) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 FANC 抗体 包括 FANCC, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCL和FANCM

FANCL抗体（B-11）是一种IgG2b κ小鼠单克隆FANCL抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人的FANCL。FANCL抗体（B-11）既可以是非偶联的抗FANCL抗体形式，也可以是多种偶联形式的抗FANCL抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。FANCL的缺陷是范可尼贫血（Fanconi anemia, FA）的原因之一。范可尼贫血是一种常染色体隐性遗传病，以骨髓衰竭、出生缺陷和染色体不稳定性为特征。在细胞水平上，范可尼贫血以自发性染色体断裂和对DNA交联剂的独特超敏感性为特征。目前已鉴定出至少8个互补群，并克隆了6个范可尼贫血基因（A、C、D2、E、F和G亚型）。FANC（范可尼贫血互补群）蛋白的磷酸化对范可尼贫血通路的功能很重要。范可尼贫血蛋白在共同通路中协同作用，最终使FANCD2蛋白单泛素化，并使FANCD2和BRCA1蛋白在核焦点中共定位。FANCL是一种连接酶蛋白，介导FANCD2的泛素化，这是DNA损伤通路中的关键步骤。FANCL可能对胚胎阶段原始生殖细胞的适当增殖是必需的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FANCL 抗体 (B-11) 参考文献:

  1. 范可尼贫血症缺乏一种新型泛素连接酶  |  Meetei, AR., et al. 2003. Nat Genet. 35: 165-70. PMID: 12973351
  2. 范可尼贫血症核心复合体在 S 期与染色质结合。  |  Mi, J. and Kupfer, GM. 2005. Blood. 105: 759-66. PMID: 15256425
  3. 范可尼贫血症补体 B 组的 X 连锁遗传。  |  Meetei, AR., et al. 2004. Nat Genet. 36: 1219-24. PMID: 15502827
  4. 阿什肯纳兹犹太人的范可尼贫血症。  |  Kutler, DI. and Auerbach, AD. 2004. Fam Cancer. 3: 241-8. PMID: 15516848
  5. 范可尼贫血症 DNA 损伤反应网络和 BRCA 蛋白的新进展。FAAP95 取代 BRCA2 成为真正的 FANCB 蛋白。  |  Fei, P., et al. 2005. Cell Cycle. 4: 80-6. PMID: 15611632
  6. 人类古DNA修复蛋白Hef的直向同源物在范可尼贫血症互补组M中存在缺陷。  |  Meetei, AR., et al. 2005. Nat Genet. 37: 958-63. PMID: 16116422

订购信息

产品名称产品编号规格价格数量收藏夹

FANCL 抗体 (B-11)

sc-137067
200 µg/ml
RMB2377.00

FANCL (B-11): m-IgGκ BP-HRP 套装

sc-521413
200 µg Ab, 40 µg BP
RMB2662.00

FANCL (B-11): m-IgG2b BP-HRP 套装

sc-548891
200 µg Ab; 10 µg BP
RMB2662.00

FANCL 抗体 (B-11) AC

sc-137067 AC
500 µg/ml, 25% agarose
RMB3129.00

FANCL 抗体 (B-11) HRP

sc-137067 HRP
200 µg/ml
RMB2377.00

FANCL 抗体 (B-11) FITC

sc-137067 FITC
200 µg/ml
RMB2482.00

FANCL 抗体 (B-11) PE

sc-137067 PE
200 µg/ml
RMB2580.00

FANCL 抗体 (B-11) Alexa Fluor® 488

sc-137067 AF488
200 µg/ml
RMB2685.00

FANCL 抗体 (B-11) Alexa Fluor® 546

sc-137067 AF546
200 µg/ml
RMB2685.00

FANCL 抗体 (B-11) Alexa Fluor® 594

sc-137067 AF594
200 µg/ml
RMB2685.00

FANCL 抗体 (B-11) Alexa Fluor® 647

sc-137067 AF647
200 µg/ml
RMB2685.00

FANCL 抗体 (B-11) Alexa Fluor® 680

sc-137067 AF680
200 µg/ml
RMB2685.00

FANCL 抗体 (B-11) Alexa Fluor® 790

sc-137067 AF790
200 µg/ml
RMB2685.00

For FANCL western blot from whole cell lysate what dilution of antibody should be used? Also please tell how long it should be incubated. I used antibody at a dilution of 1:500 and incubated for 4 hours at RT. I am getting multiple specific bands.

Asked by: Gaurav Joshi
Thank you for your question. We recommend using this antibody initially at the starting dilution of 1:200 (dilution range 1:100-1:1,000). We recommend incubating for 1 hour at room temperature in 5% nonfat milk + TBST. Please refer to our protocol online for more detailed information.
Answered by: Technical Support
Date published: 2019-01-31

I am using FANCL (B-11): sc-137067 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent choice for FANCL detection!Great antibody for IHC on paraffin embedded tissue sections with nice clean staining. Also works great for IF.
Date published: 2017-01-31
Rated 4 out of 5 by from Worked well in WB with human samplesWorked well in WB with human samples
Date published: 2015-10-12
Rated 5 out of 5 by from Strong band detected in WB in human FANCLStrong band detected in WB in human FANCL transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Robust signal in WB in human FANCL transfectedRobust signal in WB in human FANCL transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-11
Rated 5 out of 5 by from Published data for IP and WB in Hela cellsPublished data for IP and WB in Hela cells. -SCBT Publication Review
Date published: 2015-03-19
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic andExcellent immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-03-16
Rated 5 out of 5 by from Published data for IP and WB in human KSL cellsPublished data for IP and WB in human KSL cells. -SCBT Publication Review
Date published: 2015-02-03
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FANCL 抗体 (B-11) is rated 4.9 out of 5 by 7.
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