Date published: 2025-9-7
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FANCI Antibody (A-7) - Western Blotting - Image 360057
FANCI 抗体 (A-7) HRP: sc-271316 HRP. U-2 OS (A), MDA-MB-231 (B), Raji (C), HeLa (D), Daudi (E) 和 GA-10 (F) 全细胞裂解液中 FANCI 表达的直接 Western 印迹分析.
FANCI 抗体 (A-7) HRP: sc-271316 HRP. U-2 OS (A), MDA-MB-231 (B), Raji (C), HeLa (D), Daudi (E) 和 GA-10 (F) 全细胞裂解液中 FANCI 表达的直接 Western 印迹分析.

FANCI 抗体 (A-7): sc-271316

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寫評論提問

说明书
  • FANCI 抗体 A-7 是小鼠单克隆 IgG1 κ，FANCI抗体, 在15篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1002-1103位于human物种的FANCI的C-terminus附近
  • FANCI 抗体 (A-7) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 FANCI
  • 抗FANCI抗体（A-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FANCI (A-7): sc-271316的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是FANCI Antibody (A-7) for WB applications. 的首选辅助检测试剂，这些试剂现与FANCI Antibody (A-7) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外FANC抗体，包括FANCC, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCL和FANCM

FANCI抗体（A-7）是一种IgG1 κ小鼠单克隆FANCI抗体（也称为FANCI抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测人类来源的FANCI蛋白。FANCI抗体（A-7）既有非偶联形式的抗FANCI抗体，也有多种偶联形式的抗FANCI抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。范可尼贫血（FA）是一种常染色体隐性遗传病，其特征是骨髓衰竭、出生缺陷和染色体不稳定。在细胞水平上，FA的特征是自发性的染色体断裂和对DNA交联剂的独特超敏反应。FANCI（范可尼贫血，互补组I），也称为KIAA1794，是一种由1328个氨基酸组成的蛋白质，定位于细胞核，并受到单泛素化和DNA损伤依赖性磷酸化的影响。FANCI直接与FANCD2相互作用，是维持染色体稳定所必需的，还参与DNA双链断裂和DNA交联反应中的DNA重组和修复。编码FANCI的基因缺陷与FA的发病机理有关。FANCI以四种选择性剪接异构体的形式表达，并由一个基因编码，该基因定位于人类15号染色体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FANCI 抗体 (A-7) 参考文献:

  1. 未知人类基因编码序列的预测。XX.100 个新的大脑 cDNA 克隆的完整序列，它们在体外编码大型蛋白质。  |  Nagase, T., et al. 2001. DNA Res. 8: 85-95. PMID: 11347906
  2. 鉴定 FANCI 蛋白，它是 DNA 修复所需的单泛素化 FANCD2 旁系亲属。  |  Smogorzewska, A., et al. 2007. Cell. 129: 289-301. PMID: 17412408
  3. 确定范可尼贫血症互补 I 组基因 FANCI。  |  Dorsman, JC., et al. 2007. Cell Oncol. 29: 211-8. PMID: 17452773
  4. FANCI 是范可尼贫血症通路的第二个单泛素化成员。  |  Sims, AE., et al. 2007. Nat Struct Mol Biol. 14: 564-7. PMID: 17460694
  5. FANCI 磷酸化是开启范可尼贫血症通路的分子开关。  |  Ishiai, M., et al. 2008. Nat Struct Mol Biol. 15: 1138-46. PMID: 18931676
  6. Ube2t, FANCL 和 FANCI 对 FANCD2 的定点限制性单泛素化的机制研究。  |  Alpi, AF., et al. 2008. Mol Cell. 32: 767-77. PMID: 19111657
  7. 对FANCI-FANCD2单泛素化的结构研究。  |  Li, L., et al. 2020. Essays Biochem. 64: 807-817. PMID: 32725171
  8. FANCD2-FANCI泛素化和去泛素化的机制、特异性和功能。  |  Lemonidis, K., et al. 2022. FEBS J. 289: 4811-4829. PMID: 34137174
  9. FANCI可作为宫颈癌的预后生物标志物：系统评价和荟萃分析。  |  Liu, X., et al. 2021. Medicine (Baltimore). 100: e27690. PMID: 34941027
  10. FANCI/FANCD2复合物通过SRSF1介导的mRNA出口将DNA损伤反应与R-loop调节联系起来。  |  Olazabal-Herrero, A., et al. 2024. Cell Rep. 43: 113610. PMID: 38165804
  11. FANCI抑制诱导PARP1重新分布，从而提高PARP抑制剂在乳腺癌中的疗效。  |  Huang, YZ., et al. 2024. Cancer Res. 84: 3447-3463. PMID: 39037758
  12. FANCD2-FANCI检测DNA并识别双链到单链的连接点。  |  Alcón, P., et al. 2024. Nature. 632: 1165-1173. PMID: 39085614

订购信息

产品名称产品编号规格价格数量收藏夹

FANCI 抗体 (A-7)

sc-271316
200 µg/ml
RMB2377.00

FANCI (A-7): m-IgG Fc BP-HRP 套装

sc-527232
200 µg Ab; 10 µg BP
RMB2662.00

FANCI (A-7): m-IgG1 BP-HRP 套装

sc-532605
200 µg Ab; 20 µg BP
RMB2662.00

FANCI 抗体 (A-7) AC

sc-271316 AC
500 µg/ml, 25% agarose
RMB3129.00

FANCI 抗体 (A-7) HRP

sc-271316 HRP
200 µg/ml
RMB2377.00

FANCI 抗体 (A-7) FITC

sc-271316 FITC
200 µg/ml
RMB2482.00

FANCI 抗体 (A-7) PE

sc-271316 PE
200 µg/ml
RMB2580.00

FANCI 抗体 (A-7) Alexa Fluor® 488

sc-271316 AF488
200 µg/ml
RMB2685.00

FANCI 抗体 (A-7) Alexa Fluor® 546

sc-271316 AF546
200 µg/ml
RMB2685.00

FANCI 抗体 (A-7) Alexa Fluor® 594

sc-271316 AF594
200 µg/ml
RMB2685.00

FANCI 抗体 (A-7) Alexa Fluor® 647

sc-271316 AF647
200 µg/ml
RMB2685.00

FANCI 抗体 (A-7) Alexa Fluor® 680

sc-271316 AF680
200 µg/ml
RMB2685.00

FANCI 抗体 (A-7) Alexa Fluor® 790

sc-271316 AF790
200 µg/ml
RMB2685.00

这个抗体可以检测到FANCDI的单泛素化吗?

Asked by: XXXXXX
Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Yanru Tao
Date published: 2022-06-24

Can this antibody detect mouse FANCI?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 4
Date published: 2017-01-13

Does this antibody have an associated blocking peptide?

Asked by: AbPolly
Thank you for your question. As this antibody was raised against a recombinant protein and not a short peptide sequence, it is not possible to offer a blocking peptide for this antibody.
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 5 out of 5 by from Great features!I did Westernblot for human and chicken FANCI. It worked very well for human but not for chicken.
Date published: 2019-06-17
Rated 5 out of 5 by from Great features.I got the expected protein band at 7% SDS-PAGE Western blotting.
Date published: 2017-08-12
Rated 5 out of 5 by from OutstandingVery nice western data; strong signal and low background
Date published: 2017-01-23
Rated 5 out of 5 by from Good WB resultsThis FANCI antibody shows good results in WB for human samples.
Date published: 2017-01-14
Rated 5 out of 5 by from work wellthe antibody worked well in human lung cancer cell lysate on the dilution of 1:200.
Date published: 2016-11-02
Rated 5 out of 5 by from Works great in Western blot applicationsWorks great in Western blot applications! Strong bands in Raji and HeLa whole cell lysates. -SCBT QC
Date published: 2015-05-02
Rated 5 out of 5 by from Produced great immunofluorescence nuclearProduced great immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-17
Rated 5 out of 5 by from WB single clean banding using UWB single clean banding using U-2 OS whole cell lysate. -SCBT QC
Date published: 2015-01-04
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FANCI 抗体 (A-7) is rated 5.0 out of 5 by 8.
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