Date published: 2025-9-7
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FANCD2 Antibody (FI17) - Western Blotting - Image 375665
FANCD2 抗体 (FI17): sc-20022. HeLa (A), MCF7 (B), K-562 (C) 和 HL-60 (D) 核提取物以及 NTERA-2 cl. D1 (E) 和 Jurkat (F) 全细胞裂解液中 FANCD2 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
FANCD2 抗体 (FI17): sc-20022. HeLa (A), MCF7 (B), K-562 (C) 和 HL-60 (D) 核提取物以及 NTERA-2 cl. D1 (E) 和 Jurkat (F) 全细胞裂解液中 FANCD2 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

FANCD2 抗体 (FI17): sc-20022

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说明书
  • FANCD2 抗体 FI17 是小鼠单克隆 IgG1 κ，FANCD2抗体, 在202篇文献中引用，规格为200 µg/ml
  • 针对FANCD2的human起源的N-terminus的
  • FANCD2 抗体 (FI17) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 S and L isoforms of FANCD2
  • 抗FANCD2抗体（FI17）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FANCD2 (FI17): sc-20022的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 FANCD2 抗体 (FI17) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FANCD2 抗体 (FI17) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 FANC 抗体 包括 FANCC, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCL和FANCM

FANCD2抗体（FI17）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测人类源性FANCD2蛋白。抗FANCD2抗体（FI17）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。FANCD2在DNA损伤反应中发挥着关键作用，特别是在修复链间交联方面，这种DNA损伤如果得不到适当修复，会导致基因组不稳定和癌症。FANCD2是范可尼贫血（FA）通路的一部分，该通路是维持基因组完整性的关键细胞机制。当DNA受损时，FANCD2会经历单泛素化，这对于激活和随后定位到DNA受损部位至关重要，在此过程中，FANCD2与其他FA蛋白和乳腺癌易感蛋白BRCA1相互作用。这种相互作用对于将修复因子招募到受损DNA至关重要，从而促进有效的修复过程。人类FANCD2基因位于3p25.3染色体上，由44个外显子组成，编码一种1451个氨基酸的核蛋白，该蛋白有两种同工型，这凸显了其在细胞对DNA损伤的反应中的复杂性和重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FANCD2 抗体 (FI17) 参考文献:

  1. 范可尼贫血症蛋白 FANCF 与 FANCA, FANCC 和 FANCG 形成核复合体。  |  de Winter, JP., et al. 2000. Hum Mol Genet. 9: 2665-74. PMID: 11063725
  2. 范可尼贫血症与 DNA 修复  |  Grompe, M. and D'Andrea, A. 2001. Hum Mol Genet. 10: 2253-9. PMID: 11673408
  3. 一种细胞质丝氨酸蛋白激酶与范可尼贫血症蛋白 FANCA 结合，并可能对其进行调控。  |  Yagasaki, H., et al. 2001. Blood. 98: 3650-7. PMID: 11739169
  4. 范可尼贫血症补体F组和D1组细胞中范可尼贫血症通路的功能  |  Siddique, MA., et al. 2001. Exp Hematol. 29: 1448-55. PMID: 11750104
  5. 中国仓鼠 FANCG/XRCC9 突变体 NM3 不能表达 FANCD2 蛋白的单泛素化形式，对一系列 DNA 损伤剂不敏感，并表现出正常水平的自发姐妹染色单体交换。  |  Wilson, JB., et al. 2001. Carcinogenesis. 22: 1939-46. PMID: 11751423
  6. 范可尼贫血症蛋白 FANCA 的磷酸化受 Akt 激酶调控。  |  Otsuki, T., et al. 2002. Biochem Biophys Res Commun. 291: 628-34. PMID: 11855836
  7. 对FANCI-FANCD2单泛素化的结构研究。  |  Li, L., et al. 2020. Essays Biochem. 64: 807-817. PMID: 32725171
  8. FBXL12降解FANCD2，以调节复制恢复，并在复制压力条件下促进癌细胞存活。  |  Brunner, A., et al. 2023. Mol Cell. 83: 3720-3739.e8. PMID: 37591242
  9. FANCD2依赖的DNA合成依赖于PCNA K164的泛素化。  |  Leung, W., et al. 2023. Cell Rep. 42: 113523. PMID: 38060446
  10. FANCI/FANCD2复合物通过SRSF1介导的mRNA出口将DNA损伤反应与R-loop调节联系起来。  |  Olazabal-Herrero, A., et al. 2024. Cell Rep. 43: 113610. PMID: 38165804
  11. FANCD2缺陷通过铁死亡使SHH髓母细胞瘤对放疗敏感。  |  Zhou, H., et al. 2024. J Pathol. 262: 427-440. PMID: 38229567
  12. FANCD2-FANCI检测DNA并识别双链到单链的连接点。  |  Alcón, P., et al. 2024. Nature. 632: 1165-1173. PMID: 39085614

订购信息

产品名称产品编号规格价格数量收藏夹

FANCD2 抗体 (FI17)

sc-20022
200 µg/ml
RMB2377.00

FANCD2 (FI17): m-IgG Fc BP-HRP 套装

sc-528351
200 µg Ab; 10 µg BP
RMB2662.00

FANCD2 (FI17): m-IgGκ BP-HRP 套装

sc-520706
200 µg Ab, 40 µg BP
RMB2662.00

FANCD2 (FI17): m-IgG1 BP-HRP 套装

sc-542885
200 µg Ab; 20 µg BP
RMB2662.00

FANCD2 抗体 (FI17) AC

sc-20022 AC
500 µg/ml, 25% agarose
RMB3129.00

FANCD2 抗体 (FI17) HRP

sc-20022 HRP
200 µg/ml
RMB2377.00

FANCD2 抗体 (FI17) FITC

sc-20022 FITC
200 µg/ml
RMB2482.00

FANCD2 抗体 (FI17) PE

sc-20022 PE
200 µg/ml
RMB2580.00

FANCD2 抗体 (FI17) Alexa Fluor® 488

sc-20022 AF488
200 µg/ml
RMB2685.00

FANCD2 抗体 (FI17) Alexa Fluor® 546

sc-20022 AF546
200 µg/ml
RMB2685.00

FANCD2 抗体 (FI17) Alexa Fluor® 594

sc-20022 AF594
200 µg/ml
RMB2685.00

FANCD2 抗体 (FI17) Alexa Fluor® 647

sc-20022 AF647
200 µg/ml
RMB2685.00

FANCD2 抗体 (FI17) Alexa Fluor® 680

sc-20022 AF680
200 µg/ml
RMB2685.00

FANCD2 抗体 (FI17) Alexa Fluor® 790

sc-20022 AF790
200 µg/ml
RMB2685.00

Is this a purified antibody? What method used for purification

Asked by: Rash
Antibodies raised against recombinant immunogens are not affinity purified. They are purified using a proprietary method.
Answered by: Technical Service
Date published: 2024-10-11

Has this antibody been validated by FANCD2 KO or KD for immunostaining?

Asked by: JS001
Thank you for your question. We recommend the antibody for WB, IP,and PAR.
Answered by: BlakeJ
Date published: 2023-08-11

Will this antibody give me both the ubiquinated and non-ubiquinated versions of FANCD2. Treatment with cisplatin does not seem to work to give the 2 bands needed (switched cisplatin as well)

Asked by: CalT
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Has anyone used this antibody for ChIP-PCR or ChIP-seq? The citations don't seem to be searchable.

Asked by: aprilmrning
Thank you for your question. We are not aware of any publications citing use of this antibody, FANCD2 (FI17): sc-20022, for ChIP. In the future, you can search our product citations for specific applications using the Product Citation Search feature found at the bottom of the page. A link below is provided as a reference. https://www.scbt.com/scbt/misc/citationSearch.jsp
Answered by: Technical Support
Date published: 2017-07-18

How can this antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: AbPolly
Thank you for your inquiry. To use more than one primary antibody from the same host in staining, it is recommended to have those antibodies directly conjugated to avoid cross-reaction of secondary antibodies. This FANCD2 antibody is available conjugated to HRP, PE, FITC, AF 488 and AF 647.
Answered by: Technical Service
Date published: 2016-12-20

What is the recommended secondary reagent for this primary antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service
Date published: 2016-12-20
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Rated 5 out of 5 by from WB analysis of FANCD2 antibodyWB analysis of FANCD2 using FANCD2 Antibody (FI17) gave me clean and specific images.
Date published: 2020-08-07
Rated 4 out of 5 by from WBA strong and specific band was achieved in hepatoma cancer cell cell lysates.
Date published: 2018-12-24
Rated 4 out of 5 by from Good antibodyAntibody was used at 1:1000 from A431 extracts and met expectations.
Date published: 2018-05-29
Rated 4 out of 5 by from Great AntibodyI use this antibody regularly in different breast cancer cell lines for western blot. It works great ( 1 in 1000, 2%milk-TBST). However, I find some batches to be weaker than others. So I increase the concentration to 1 in 750 to get a strong signal. I don't recommend to use it for immunocytochemistry as I tried as low as 1:50 but the signal is extremely weak.
Date published: 2017-06-29
Rated 4 out of 5 by from no revGood product which met our expectation as far as Western blot was concerned.
Date published: 2017-06-15
Rated 5 out of 5 by from Great, multi-function antibodyThe FANCD2 Antibody (FI17) is a functionally diverse antibody with nearly 50 publication citations for applications including Western blot, immunofluorescence and immunohistochemistry.
Date published: 2016-12-23
Rated 4 out of 5 by from Use 1:200Gives good and specific signal for both unmodified and mono-ubiquitinated Fancd2 on WB (use no less than 1:200).
Date published: 2016-12-16
Rated 5 out of 5 by from work wellthe dilution is 1:200, and the antibody worked well in A549 cell lysate.
Date published: 2016-11-02
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FANCD2 抗体 (FI17) is rated 4.7 out of 5 by 15.
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