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Factor H Antibody (H-5) - Western Blotting - Image 63197
因子 H 抗体 (H-5): sc-166608. 人 PBL 全细胞裂解液中因子 H 表达的 Western 印迹分析.
因子 H 抗体 (H-5): sc-166608. 人 PBL 全细胞裂解液中因子 H 表达的 Western 印迹分析.

Factor H 抗体 (H-5): sc-166608

4.7(7)
寫評論提問

说明书
  • Factor H抗体(H-5)是小鼠单克隆IgG1 κ, 在6篇文献中引用，规格为200 µg/ml
  • 针对氨基酸61-360的图谱，在Factor H的内部区域，human起源
  • Factor H 抗体 (H-5) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 Factor H
  • 抗Factor H抗体（H-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Factor H (H-5): sc-166608的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Factor H Antibody (H-5) for WB applications. 的首选辅助检测试剂，这些试剂现与Factor H Antibody (H-5) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Coagulation Factor抗体，包括Factor B, Factor H, Factor I, Factor IX, Factor V, Factor VII, Factor VIII, Factor X, Factor XI, Factor XII和Factor XIII

因子H抗体（H-5）是一种IgG1 κ小鼠单克隆因子H抗体，可通过WB、IP、IF和ELISA检测人类来源的因子H。因子H抗体（H-5）既可作为非偶联的抗因子H抗体形式，也可作为多种偶联形式的抗因子H抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。因子H基因家族是一个多域、多功能蛋白质家族，其各个成员由保守的结构元素定义，显示出多样但经常重叠的功能。这些蛋白质共享一个共同的结构基序，即短共识重复序列（SCR），其在相关基因和系统发育不同的物种之间在结构上都是保守的。人类补体因子H（FH、CFH、HUS、b-1H）基因编码一个由1213个氨基酸组成的血清糖蛋白，该糖蛋白被排列成20个SCRs，每个SCR大约由60个氨基酸组成，以及一个由18个残基组成的先导序列。因子H控制替代补体途径的功能，并作为与因子I（C3b失活剂）的辅助因子。此外，因子H在补体系统外具有功能活性，在那里它可以与细胞整合素受体（CD11b/CD18）结合，与细胞表面糖胺聚糖相互作用，并与某些致病微生物的表面结合。因子H缺乏是急性肾病的常见特征。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Factor H 抗体 (H-5) 参考文献:

  1. 人类补体因子H：功能作用、遗传变异和疾病关联。  |  Rodríguez de Córdoba, S., et al. 2004. Mol Immunol. 41: 355-67. PMID: 15163532
  2. 补体因子H的翻译小综述系列：与补体因子H相关的肾脏疾病：来自人类和动物的新见解。  |  Pickering, MC. and Cook, HT. 2008. Clin Exp Immunol. 151: 210-30. PMID: 18190458
  3. 补体因子H相关蛋白与免疫疾病的关系。  |  Skerka, C. and Zipfel, PF. 2008. Vaccine. 26 Suppl 8: I9-14. PMID: 19388158
  4. 因子h和普鲁定识别肾小管上皮肝素硫酸酯的不同表位。  |  Zaferani, A., et al. 2012. J Biol Chem. 287: 31471-81. PMID: 22815489
  5. 因子H和血管性血友病因子之间的相互作用。  |  Feng, S., et al. 2013. PLoS One. 8: e73715. PMID: 23991205
  6. 一种扩展的迷你补体因子H分子可改善实验性C3肾小球病。  |  Nichols, EM., et al. 2015. Kidney Int. 88: 1314-1322. PMID: 26221753
  7. 小鼠因子H相关蛋白FHR-B促进补体激活。  |  Cserhalmi, M., et al. 2017. Front Immunol. 8: 1145. PMID: 28974948
  8. 补体因子H缺陷小鼠可自发形成肝肿瘤。  |  Laskowski, J., et al. 2020. J Clin Invest. 130: 4039-4054. PMID: 32369457
  9. 补体C3d蛋白的肽生物标志物设计受因子H启发。  |  Harrison, RES., et al. 2020. ACS Med Chem Lett. 11: 1054-1059. PMID: 32435425
  10. 常见的补体因子H多态性与老年患者的牙周炎有关。  |  Salminen, A., et al. 2022. J Periodontol. 93: 1626-1634. PMID: 35315933

订购信息

产品名称产品编号规格价格数量收藏夹

Factor H 抗体 (H-5)

sc-166608
200 µg/ml
RMB2377.00

Factor H (H-5): m-IgG Fc BP-HRP 套装

sc-527173
200 µg Ab; 10 µg BP
RMB2662.00

Factor H (H-5): m-IgG1 BP-HRP 套装

sc-532546
200 µg Ab; 20 µg BP
RMB2662.00

Factor H 抗体 (H-5) AC

sc-166608 AC
500 µg/ml, 25% agarose
RMB3129.00

Factor H 抗体 (H-5) HRP

sc-166608 HRP
200 µg/ml
RMB2377.00

Factor H 抗体 (H-5) FITC

sc-166608 FITC
200 µg/ml
RMB2482.00

Factor H 抗体 (H-5) PE

sc-166608 PE
200 µg/ml
RMB2580.00

Factor H 抗体 (H-5) Alexa Fluor® 488

sc-166608 AF488
200 µg/ml
RMB2685.00

Factor H 抗体 (H-5) Alexa Fluor® 546

sc-166608 AF546
200 µg/ml
RMB2685.00

Factor H 抗体 (H-5) Alexa Fluor® 594

sc-166608 AF594
200 µg/ml
RMB2685.00

Factor H 抗体 (H-5) Alexa Fluor® 647

sc-166608 AF647
200 µg/ml
RMB2685.00

Factor H 抗体 (H-5) Alexa Fluor® 680

sc-166608 AF680
200 µg/ml
RMB2685.00

Factor H 抗体 (H-5) Alexa Fluor® 790

sc-166608 AF790
200 µg/ml
RMB2685.00

What blocking solution should I use with Factor H (H-5): sc-166608 monoclonal antibody for Western blot?

Asked by: AbPolly
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Works for IHCReceived a sample to test for IHC. Worked in both Human and rabbit. Used Citrate for retrieval and incubated at 10ug/mL overnight at 4C.
Date published: 2017-11-13
Rated 3 out of 5 by from No positive signal in IF in rat's brain sectionI think there is no positive signal in IF in SD rat's brain frozen section by competing the image with that of negative control. I did atigen retrive by heat in 95 ° citrate buffer solution (pH 6) before the staining.
Date published: 2017-08-15
Rated 5 out of 5 by from Good publishable data in WB in human HNGood publishable data in WB in human HN-brain cells. -SCBT Publication Review
Date published: 2015-05-05
Rated 5 out of 5 by from Positive signal in WB in recombinant factorPositive signal in WB in recombinant factor H fusion protein. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Worked well in WB in human and mouse brainWorked well in WB in human and mouse brain and retinal tissues. -SCBT Publication Review
Date published: 2015-02-14
Rated 5 out of 5 by from Good specificity in WB in human brainGood specificity in WB in human brain, liver and spleen tissue. -SCBT Publication Review
Date published: 2015-01-17
Rated 5 out of 5 by from Robust signal in WB in human PBL whole cell lysateRobust signal in WB in human PBL whole cell lysate. -SCBT QC
Date published: 2013-06-22
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Factor H 抗体 (H-5) is rated 4.7 out of 5 by 7.
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