Date published: 2025-9-6
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ERK 2 Antibody (D-2) - Western Blotting - Image 396223
ERK 2 抗体 (D-2) Alexa Fluor® 790: sc-1647 AF790. 在 HeLa (A), NIH/3T3 (B), KNRK (C), Jurkat (D) 和 K-562 (E) 全细胞裂解液中对 ERK 2 表达的直接近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
ERK 2 抗体 (D-2) Alexa Fluor® 790: sc-1647 AF790. 在 HeLa (A), NIH/3T3 (B), KNRK (C), Jurkat (D) 和 K-562 (E) 全细胞裂解液中对 ERK 2 表达的直接近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

ERK 2 抗体 (D-2): sc-1647

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说明书
  • ERK 2抗体(D-2)是小鼠单克隆IgG2b κ, 在615篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的ERK 2 MAP kinase p42的C-terminus的氨基酸345-358之间
  • ERK 2 抗体 (D-2) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA ，检测 mouse, rat, human 和 来源的ERK 2 p42; 也和以下物种反应，包括： 和 canine, bovine, porcine and avian
  • 抗ERK 2抗体（D-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取ERK 2 (D-2): sc-1647的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 ERK 2 抗体 (D-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ERK 2 抗体 (D-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外ERK抗体，包括ERK 1, ERK 2, ERK 3和ERK 5

ERK 2抗体（D-2）是一种IgG2b κ小鼠单克隆ERK 2抗体（也称为ERK 2抗体或MAPK1抗体），可通过WB、IP、IF、IHC（P）、FCM和ELISA检测小鼠、大鼠和人类的ERK 2 p42。ERK 2抗体（D-2）可作为非偶联抗ERK 2抗体形式，以及多种偶联抗ERK 2抗体形式提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。丝裂原活化蛋白激酶（MAPK）信号通路涉及两种密切相关的MAP激酶，即细胞外信号相关激酶1（ERK 1，p44）和2（ERK 2，p42）。生长因子、类固醇激素、G蛋白偶联受体配体和神经递质可启动MAPK信号通路。ERK1和ERK2的激活需要上游激酶如MAP激酶激酶（MEK）、MEK激酶和Raf-1的磷酸化。ERK1和ERK2的磷酸化可发生在特定酪氨酸和苏氨酸位点，这些位点映射在包括苏氨酸-谷氨酸-酪氨酸基序在内的共有基序内。ERK的激活导致与其他ERKs的二聚化并随后定位到细胞核。活性ERK二聚体磷酸化核蛋白上的丝氨酸和苏氨酸残基，并影响一系列反应，包括增殖、分化、转录调控和发育。人类ERK2基因定位于22q11.21染色体，编码一个由360个氨基酸组成的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ERK 2 抗体 (D-2) 参考文献:

  1. G 蛋白偶联受体对丝裂原活化蛋白激酶信号网络的调控。  |  Gutkind, JS. 2000. Sci STKE. 2000: re1. PMID: 11752597
  2. MAP 激酶（ERKs）信号的保真度和时空控制。  |  Pouysségur, J., et al. 2002. Biochem Pharmacol. 64: 755-63. PMID: 12213567
  3. 人唾液腺粘液表皮样癌中细胞外信号调节的ERK-1/ERK-2通路激活：与侵袭性肿瘤行为和肿瘤细胞增殖有关。  |  Handra-Luca, A., et al. 2003. Am J Pathol. 163: 957-67. PMID: 12937136
  4. ERK1和ERK2是两种微管相关蛋白2激酶，它们在原位介导酪氨酸羟化酶丝氨酸-31的磷酸化。  |  Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
  5. MEK 的主要结构，MEK 是一种蛋白激酶，能使 ERK 基因产物磷酸化。  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  6. T 细胞中的细胞外信号调节激酶：人类 ERK1 和 ERK2 cDNA 的特征。  |  Owaki, H., et al. 1992. Biochem Biophys Res Commun. 182: 1416-22. PMID: 1540184
  7. ERK：丝氨酸/苏氨酸蛋白激酶家族，在胰岛素和 NGF 的作用下被激活并酪氨酸磷酸化。  |  Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
  8. 宿主细胞MAPK/ERK-2整合到慢病毒颗粒中的机制：MAPK/ERK-2与结构蛋白Gag的脯氨酸富集结构域之间的相互作用。  |  Gupta, P., et al. 2011. J Mol Biol. 410: 681-97. PMID: 21762808
  9. 追踪1,2,4-三取代咪唑啉酮P38 MAP激酶和ERK-2抑制剂的结合模式。  |  Rao, SN. 2017. J Mol Graph Model. 76: 161-171. PMID: 28743070
  10. 低强度脉冲超声刺激、体外冲击波和径向压力波对大鼠胫骨缺损骨愈合过程中Akt、BMP-2、ERK-2、FAK和TGF-β1的影响。  |  Buarque de Gusmão, CV., et al. 2019. Ultrasound Med Biol. 45: 2140-2161. PMID: 31101448
  11. 磷酸化 MAP 激酶 ERK2 可促进其同源二聚化和核转运。  |  Khokhlatchev, AV., et al. 1998. Cell. 93: 605-15. PMID: 9604935
  12. Sp1磷酸化作用通过ERK 2刺激DNA结合。  |  Merchant, JL., et al. 1999. Biochem Biophys Res Commun. 254: 454-61. PMID: 9918860

订购信息

产品名称产品编号规格价格数量收藏夹

ERK 2 抗体 (D-2)

sc-1647
200 µg/ml
RMB2377.00

ERK 2 (D-2): m-IgGκ BP-HRP 套装

sc-520370
200 µg Ab, 40 µg BP
RMB2662.00

ERK 2 (D-2): m-IgG2b BP-HRP 套装

sc-548827
200 µg Ab; 10 µg BP
RMB2662.00

ERK 2 抗体 (D-2) AC

sc-1647 AC
500 µg/ml, 25% agarose
RMB3129.00

ERK 2 抗体 (D-2) HRP

sc-1647 HRP
200 µg/ml
RMB2377.00

ERK 2 抗体 (D-2) FITC

sc-1647 FITC
200 µg/ml
RMB2482.00

ERK 2 抗体 (D-2) PE

sc-1647 PE
200 µg/ml
RMB2580.00

ERK 2 抗体 (D-2) Alexa Fluor® 488

sc-1647 AF488
200 µg/ml
RMB2685.00

ERK 2 抗体 (D-2) Alexa Fluor® 546

sc-1647 AF546
200 µg/ml
RMB2685.00

ERK 2 抗体 (D-2) Alexa Fluor® 594

sc-1647 AF594
200 µg/ml
RMB2685.00

ERK 2 抗体 (D-2) Alexa Fluor® 647

sc-1647 AF647
200 µg/ml
RMB2685.00

ERK 2 抗体 (D-2) Alexa Fluor® 680

sc-1647 AF680
200 µg/ml
RMB2685.00

ERK 2 抗体 (D-2) Alexa Fluor® 790

sc-1647 AF790
200 µg/ml
RMB2685.00

ERK 2 抗体 (D-2) B

sc-1647 B
200 µg/ml
RMB2407.00

ERK 2 (D-2) 中和勝肽

sc-1647 P
100 µg/0.5 ml
RMB511.00

Hi, does this ERK2 ab detect both unphosphorylated and MEK-phosphorylated ERK2?

Asked by: CharlesF
Thank you for your question. Yes, this is a total ERK 2 antibody and can detect both phosphorylated and un-phosphorylated ERK 2. Please refer to the Western blot data on our website for further evidence of this reactivity.
Answered by: Technical Support
Date published: 2019-05-20

What is the evidence that this mAb is actually specific to ERK2 and does *not* bind (or minimally binds) ERK1? The given immunizing sequence (ERK2 345-358) shares 12/13 amino acid residues with ERK1 so it is a little hard to believe this is ERK2-specific.

Asked by: Will Herrick
Thank you for your inquiry. Since we do not epitope map our monoclonal antibodies, we cannot say with certainty if this clone is cross-reactive with ERK 1, but there is a good possibility that it is reactive with ERK 1 p44.
Answered by: Technical Service
Date published: 2019-05-29
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Rated 5 out of 5 by from PerfectThis antibody works perfectly on HepG2 extracts analyzed by western blot
Date published: 2023-12-12
Rated 5 out of 5 by from ERK2 abThis antibody works perfectly in western blot analysis.
Date published: 2022-03-21
Rated 5 out of 5 by from Remarkable features!This antibody works great in western blot analysis in Rat Brain Astrocytes.
Date published: 2021-07-20
Rated 5 out of 5 by from ERK2 antibody (sc-1647)ERK2 antibody (sc-1647) was working very well in our Western blotting.
Date published: 2021-06-02
Rated 5 out of 5 by from Works well in western blotThis antibody works well in western blot of mouse liver samples
Date published: 2021-03-22
Rated 5 out of 5 by from ExcellentA great antibody for WB analysis with specific binding and a clear signal.
Date published: 2021-03-22
Rated 5 out of 5 by from ERK 2 Antibody (D-2)This antibody has worked well in West blotting.Excellent results.
Date published: 2020-10-27
Rated 5 out of 5 by from Good for WBWe used it at 1:500 dilution and it specifically detected endogenous human ERK2.
Date published: 2020-06-25
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ERK 2 抗体 (D-2) is rated 4.8 out of 5 by 31.
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