Date published: 2025-9-6

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ERK 2 抗体 (12A4): sc-81457

4.4(5)
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说明书
  • ERK 2 抗体 12A4 是小鼠单克隆 IgG1, 在26篇文献中引用,提供 50 µg/0.5 ml
  • 免疫human物种的ERK 2的氨基酸200-250
  • 推荐用于 mouse, rat 和 human 来源的ERK 2 WB 和 IP检测
  • 看 ERK 2 (D-2): sc-1647,可查看有标记的 ERK 2 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成ERK 2 抗体 (12A4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

丝裂原活化蛋白激酶(MAPK)信号通路涉及两种密切相关的MAP激酶,即细胞外信号相关激酶1(ERK 1,p44)和2(ERK 2,p42)。生长因子、类固醇激素、G蛋白偶联受体配体和神经递质可以启动MAPK信号通路。ERK 1和ERK 2的激活需要上游激酶如MAP激酶激酶(MEK)、MEK激酶和Raf-1的磷酸化。ERK 1和ERK 2的磷酸化可以发生在包含苏氨酸-谷氨酸-酪氨酸基序的一致基序内映射的特定酪氨酸和苏氨酸位点。ERK的激活导致与其他ERKs的二聚化,并随后定位到细胞核。活性ERK二聚体磷酸化核蛋白上的丝氨酸和苏氨酸残基,并影响一系列反应,包括增殖、分化、转录调控和发育。人类ERK 2基因映射到染色体22q11.21,并编码一个由360个氨基酸组成的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

ERK 2 抗体 (12A4)

sc-81457
50 µg/0.5 ml
RMB2377.00

I am using ERK 2 (12A4): sc-81457 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Germaine
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good data for WB in 293T and AGood data for WB in 293T and A-431 whole cell lysates. -SCBT QC
Date published: 2015-03-31
Rated 5 out of 5 by from Published with this antibody for WB in thePublished with this antibody for WB in the HER2 cells. -SCBT Publication Review
Date published: 2015-03-30
Rated 4 out of 5 by from Great antibody for WB in in serum starved AGreat antibody for WB in in serum starved A-431, A549, SKOV3 , OVCAR-5, HaCaT, PC-3, HeLa and Hep G2 whole cell lysates. -SCBT QC
Date published: 2015-02-23
Rated 5 out of 5 by from Good specificity and signal strength inGood specificity and signal strength in rat hippocampus tissue extract. -SCBT QC
Date published: 2015-02-17
Rated 3 out of 5 by from Worked well with transfected 293T whole cellWorked well with transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-08-17
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ERK 2 抗体 (12A4) is rated 4.4 out of 5 by 5.
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