Date published: 2025-9-7

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EphB4 抗体 (H-10): sc-365510

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说明书
  • EphB4 抗体 H-10 是小鼠单克隆 IgG1 κ,EphB4抗体, 在4篇文献中引用,规格为200 µg/ml
  • 与human的EphB4的细胞外结构域内的氨基酸201-400杂交
  • EphB4 抗体 (H-10) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 EphB4
  • 抗EphB4抗体(H-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EphB4 (H-10): sc-365510免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是EphB4 Antibody (H-10) for WB applications. 的首选辅助检测试剂,这些试剂现与EphB4 Antibody (H-10) 打包提供(请参阅下面的订购信息)。

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関連項目

EphB4抗体(H-10)是一种IgG1κ小鼠单克隆EphB4抗体(也被称为Eph受体B4抗体或HTK抗体),能够通过WB、IP、IF和ELISA方法检测人类来源的EphB4蛋白。EphB4抗体(H-10)以非偶联型抗EphB4抗体形式供应,同时也提供多种偶联型抗EphB4抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Eph亚家族是目前为止发现的最大的受体蛋白酪氨酸激酶群体。尽管这些受体的生物学活性尚未确定,但有越来越多的证据表明它们参与了中枢神经系统功能和发育过程。人类来源的Eph亚家族受体(及其小鼠/禽类同源物)包括EphA1(Eph)、EphA2(Eck)、EphA3(Hek4)、EphA4(Hek8)、EphA5(Hek7)、EphA6(Hek12)、EphA7(Hek11/MDK1)、EphA8(Hek3)、EphB1(Hek6)、EphB2(Hek5)、EphB3(Cek10,Hek2)、EphB4(Htk)、EphB5(Hek9)和EphB6(Mep)。Eph受体的配体包括ephrin-A4(LERK-4),它能够与EphA3和EphB1结合。此外,ephrin-A2(ELF-1)被描述为EphA4的配体,ephrin-A3(Ehk1-L)是EphA5的配体,而ephrin-B2(Htk-L)则是EphB4(Htk)的配体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EphB4 抗体 (H-10) 参考文献:

  1. EphB4在胶质瘤中过度表达,并促进胶质瘤细胞的生长。  |  Chen, T., et al. 2013. Tumour Biol. 34: 379-85. PMID: 23138393
  2. EphB4促进人类结直肠癌的增殖、侵袭和血管生成。  |  Lv, J., et al. 2016. Exp Mol Pathol. 100: 402-8. PMID: 27072105
  3. EphB4-VAV1信号通路与慢性髓细胞白血病细胞对伊马替尼的耐药性有关。  |  Zhang, JF., et al. 2016. Blood Cells Mol Dis. 59: 58-62. PMID: 27282569
  4. 环状EPHB4通过抑制HIF-1α在肝细胞癌中具有抗肿瘤作用。  |  Tan, Y., et al. 2019. Mol Carcinog. 58: 875-886. PMID: 30644610
  5. 血管生成取决于 EPHB4 介导的血管内皮细胞胶原蛋白 IV 的输出。  |  Chen, D., et al. 2022. JCI Insight. 7: PMID: 35015735
  6. ELF-1的鉴定和克隆,ELF-1是Mek4和Sek受体酪氨酸激酶的发育表达配体。  |  Cheng, HJ. and Flanagan, JG. 1994. Cell. 79: 157-68. PMID: 7522971
  7. EPH相关受体蛋白酪氨酸激酶Htk配体的分子克隆。  |  Bennett, BD., et al. 1995. Proc Natl Acad Sci U S A. 92: 1866-70. PMID: 7534404
  8. 鉴定 Eph 受体酪氨酸激酶家族新成员 Ehk-3 的全长和截短形式。  |  Valenzuela, DM., et al. 1995. Oncogene. 10: 1573-80. PMID: 7731712
  9. 鉴定编码 MDK1(一种在小鼠神经系统中表达的新型受体酪氨酸激酶)变体的替代剪接 mRNA。  |  Ciossek, T., et al. 1995. Oncogene. 10: 97-108. PMID: 7824284
  10. 受体酪氨酸激酶 hek 和 elk 的配体:分离编码蛋白质家族的 cDNA。  |  Kozlosky, CJ., et al. 1995. Oncogene. 10: 299-306. PMID: 7838529
  11. 五种人类 EPH 样受体蛋白-酪氨酸激酶的 cDNA 克隆和组织分布。  |  Fox, GM., et al. 1995. Oncogene. 10: 897-905. PMID: 7898931
  12. eph相关酪氨酸激酶受体配体家族的分子特征。  |  Beckmann, MP., et al. 1994. EMBO J. 13: 3757-62. PMID: 8070404

订购信息

产品名称产品编号规格价格数量收藏夹

EphB4 抗体 (H-10)

sc-365510
200 µg/ml
RMB2377.00

EphB4 (H-10): m-IgG Fc BP-HRP 套装

sc-527375
200 µg Ab; 10 µg BP
RMB2662.00

EphB4 (H-10): m-IgG1 BP-HRP 套装

sc-532748
200 µg Ab; 20 µg BP
RMB2662.00

EphB4 抗体 (H-10) AC

sc-365510 AC
500 µg/ml, 25% agarose
RMB3129.00

EphB4 抗体 (H-10) HRP

sc-365510 HRP
200 µg/ml
RMB2377.00

EphB4 抗体 (H-10) FITC

sc-365510 FITC
200 µg/ml
RMB2482.00

EphB4 抗体 (H-10) PE

sc-365510 PE
200 µg/ml
RMB2580.00

EphB4 抗体 (H-10) Alexa Fluor® 488

sc-365510 AF488
200 µg/ml
RMB2685.00

EphB4 抗体 (H-10) Alexa Fluor® 546

sc-365510 AF546
200 µg/ml
RMB2685.00

EphB4 抗体 (H-10) Alexa Fluor® 594

sc-365510 AF594
200 µg/ml
RMB2685.00

EphB4 抗体 (H-10) Alexa Fluor® 647

sc-365510 AF647
200 µg/ml
RMB2685.00

EphB4 抗体 (H-10) Alexa Fluor® 680

sc-365510 AF680
200 µg/ml
RMB2685.00

EphB4 抗体 (H-10) Alexa Fluor® 790

sc-365510 AF790
200 µg/ml
RMB2685.00

Does this only work for Human EphB4 or has it been tried in other species?

Asked by: Yale2019
Thank you for your question. This antibody has not yet been tested in any other species, so it is only recommended for human samples at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-08-08

What is the recommended starting dilution for Western blot for sc-365510: EphB4 (H-10) monoclonal antibody?

Asked by: Trav11
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from an excellent antibody for WBThis antibody recognizes a clear and strong band of predicted M.W. on the western blot (1:500 dilution, 40 ug U251 lysate).
Date published: 2017-08-28
Rated 5 out of 5 by from It's a good antibody.We have also tested this antibody against several cell lines and the antibody works equally well.
Date published: 2017-02-13
Rated 5 out of 5 by from very strong and clear.On the whole, we are satisfied with EphB4 antibody. The analysis results of Western blot were very strong and clear.
Date published: 2017-02-09
Rated 5 out of 5 by from Single strong band in WB in JEGSingle strong band in WB in JEG-3 whole cell lysate. -SCBT QC
Date published: 2015-03-09
Rated 5 out of 5 by from Robust signal in WB in HeLa whole cell lysateRobust signal in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2014-04-01
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EphB4 抗体 (H-10) is rated 5.0 out of 5 by 5.
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