Date published: 2025-9-7

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ENX-1 抗体 (D-8): sc-137255

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说明书
  • ENX-1 抗体 D-8 是小鼠单克隆 IgG2b κ,ENX-1抗体, 在7篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的ENX-1的内部区域内的氨基酸63-102之间
  • ENX-1 抗体 (D-8) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 ENX-1; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 可用于ChIP的TransCruz试剂(sc-137255 X, 200 µg/0.1 ml)
  • 抗ENX-1抗体(D-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ENX-1 (D-8): sc-137255免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是ENX-1 Antibody (D-8) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与ENX-1 Antibody (D-8) 打包提供(请参阅下面的订购信息)。

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関連項目

ENX-1抗体(D-8)是一种IgG2b κ小鼠单克隆ENX-1抗体(也称为Zeste 2增强子抗体、Zeste 2多梳抑制复合体2亚基增强子抗体、ENX-1抗体、KMT6抗体、组蛋白-赖氨酸N-甲基转移酶EZH2抗体、赖氨酸N-甲基转移酶6抗体、Zeste同源增强子2抗体、KMT6A抗体、Zeste(果蝇)同源增强子2抗体、Zeste同源增强子2(果蝇)抗体、EZH2b抗体、ENX1抗体、WVS2抗体、EZH2抗体或EZH1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的ENX-1蛋白。ENX-1抗体(D-8)既可作为非共轭的抗ENX-1抗体形式,也可作为多种共轭形式的抗ENX-1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。在果蝇中,多梳(PcG)基因家族编码染色质蛋白,这些蛋白在胚胎发育中抑制同源异型位点是必需的。PcG蛋白与三胸集团(trxG)协同工作,后者在胚胎发育过程中激活同源框基因表达。ENX-1是果蝇基因增强子zeste的哺乳动物同源物,是一种PcG蛋白,在早期胚胎发育过程中普遍表达,在后期发育过程中仅限于中枢神经系统、外周神经系统和胎儿造血部位。在成人中,ENX-1仅限于特定部位,包括脾脏、睾丸和胎盘。编码人类ENX-1的基因转录出一个包含746个氨基酸的多肽,该多肽含有一个三胸样结构域和一个DNA结合基序。ENX-1与原癌基因产物Vav相互作用,被认为参与正常和恶性造血细胞的增殖。通过改变靶基因的调控,ENX-1也可能导致唐氏综合征的某些表型。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ENX-1 抗体 (D-8) 参考文献:

  1. ENX-1(一种推定的人类多聚酶组基因)参与造血细胞的增殖。  |  Fukuyama, T., et al. 2000. Br J Haematol. 108: 842-7. PMID: 10792293
  2. Polycomb组蛋白Enhancer of Zeste 2:与DNA修复和乳腺癌的关系。  |  Zeidler, M. and Kleer, CG. 2006. J Mol Histol. 37: 219-23. PMID: 16855786
  3. bmi-1 和 mel-18 基因产物定义了一个参与细胞增殖和肿瘤发生的 DNA 结合蛋白新家族。  |  Goebl, MG. 1991. Cell. 66: 623. PMID: 1823128
  4. 子宫上皮完整性需要 Zeste 2 多聚核酸抑制复合体 2 亚基的增强子。  |  Fang, X., et al. 2019. Am J Pathol. 189: 1212-1225. PMID: 30954472
  5. 增强子2多聚体抑制复合物2亚基的多态性增加黑色素瘤皮肤癌的风险。  |  Belpinati, F., et al. 2020. Exp Dermatol. 29: 980-986. PMID: 32748461
  6. 组蛋白甲基转移酶增强子Zeste 2多核苷酸抑制复合物2亚基通过调节小胶质细胞极化加剧抑郁症大鼠的炎症。  |  Huang, X., et al. 2022. Bioengineered. 13: 5509-5524. PMID: 35172677
  7. MicroRNA-34b-5p结合增强子Zeste 2抑制乳脂球-EGF因子8的表达,从而影响肝纤维化。  |  Ma, J., et al. 2022. J Physiol Biochem. 78: 885-895. PMID: 36138295
  8. Vav与ENX-1(一种转录物调节因子,可调控同源框基因的表达)相互作用。  |  Hobert, O., et al. 1996. Mol Cell Biol. 16: 3066-73. PMID: 8649418
  9. 新型小鼠多聚核糖体组基因 Enx-1 的分离与发育表达分析  |  Hobert, O., et al. 1996. Mech Dev. 55: 171-84. PMID: 8861097
  10. 多角体组基因泽斯特增强子的哺乳动物同源物在果蝇异染色质和酿酒葡萄孢端粒中介导基因沉默。  |  Laible, G., et al. 1997. EMBO J. 16: 3219-32. PMID: 9214638
  11. 小鼠多聚核糖体(Pc-G)蛋白 Enx1 和 Enx2 与 Eed 的相互作用:表明存在独立的 Pc-G 复合物。  |  van Lohuizen, M., et al. 1998. Mol Cell Biol. 18: 3572-9. PMID: 9584197
  12. 对哺乳动物多聚酶群蛋白 Enx1/EZH2 和 EED 之间相互作用的表征表明,哺乳动物多聚酶群蛋白复合物之间存在差异。  |  Sewalt, RG., et al. 1998. Mol Cell Biol. 18: 3586-95. PMID: 9584199

订购信息

产品名称产品编号规格价格数量收藏夹

ENX-1 抗体 (D-8)

sc-137255
200 µg/ml
RMB2377.00

ENX-1 (D-8): m-IgG2b BP-HRP 套装

sc-548671
200 µg Ab; 10 µg BP
RMB2662.00

ENX-1 抗体 (D-8) X

sc-137255 X
200 µg/0.1 ml
RMB2377.00

ENX-1 抗体 (D-8) AC

sc-137255 AC
500 µg/ml, 25% agarose
RMB3129.00

ENX-1 抗体 (D-8) HRP

sc-137255 HRP
200 µg/ml
RMB2377.00

ENX-1 抗体 (D-8) FITC

sc-137255 FITC
200 µg/ml
RMB2482.00

ENX-1 抗体 (D-8) PE

sc-137255 PE
200 µg/ml
RMB2580.00

ENX-1 抗体 (D-8) Alexa Fluor® 488

sc-137255 AF488
200 µg/ml
RMB2685.00

ENX-1 抗体 (D-8) Alexa Fluor® 546

sc-137255 AF546
200 µg/ml
RMB2685.00

ENX-1 抗体 (D-8) Alexa Fluor® 594

sc-137255 AF594
200 µg/ml
RMB2685.00

ENX-1 抗体 (D-8) Alexa Fluor® 647

sc-137255 AF647
200 µg/ml
RMB2685.00

ENX-1 抗体 (D-8) Alexa Fluor® 680

sc-137255 AF680
200 µg/ml
RMB2685.00

ENX-1 抗体 (D-8) Alexa Fluor® 790

sc-137255 AF790
200 µg/ml
RMB2685.00

ENX-1 (D-8) 中和勝肽

sc-137255 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-137255 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Not good for western blotWe tested the antibody for western blot using three different human cell lines, tried different dilutions and O/N incubation, didn't got any band. It did not work for me.
Date published: 2021-10-15
Rated 2 out of 5 by from ENX-1 didn't work for MEFs for meI'm sorry to say, but I tried different concentrations and washing conditions it didn't show correct size band in mouse MEFs. This does not rule out the possibility that the expression of EZH2 in MEFs to be low. It may work for another mouse cells but I didn't test.
Date published: 2018-07-09
Rated 3 out of 5 by from Work well!1:200 diluted for wb of rat protein,the band is not quite strong.
Date published: 2018-03-19
Rated 3 out of 5 by from The band is a little weak1:200 diluted for wb of rat heart protein,the band is a little weak
Date published: 2018-02-13
Rated 1 out of 5 by from not useble for Western blot analysisVery disappointing. For Western blot analysis definitely not working. You just ruin your time. I tried different ratios and inkubation times for in the end no detectable signals. Horrible!
Date published: 2018-02-04
Rated 5 out of 5 by from 功能很不错公司免费的小样抗体,最近试了一下,发现这管抗体的特异性非常好,并且没有其他杂带,整张膜非常干净,好评!
Date published: 2017-12-15
Rated 4 out of 5 by from EZH2 in cell linesthis antibody works well for western blot. i got stong band.
Date published: 2017-11-13
Rated 4 out of 5 by from Great for WesternsThe antibody works really well on western blots. I was able to detect endogenous EZH2 in splenic cells.
Date published: 2017-09-02
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ENX-1 抗体 (D-8) is rated 3.6 out of 5 by 15.
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