Date published: 2025-9-7

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Enolase 抗体 (A-5): sc-271384

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说明书
  • Enolase抗体(A-5)是小鼠单克隆IgG1 κ, 在24篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的α Enolase的N-terminus
  • 抗-Enolase 抗体 (A-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 α Enolase, β Enolase and γ Enolase
  • 抗Enolase抗体(A-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Enolase (A-5): sc-271384免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Enolase Antibody (A-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Enolase Antibody (A-5) 打包提供(请参阅下面的订购信息)。

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関連項目

Enolase 抗体(A-5)是一种 IgG1 κ 鼠单克隆 Enolase 抗体,可通过 WB、IP、IF、IHC(P)和 ELISA 检测到小鼠、大鼠和人类的 α-Enolase、β-Enolase 和 γ-Enolase。Enolase 抗体(A-5)既可以是非偶联的抗 Enolase 抗体形式,也可以是多种偶联形式的抗 Enolase 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。烯醇化酶已被鉴定为高度保守的细胞质糖酵解酶,可能参与分化。已鉴定出三种同工酶:α-烯醇化酶、β-烯醇化酶和 γ-烯醇化酶。在大多数组织中检测到 α-烯醇化酶的表达,而 β-烯醇化酶主要在肌肉组织中表达,γ-烯醇化酶仅在神经组织中检测到。这些同工酶以同源二聚体和异源二聚体的形式存在,并在糖酵解途径中将磷酸甘油酸转化为磷酸烯醇丙酮酸中发挥作用。由 433 个氨基酸组成的蛋白质与酵母烯醇化酶具有 67% 的同源性,与大鼠非神经烯醇化酶具有 94% 的同源性。研究还表明,α-烯醇化酶由与编码 τ-晶状体蛋白(一种晶状体结构蛋白)的相同基因编码。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Enolase 抗体 (A-5) 参考文献:

  1. 神经元特异性烯醇化酶的临床生物化学。  |  Kaiser, E., et al. 1989. Clin Chim Acta. 183: 13-31. PMID: 2548772
  2. 烯醇化酶特异性交叉抗体诱导肠道中性粒细胞炎症。  |  Lin, J., et al. 2021. J Leukoc Biol. 109: 633-644. PMID: 32810329
  3. 人α烯醇化酶全长 cDNA 的分子克隆和核苷酸序列。  |  Giallongo, A., et al. 1986. Proc Natl Acad Sci U S A. 83: 6741-5. PMID: 3529090
  4. 淫羊藿素通过上调α-烯醇化酶的表达促进成骨分化。  |  Xie, D., et al. 2023. Biochem Biophys Rep. 34: 101471. PMID: 37125075
  5. 烯醇化酶抑制剂作为抗布鲁氏锥虫的早期先导疗法。  |  Roster, CP., et al. 2023. Pathogens. 12: PMID: 38003754
  6. 突触的形成与禽类听觉和前庭系统神经元特异性烯醇化酶免疫反应的开始有关。  |  Whitehead, MC., et al. 1982. Dev Neurosci. 5: 298-307. PMID: 7140580
  7. 从酵母到哺乳动物,编码烯醇化酶的 DNA 序列非常保守。  |  Verma, M. and Dutta, SK. 1994. Life Sci. 55: 893-9. PMID: 7520111
  8. 成年大鼠大脑神经元中α和γ烯醇化酶基因的共表达。  |  Keller, A., et al. 1994. J Neurosci Res. 38: 493-504. PMID: 7815469
  9. 关于α-和γ-烯醇化酶亚基在培养的大鼠神经细胞和成纤维细胞中分布的比较研究。  |  Deloulme, JC., et al. 1997. Int J Dev Neurosci. 15: 183-94. PMID: 9178037
  10. 大鼠神经胶质细胞中两种神经元标志物--生长缔合作用蛋白 43 和神经元特异性烯醇化酶--的表达。  |  Sensenbrenner, M., et al. 1997. J Mol Med (Berl). 75: 653-63. PMID: 9351704
  11. 烯醇酶的机理:2.0 A 分辨率的不对称二聚体烯醇酶-2-磷酸-D-甘油酸/烯醇酶-磷酸烯醇丙酮酸的晶体结构。  |  Zhang, E., et al. 1997. Biochemistry. 36: 12526-34. PMID: 9376357
  12. Tau-结晶素/α-烯醇化酶:一个基因同时编码一种酶和一种晶状体结构蛋白。  |  Wistow, GJ., et al. 1988. J Cell Biol. 107: 2729-36. PMID: 2462567

订购信息

产品名称产品编号规格价格数量收藏夹

Enolase 抗体 (A-5)

sc-271384
200 µg/ml
RMB2377.00

Enolase (A-5): m-IgGκ BP-HRP 套装

sc-521925
200 µg Ab, 40 µg BP
RMB2662.00

Enolase (A-5): m-IgG1 BP-HRP 套装

sc-533507
200 µg Ab; 20 µg BP
RMB2662.00

Enolase 抗体 (A-5) AC

sc-271384 AC
500 µg/ml, 25% agarose
RMB3129.00

Enolase 抗体 (A-5) HRP

sc-271384 HRP
200 µg/ml
RMB2377.00

Enolase 抗体 (A-5) FITC

sc-271384 FITC
200 µg/ml
RMB2482.00

Enolase 抗体 (A-5) PE

sc-271384 PE
200 µg/ml
RMB2580.00

Enolase 抗体 (A-5) Alexa Fluor® 488

sc-271384 AF488
200 µg/ml
RMB2685.00

Enolase 抗体 (A-5) Alexa Fluor® 546

sc-271384 AF546
200 µg/ml
RMB2685.00

Enolase 抗体 (A-5) Alexa Fluor® 594

sc-271384 AF594
200 µg/ml
RMB2685.00

Enolase 抗体 (A-5) Alexa Fluor® 647

sc-271384 AF647
200 µg/ml
RMB2685.00

Enolase 抗体 (A-5) Alexa Fluor® 680

sc-271384 AF680
200 µg/ml
RMB2685.00

Enolase 抗体 (A-5) Alexa Fluor® 790

sc-271384 AF790
200 µg/ml
RMB2685.00

How can Enolase (A-5): sc-271384 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Germaine
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-271384 HRP), phycoerythrin (sc-271384 PE), fluoroscein (sc-271384 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Strong bindA strong clear bind. It was useful to me, recommended to use!
Date published: 2017-10-20
Rated 4 out of 5 by from Good in WB and IPTested in 32D mFLT3 ITD cells for Western Blot and IP
Date published: 2017-10-19
Rated 5 out of 5 by from Very good antibodyA very strong band at the right size, recommended to use.
Date published: 2017-07-15
Rated 5 out of 5 by from excellent for WBOnly one strong, clear and right size band apears in human cell lysate.
Date published: 2017-04-12
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic staining in formalin-fixed Hep G2 cells. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Robust signal in WB in human transfectedRobust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-16
Rated 5 out of 5 by from Strong signal strength in WB in UStrong signal strength in WB in U-937 and A549 whole cell lysates. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Strong band in WB in HepG2 whole cell lysateStrong band in WB in HepG2 whole cell lysate. -SCBT QC
Date published: 2015-01-13
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Enolase 抗体 (A-5) is rated 4.9 out of 5 by 8.
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