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α Enolase Antibody (L-27) - Western Blotting - Image 34186
a Enolase (L-27): sc-100812. MCF7 全细胞裂解液中 a Enolase 表达的 Western 印迹分析.
a Enolase (L-27): sc-100812. MCF7 全细胞裂解液中 a Enolase 表达的 Western 印迹分析.

α Enolase 抗体 (L-27): sc-100812

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说明书
  • α Enolase抗体(L-27)是小鼠单克隆IgG1 κ, 在32篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组α Enolase
  • 推荐用于 mouse, rat 和 human 来源的α Enolase WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 Enolase (A-5): sc-271384，可查看有标记的 α Enolase 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取α Enolase (L-27): sc-100812的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 α Enolase 抗体 (L-27) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与α Enolase 抗体 (L-27) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Enolase 抗体 包括 α Enolase, β Enolase和γ Enolase

α 烯醇化酶抗体（L-27）是一种小鼠单克隆IgG1κ轻链抗体，可通过多种应用检测小鼠、大鼠和人类样本中的α 烯醇化酶，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）。α 烯醇化酶 单克隆抗体（L-27）以非结合形式提供，适用于多种实验设置。α 烯醇化酶（L-27）抗体可识别在糖酵解途径中发挥关键作用的蛋白质，促进2-磷酸甘油酸向磷酸烯醇丙酮酸的转化，这是能量产生的关键步骤。这种酶的功能对于细胞代谢至关重要，尤其是对能量需求高的组织，如肌肉和大脑。值得注意的是，α-烯醇化酶不仅参与糖酵解，还参与各种细胞过程，包括分化与应激反应。α-烯醇化酶在多种组织中的表达表明其在维持代谢稳态方面的重要性，其表达水平可作为某些病理状况的生物标志物。α-烯醇化酶能够与其他烯醇化酶同工型形成同二聚体和异二聚体，其结构的多功能性进一步增强了其在细胞代谢中的功能意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

α Enolase 抗体 (L-27) 参考文献:

  1. 肌形成中的α-烯醇化酶纤溶酶原受体。  |  Lopez-Alemany, R., et al. 2005. Front Biosci. 10: 30-6. PMID: 15574344
  2. 人α-烯醇化酶基因在口腔上皮细胞和鳞状细胞癌中的差异表达。  |  Ito, S., et al. 2007. Cancer Sci. 98: 499-505. PMID: 17284257
  3. kelch 蛋白 NS1-BP 与 alpha-enolase/MBP-1 相互作用，参与 c-Myc 基因的转录控制。  |  Perconti, G., et al. 2007. Biochim Biophys Acta. 1773: 1774-85. PMID: 17996313
  4. α-烯醇化酶生物学功能的进展。  |  Ji, H., et al. 2016. Anim Nutr. 2: 12-17. PMID: 29767008
  5. 靶向内皮ENO1（α-烯醇化酶）-PI3K-Akt-mTOR轴可缓解缺氧性肺动脉高压。  |  Shi, Y., et al. 2023. Hypertension. 80: 1035-1047. PMID: 37075135
  6. 角蛋白17与α-烯醇化酶共价结合，加剧牛皮癣中角质形成细胞的增殖。  |  Luo, Y., et al. 2023. Int J Biol Sci. 19: 3395-3411. PMID: 37497003
  7. 突触的形成与禽类听觉和前庭系统神经元特异性烯醇化酶免疫反应的开始有关。  |  Whitehead, MC., et al. 1982. Dev Neurosci. 5: 298-307. PMID: 7140580
  8. 从酵母到哺乳动物，编码烯醇化酶的 DNA 序列非常保守。  |  Verma, M. and Dutta, SK. 1994. Life Sci. 55: 893-9. PMID: 7520111
  9. 成年大鼠大脑神经元中α和γ烯醇化酶基因的共表达。  |  Keller, A., et al. 1994. J Neurosci Res. 38: 493-504. PMID: 7815469
  10. 关于α-和γ-烯醇化酶亚基在培养的大鼠神经细胞和成纤维细胞中分布的比较研究。  |  Deloulme, JC., et al. 1997. Int J Dev Neurosci. 15: 183-94. PMID: 9178037
  11. 大鼠神经胶质细胞中两种神经元标志物--生长缔合作用蛋白 43 和神经元特异性烯醇化酶--的表达。  |  Sensenbrenner, M., et al. 1997. J Mol Med (Berl). 75: 653-63. PMID: 9351704
  12. 烯醇酶的机理：2.0 A 分辨率的不对称二聚体烯醇酶-2-磷酸-D-甘油酸/烯醇酶-磷酸烯醇丙酮酸的晶体结构。  |  Zhang, E., et al. 1997. Biochemistry. 36: 12526-34. PMID: 9376357

订购信息

产品名称产品编号规格价格数量收藏夹

α Enolase 抗体 (L-27)

sc-100812
100 µg/ml
RMB2504.00

α Enolase (L-27): m-IgG Fc BP-HRP 套装

sc-537290
100 µg Ab; 10 µg BP
RMB2662.00

α Enolase (L-27): m-IgGκ BP-HRP 套装

sc-534522
100 µg Ab; 40 µg BP
RMB2662.00

sc-100812 抗体一瓶是几微升的呀

Asked by: raziya
感谢您的提问。每个小瓶含有100µg IgG1 kappa轻链，加入1.0 ml含< 0.1%叠氮化钠和0.1%明胶的PBS。 Thank you for your question. Each vial contains 100µg IgG1 Kappa light chain, adding 1.0 mL containing < 0.1% sodium azide and 0.1% gelatin PBS.
Answered by: Zhanglong
Date published: 2022-06-09

Has anyone tried this antibody for FACS?

Asked by: aschw
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2021-12-10

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 10
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with α Enolase (L-27): sc-100812?

Asked by: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-12
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Rated 2 out of 5 by from No hubo reconocimiento de la proteinaLo utilicé para identifircar enolasa en celulas C6/36 pero no hubo reconocimiento de ka proteina
Date published: 2025-01-29
Rated 5 out of 5 by from Perfect signal in WBI tested this antibody in neuronal samples by Western Blotting and it worked perfectly.
Date published: 2018-04-03
Rated 5 out of 5 by from Outstanding !Great western data; also nice IHC and IF staining results
Date published: 2017-01-31
Rated 5 out of 5 by from Popular antibody!This monoclonal alpha Enolase Antibody (L-27) is a very popular choice and has been featured in over a dozen publications. Very well characterized for Western blot.
Date published: 2017-01-30
Rated 3 out of 5 by from No commentNo comment
Date published: 2015-09-23
Rated 5 out of 5 by from Positive signal in WB in MCF7 whole cell lysatePositive signal in WB in MCF7 whole cell lysate. -SCBT QC
Date published: 2015-06-26
Rated 5 out of 5 by from Robust signal in WB in mouse transfectedRobust signal in WB in mouse transfected 293T and A549 whole cell lysates. -SCBT QC
Date published: 2015-05-30
Rated 5 out of 5 by from Superb immunoperoxidase membrane and cytoplasmicSuperb immunoperoxidase membrane and cytoplasmic staining in formalin-fixed, paraffin-embedded human lymphoma tissue. -SCBT QC
Date published: 2015-03-19
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α Enolase 抗体 (L-27) is rated 4.5 out of 5 by 11.
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