Date published: 2025-9-7

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Endomucin 抗体 (V.5C7): sc-53941

4.3(3)
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说明书
  • Endomucin 抗体 V.5C7 是大鼠单克隆 IgG1, 在25篇文献中引用,提供 200 µg/ml
  • 针对bEND.3 mouse来源内皮瘤
  • Endomucin 抗体 (V.5C7) 推荐用于WB, IP, IF 和 FCM,检测mouse 来源的 Endomucin; 不建议用于检测human Endomucin
  • 抗Endomucin抗体(V.5C7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Endomucin (V.5C7): sc-53941免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Endomucin 抗体 (V.5C7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Endomucin 抗体(V.5C7)是一种大鼠单克隆 IgG1 抗体,可通过免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和流式细胞术(FCM)检测小鼠来源的 Endomucin。抗内切蛋白抗体(V.5C7)有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 共轭物。内切黏蛋白又称 EMCN 或 Mucin-14,是一种 261 个氨基酸的蛋白质,具有跨膜序列和许多糖基化位点,由于丝氨酸和苏氨酸残基上存在 O 键连接的聚糖,这些位点促成了其扩展结构。这种蛋白质主要在心脏、肾脏和肺部等血管组织中表达,以两种形式存在:一种是未经加工的前体肽,另一种是经过加工的变体,即内切蛋白 2(由 241 个氨基酸组成)。值得注意的是,小鼠 Endomucin 是一种内皮抗原,存在于静脉内皮和毛细血管中,但在动脉内皮中却不存在。在内皮细胞增殖过程中,以及在肿瘤调节介质或特定血管生成因子(如碱性成纤维细胞生长因子和肿瘤坏死因子-α)的作用下,Endomucin 的表达会上调。重要的是,内切蛋白的过表达已被证明可抑制造血细胞的粘附和聚集,这表明它在造血早期阶段促进这些细胞从内皮脱离的潜在作用,而这对血细胞的正常发育和功能至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Endomucin 抗体 (V.5C7)

sc-53941
200 µg/ml
RMB2377.00

Endomucin 抗体 (V.5C7) AC

sc-53941 AC
500 µg/ml, 25% agarose
RMB3129.00

Endomucin 抗体 (V.5C7) HRP

sc-53941 HRP
200 µg/ml
RMB2377.00

Endomucin 抗体 (V.5C7) FITC

sc-53941 FITC
200 µg/ml
RMB2482.00

Endomucin 抗体 (V.5C7) PE

sc-53941 PE
200 µg/ml
RMB2580.00

Endomucin 抗体 (V.5C7) Alexa Fluor® 488

sc-53941 AF488
200 µg/ml
RMB2685.00

Endomucin 抗体 (V.5C7) Alexa Fluor® 546

sc-53941 AF546
200 µg/ml
RMB2685.00

Endomucin 抗体 (V.5C7) Alexa Fluor® 594

sc-53941 AF594
200 µg/ml
RMB2685.00

Endomucin 抗体 (V.5C7) Alexa Fluor® 647

sc-53941 AF647
200 µg/ml
RMB2685.00

Endomucin 抗体 (V.5C7) Alexa Fluor® 680

sc-53941 AF680
200 µg/ml
RMB2685.00

Endomucin 抗体 (V.5C7) Alexa Fluor® 790

sc-53941 AF790
200 µg/ml
RMB2685.00

Has this antibody been verified for Immunofluorescence? If so, which ones are the references or an example of the stain?

Asked by: liliana10
Thank you for your question. You can find IF data generated with the Endomucin Antibody (V.5C7): sc-53941 in these publication: PMID 25992544 PMID 29773646 PMID 28137359
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

Can you recommend a positive control to use with Endomucin (V.5C7): sc-53941 monoclonal antibody for Western blot?

Asked by: jenni
We recommend mouse kidney (sc-2255) or mouse heart (sc-2254) tissue extracts to use as positive controls as we have Western blot data showing specific detection of Endomucin in these cell lines.
Answered by: Technical Support 8
Date published: 2017-01-17
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Rated 5 out of 5 by from Very strong bandsEndomucin (V.5C7) gives a very strong band in mouse heart extract: sc-2254 at expected molecular weight.
Date published: 2017-01-17
Rated 4 out of 5 by from Robust bands seen in WB using mouse lungRobust bands seen in WB using mouse lung, mouse pancreas and mouse thymus tissue extracts. -SCBT QC
Date published: 2015-06-07
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2013-11-14
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Endomucin 抗体 (V.5C7) is rated 4.3 out of 5 by 3.
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