Date published: 2025-9-7
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Endomucin Antibody (V.7C7) - Immunohistochemistry - Image 361442
内切蛋白抗体 (V. 7C7): sc-65495. 对福尔马林固定, 石蜡包埋的小鼠肾组织进行免疫过氧化物酶染色, 显示肾小球和内皮细胞的细胞膜和细胞质染色.
内切蛋白抗体 (V. 7C7): sc-65495. 对福尔马林固定, 石蜡包埋的小鼠肾组织进行免疫过氧化物酶染色, 显示肾小球和内皮细胞的细胞膜和细胞质染色.

Endomucin 抗体 (V.7C7): sc-65495

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寫評論提問

说明书
  • Endomucin 抗体 V.7C7 是大鼠单克隆 IgG2a，Endomucin抗体, 在426篇文献中引用，规格为200 µg/ml
  • 针对bEND.3 mouse来源内皮瘤
  • Endomucin 抗体 (V.7C7) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse 和rat 来源的 Endomucin; 不建议用于检测human Endomucin
  • 抗Endomucin抗体（V.7C7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Endomucin (V.7C7): sc-65495的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Endomucin 抗体 (V.7C7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Endomucin 抗体（V.7C7）是一种大鼠单克隆 IgG2a 抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和流式细胞术（FCM）检测小鼠和大鼠来源的 Endomucin 蛋白。抗内切酶素抗体（V.7C7）有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 共轭物。内切黏蛋白又称 EMCN 或 Mucin-14，是一种 261 个氨基酸的蛋白质，具有跨膜序列和许多糖基化位点，这对其功能至关重要。丝氨酸和苏氨酸残基上的 O 型糖链迫使内切粘蛋白形成扩展构象，从而增强了与其他分子的相互作用。Endomucin 主要在心脏、肾脏和肺部等血管组织中表达，以两种形式存在：一种是未经加工的前体肽，另一种是经过加工的变体，称为 Endomucin 2，由 241 个氨基酸组成。在小鼠体内，Endomucin 是一种内皮抗原，存在于静脉内皮和毛细血管中，但在动脉内皮中却不存在。值得注意的是，在内皮细胞增殖过程中，以及在肿瘤调节介质或血管生成因子（如碱性成纤维细胞生长因子和肿瘤坏死因子-α）的作用下，Endomucin 的表达会上调。此外，内切酶素的过表达已被证明可抑制造血细胞的粘附和聚集，这表明它在造血早期阶段促进这些细胞从内皮脱离的潜在作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Endomucin 抗体 (V.7C7) 参考文献:

  1. 鉴定具有抗黏合剂活性的膜结合 O-sialoglycoproteins-1和-2。  |  Kinoshita, M., et al. 2001. FEBS Lett. 499: 121-6. PMID: 11418125
  2. 内黏蛋白在胚胎背主动脉中表达，能够抑制细胞黏附。  |  Ueno, M., et al. 2001. Biochem Biophys Res Commun. 287: 501-6. PMID: 11554756
  3. 人类内黏蛋白是一种内皮标志物。  |  Liu, C., et al. 2001. Biochem Biophys Res Commun. 288: 129-36. PMID: 11594763
  4. T 淋巴细胞糖蛋白白细胞介素的尺寸以及可被糖基化修饰的线性蛋白质表位的鉴定。  |  Cyster, JG., et al. 1991. EMBO J. 10: 893-902. PMID: 1706994
  5. 表观遗传学上协调的GATA2结合对于内皮细胞特异性内皮素表达是必需的。  |  Kanki, Y., et al. 2011. EMBO J. 30: 2582-95. PMID: 21666600
  6. α1,3-fucosyltransferase FUT7通过内皮素的fucosylation调节IL-1β诱导的单核细胞-内皮细胞粘附。  |  Zhang, J., et al. 2018. Life Sci. 192: 231-237. PMID: 29138114
  7. 内皮素通过调节VEGFR2信号传导抑制VEGF诱导的内皮细胞迁移、生长和形态发生。  |  Park-Windhol, C., et al. 2017. Sci Rep. 7: 17138. PMID: 29215001
  8. 人类成体造血干细胞可通过内皮素表达与祖细胞造血干细胞区分开来。  |  Reckzeh, K., et al. 2018. Blood Adv. 2: 1628-1632. PMID: 29986855
  9. 血管内皮生长因子诱导的血管内皮生长因子受体 2（VEGFR2）活性所必需的内黏蛋白胞外结构域元素。  |  Hu, Z., et al. 2020. Cells. 9: PMID: 32517158
  10. 一种新型内皮特异性 sialomucin 的生化特征和分子克隆。  |  Morgan, SM., et al. 1999. Blood. 93: 165-75. PMID: 9864158

订购信息

产品名称产品编号规格价格数量收藏夹

Endomucin 抗体 (V.7C7)

sc-65495
200 µg/ml
RMB2377.00

Endomucin 抗体 (V.7C7) AC

sc-65495 AC
500 µg/ml, 25% agarose
RMB3129.00

Endomucin 抗体 (V.7C7) HRP

sc-65495 HRP
200 µg/ml
RMB2377.00

Endomucin 抗体 (V.7C7) FITC

sc-65495 FITC
200 µg/ml
RMB2482.00

Endomucin 抗体 (V.7C7) PE

sc-65495 PE
200 µg/ml
RMB2580.00

Endomucin 抗体 (V.7C7) Alexa Fluor® 488

sc-65495 AF488
200 µg/ml
RMB2685.00

Endomucin 抗体 (V.7C7) Alexa Fluor® 546

sc-65495 AF546
200 µg/ml
RMB2685.00

Endomucin 抗体 (V.7C7) Alexa Fluor® 594

sc-65495 AF594
200 µg/ml
RMB2685.00

Endomucin 抗体 (V.7C7) Alexa Fluor® 647

sc-65495 AF647
200 µg/ml
RMB2685.00

Endomucin 抗体 (V.7C7) Alexa Fluor® 680

sc-65495 AF680
200 µg/ml
RMB2685.00

Endomucin 抗体 (V.7C7) Alexa Fluor® 790

sc-65495 AF790
200 µg/ml
RMB2685.00

What is the light chain isotype of the Endomucin (V.7C7) antibody? kappa or lambda?

Asked by: gokujianghu
Thank you for your question. kappa light chain
Answered by: BlakeJ
Date published: 2023-08-21

请问这是大鼠来源的抗体，还是小鼠来源的抗体？

Asked by: name
感谢您的提问。 Endomucin 抗体 (V.7C7): sc-65495是大鼠来源的抗体。 Thank you for your question. Endomucin (V.7C7) is a rat monoclonal antibody.
Answered by: Ling Chen
Date published: 2024-04-26

I used this AB but it showed same data with negative control, I would like to do double IF of endomucin and CD31 on rat mandible. If I change to endomucin conjugated with Alexa Fluor, is it possible to get better result? sale service to me to change AB.

Asked by: TP_N
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2024-04-26

I accidentally froze this antibody for around about an hour. Should I worry about its performance ?

Asked by: Joeee
Thank you for your question. As this antibody is supplied in a buffer containing gelatin, it will cause the antibody to precipitate when it is frozen. There should not be too much of an impact on the antibody's viability from freezing it once.
Answered by: Tech Support Europe
Date published: 2021-04-14

Hi scbt team, For Endomucin antibody (V.7C7): sc-65495, Can you tell me the price of 1mg？ If I order 1mg, how long can I get it?

Asked by: garden
Thank you for your question. Please contact our Special Orders Department to inquire about availability and to receive a quote for a bulk quantity of this antibody, sc-65495. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Tech Service
Date published: 2019-11-12

What is the difference with clone V.7C7.1? This product (clone V.7C7) is a monoclonal antibody for mouse tissue. I also see the monoclonal antibody of clone V.7C71 that is being sold as an antibody. That one is mentioned to work on human tissue as well.

Asked by: Merl
Thanks for your question. The V.7C7 refers to a specific hybridoma clone used to produce the monoclonal antibody. Please contact technical service for further support.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Will the Endomucin (V.7C7) antibody detect protein in samples of rat origin?

Asked by: Anonymous
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 6
Date published: 2023-10-05

How can this rat monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in rat?

Asked by: Anonymous
We recommend using a directly conjugated version of Endomucin Antibody (V.7C7): sc-65495. This rat monoclonal antibody is available conjugated to HRP (sc-65495 HRP), phycoerythrin (sc-65495 PE), fluoroscein (sc-65495 FITC), Alexa Fluor® 488 (sc-65495 AF488), and Alexa Fluor® 647 (sc-65495 AF647).
Answered by: Tech Service 6
Date published: 2016-12-15
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Rated 5 out of 5 by from Great features.I bought this Endomucin antibody 2 weeks ago for IHC staining. I got the perfect image already.
Date published: 2025-02-15
Rated 5 out of 5 by from Great vascular labeling in bone marrow sections!At first I thought successful endomucin labeling in paraffin sections was a fever dream…until I tried this antibody! Worked like charm~ Used on bone marrow FFPE sections at 1:250
Date published: 2023-12-05
Rated 5 out of 5 by from Very nice sinusoid stainings for bone marrowWorks very good. Used it for Bone Marrow sections and immunofluorescence stainings. Used it for picture related 1:200, but could certainly be diluted more.
Date published: 2019-10-13
Rated 5 out of 5 by from 小鼠血管免疫荧光染色效果很好我一个月前购买此产品，用于小鼠股骨石蜡切片，采用血免疫荧光染色标记血管内皮细胞，使用后染色效果很好。
Date published: 2019-09-18
Rated 4 out of 5 by from Great on cryostat brain sectionsUsed this on PFA fixed brain tissue. Worked well with little background but required cryostat sections, citrate retrieval and TSA signal amplification
Date published: 2018-02-22
Rated 5 out of 5 by from good qualityThis antiobody been used for detect HepG2 cell and MCF-7 cell, also our labmates used it detect other tumor cells by WB.We never meet problems use it,strong band at 80kda.It's very stable and good quality.
Date published: 2017-09-16
Rated 5 out of 5 by from high sensitivitySpecific and sensivity is high for WB, I got the target band at 80 kda.
Date published: 2017-09-05
Rated 5 out of 5 by from Excellent for IF and IHCThis antibody is excellent for both IF and IHC of mouse tissues as both frozen and paraffin sections. For IF of frozen sections, 1:500, +4°C, overnight. For IHC of paraffin sections, 1:500, +4°C, overnight, citrate buffer for antigen retrieval. In the attached image, endomucin staining in green.
Date published: 2017-08-02
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Endomucin 抗体 (V.7C7) is rated 4.8 out of 5 by 15.
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