Date published: 2025-9-6

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eIF4B 抗体 (D-4): sc-376062

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说明书
  • eIF4B 抗体 D-4 是小鼠单克隆 IgG1 κ,eIF4B抗体, 在12篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的eIF4B的C-terminus的氨基酸581-602之间
  • eIF4B 抗体 (D-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 eIF4B
  • 抗eIF4B抗体(D-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取eIF4B (D-4): sc-376062免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是eIF4B Antibody (D-4) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与eIF4B Antibody (D-4) 打包提供(请参阅下面的订购信息)。

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関連項目

eIF4B抗体(D-4)是一种IgG1 κ小鼠单克隆eIF4B抗体(也称为EIF4EB抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的eIF4B蛋白。eIF4B抗体(D-4)既可以非偶联的抗eIF4B抗体形式提供,也可以多种偶联形式的抗eIF4B抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在真核细胞中,蛋白质合成的启动受到蛋白质起始因子和RNA分子之间相互作用的调控。这些相互作用在一定程度上是由参与蛋白质合成早期启动的真核起始因子4家族(eIF4)蛋白所促进的。eIF4B(真核翻译起始因子4B)是一种含有1个RNA识别基序的611个氨基酸的蛋白质,属于eIF4蛋白家族。eIF4B对于正确的mRNA/核糖体结合是必需的,它与其他eIF4蛋白(如eIF4A)结合,并促进特定真核起始因子的ATP依赖性解旋活性。编码eIF4B的基因映射到人类12号染色体上,该染色体编码1100多个基因,约占人类基因组的4.5%。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

eIF4B 抗体 (D-4) 参考文献:

  1. Caspase 和病毒蛋白酶介导的裂解破坏了哺乳动物蛋白质合成真核启动因子 4B 与聚(A)结合蛋白的相互作用。  |  Bushell, M., et al. 2001. J Biol Chem. 276: 23922-8. PMID: 11274152
  2. 真核生物翻译引发剂 4B 的 RNA 识别基序的溶液结构和 RNA 相互作用。  |  Fleming, K., et al. 2003. Biochemistry. 42: 8966-75. PMID: 12885229
  3. eIF4AI 及其附属因子 eIF4B 和 eIF4H 之间的相互作用。  |  Rozovsky, N., et al. 2008. RNA. 14: 2136-48. PMID: 18719248
  4. 真核生物引发剂 4B cDNA 的克隆与表达:序列测定确定了一个共同的 RNA 识别基序。  |  Milburn, SC., et al. 1990. EMBO J. 9: 2783-90. PMID: 2390971
  5. 毒蕈碱乙酰胆碱受体介导 SNU-407 结肠癌细胞中的 eIF4B 磷酸化。  |  Liu, Z. and Cho, NJ. 2016. Biochem Biophys Res Commun. 480: 450-454. PMID: 27773818
  6. eIF4B 可增强 ATF4 的表达,有助于细胞适应 BRAF 突变的 A375 黑色素瘤中天冬酰胺的限制。  |  Iwao, Y., et al. 2021. Biochem Biophys Res Commun. 573: 93-99. PMID: 34403810
  7. 缺乏 eIF4B 会增加小鼠死亡率并损害抗病毒免疫力  |  Chen, B., et al. 2021. Front Immunol. 12: 723885. PMID: 34566982
  8. PARK2调节eIF4B驱动的淋巴瘤发生。  |  Kapadia, BB., et al. 2022. Mol Cancer Res.. PMID: 35105670
  9. 转化引发剂和核糖体与 HeLa 细胞的细胞骨架框架部分的缔合作用。  |  Howe, JG. and Hershey, JW. 1984. Cell. 37: 85-93. PMID: 6722878
  10. 翻译起始因子 eIF-4B 包含一个与核糖核蛋白共识序列不同且独立的 RNA 结合区。  |  Méthot, N., et al. 1994. Mol Cell Biol. 14: 2307-16. PMID: 8139536
  11. 启动因子 eIF-4B 的两个结构域参与了与 RNA 的结合。  |  Naranda, T., et al. 1994. J Biol Chem. 269: 14465-72. PMID: 8182051
  12. 富含天冬氨酸, 精氨酸, 酪氨酸和甘氨酸(DRYG)的区域介导真核启动因子 4B (eIF4B)的自我结合以及与 eIF3 的相互作用。  |  Méthot, N., et al. 1996. Mol Cell Biol. 16: 5328-34. PMID: 8816444

订购信息

产品名称产品编号规格价格数量收藏夹

eIF4B 抗体 (D-4)

sc-376062
200 µg/ml
RMB2377.00

eIF4B (D-4): m-IgG Fc BP-HRP 套装

sc-529813
200 µg Ab; 10 µg BP
RMB2662.00

eIF4B (D-4): m-IgGκ BP-HRP 套装

sc-522835
200 µg Ab, 40 µg BP
RMB2662.00

eIF4B (D-4): m-IgG1 BP-HRP 套装

sc-543703
200 µg Ab; 20 µg BP
RMB2662.00

eIF4B 抗体 (D-4) AC

sc-376062 AC
500 µg/ml, 25% agarose
RMB3129.00

eIF4B 抗体 (D-4) HRP

sc-376062 HRP
200 µg/ml
RMB2377.00

eIF4B 抗体 (D-4) FITC

sc-376062 FITC
200 µg/ml
RMB2482.00

eIF4B 抗体 (D-4) PE

sc-376062 PE
200 µg/ml
RMB2580.00

eIF4B 抗体 (D-4) Alexa Fluor® 488

sc-376062 AF488
200 µg/ml
RMB2685.00

eIF4B 抗体 (D-4) Alexa Fluor® 546

sc-376062 AF546
200 µg/ml
RMB2685.00

eIF4B 抗体 (D-4) Alexa Fluor® 594

sc-376062 AF594
200 µg/ml
RMB2685.00

eIF4B 抗体 (D-4) Alexa Fluor® 647

sc-376062 AF647
200 µg/ml
RMB2685.00

eIF4B 抗体 (D-4) Alexa Fluor® 680

sc-376062 AF680
200 µg/ml
RMB2685.00

eIF4B 抗体 (D-4) Alexa Fluor® 790

sc-376062 AF790
200 µg/ml
RMB2685.00

eIF4B (D-4) 中和勝肽

sc-376062 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-376062 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from eIF4B antibodyThe eIF4B antibody (sc-376062) was working very well in our Western blotting.
Date published: 2021-11-01
Rated 5 out of 5 by from ExcellentI used with 1:1000 dilution in human breast cancer cells. Really strong bands.
Date published: 2018-10-10
Rated 5 out of 5 by from Great antibody!This antibody produced greatly strong and specific signal on WB without background.
Date published: 2017-05-31
Rated 5 out of 5 by from WorthAs shown by reference, the initiation of protein synthesis in eukaryotic cells is regulated by the action of the protein initiation factor. Although we ultimately failed, but we think the purchase is worth it
Date published: 2017-02-09
Rated 5 out of 5 by from Excellent!This antibody is recommended for western blot analysis of eIF4B expression in HUV-EC-C and Ramos whole cell lysates.
Date published: 2017-01-26
Rated 5 out of 5 by from Highly potent and fairly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~80 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for a medium-strong ~52 kDa protein.
Date published: 2016-11-10
Rated 5 out of 5 by from IHC(P): good data seen with human pancreas tissueIHC(P): good data seen with human pancreas tissue, cytoplasmic staining of Islets of Langerhans and glandular cells. -SCBT QC
Date published: 2015-01-22
Rated 5 out of 5 by from Great for Western BlotGreat for Western Blot. Good results with MCF7, HeLa, Ramos, A-431 and NIH/3T3 cells. -SCBT QC
Date published: 2013-01-16
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eIF4B 抗体 (D-4) is rated 5.0 out of 5 by 8.
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