Date published: 2025-9-7

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eIF2α 抗体 (G-12): sc-133227

4.8(5)
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说明书
  • eIF2α抗体(G-12)是小鼠单克隆IgG2b κ, 在23篇文献中引用,规格为200 µg/ml
  • 免疫human物种的代表全长eIF2α的氨基酸1-315
  • 推荐用于 mouse, rat 和 human 来源的eIF2α WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 eIF2α (D-3): sc-133132,可查看有标记的 eIF2α 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG2b BP-HRP 是eIF2α 抗体 (G-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与eIF2α 抗体 (G-12) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

在真核细胞中,蛋白质合成的启动受蛋白质起始因子和RNA分子之间相互作用的调控。真核起始复合物由三个亚基组成,分别称为eIF2α、eIF2β和eIF2γ(即真核翻译起始因子2 α、β和γ),它们在蛋白质合成的早期阶段协同工作,与GTP和tRNA形成三元复合物。eIF2α,也称为EIF2S1或EIF2,是真核起始复合物的315个氨基酸亚基,其功能是以GTP依赖的方式将tRNA结合到40S核糖体亚基上,从而启动翻译。此外,eIF2α的磷酸化状态控制着tRNA的翻译速率。当eIF2α不被磷酸化时,翻译以正常速率进行。然而,当eIF2α被几种激酶之一磷酸化时,eIF2α会稳定下来,从而阻止GDP/GTP交换反应并减缓翻译。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

eIF2α 抗体 (G-12)

sc-133227
200 µg/ml
$316.00

eIF2α (G-12): m-IgG2b BP-HRP 套装

sc-548488
200 µg Ab; 10 µg BP
$354.00

I am using eIF2α (G-12): sc-133227 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great productI have just tried this antibody and it gave me highly specific bands at the size that is mentioned and expected for eIF2alpha. I tried it at a dilution of 1:100 in 5% NFDM prepared in TBST.
Date published: 2020-08-07
Rated 4 out of 5 by from GreatA good antibody to detect human eIF2a in 293T cells
Date published: 2018-11-19
Rated 5 out of 5 by from can be usedused in the wb, meth-induced apoptosis, the band is clear.
Date published: 2017-03-07
Rated 5 out of 5 by from Strong cytoplasmic staining of glandularStrong cytoplasmic staining of glandular cells in IHC(P) with human rectum tissue -SCBT QC
Date published: 2015-04-10
Rated 5 out of 5 by from Robust bands with 293TRobust bands with 293T, HeLa, K-562, NIH/3T3 and KNRK whole cell lysates in WB -SCBT QC
Date published: 2014-10-06
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eIF2α 抗体 (G-12) is rated 4.8 out of 5 by 5.
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