Date published: 2025-9-7
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eIF2α Antibody (D-3) - Western Blotting - Image 352797
eIF2α 抗体 (D-3): sc-133132. eIF2α 在 HeLa (A), Jurkat (B), PC-12 (C), A-431 (D) 和 NIH/3T3 (E) 全细胞裂解液以及小鼠胎盘组织提取物 (F) 中表达的 Western 印迹分析.
eIF2α 抗体 (D-3): sc-133132. eIF2α 在 HeLa (A), Jurkat (B), PC-12 (C), A-431 (D) 和 NIH/3T3 (E) 全细胞裂解液以及小鼠胎盘组织提取物 (F) 中表达的 Western 印迹分析.

eIF2α 抗体 (D-3): sc-133132

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说明书
  • eIF2 alpha 抗体 D-3 是小鼠单克隆 IgG1 κ，eIF2α抗体, 在148篇文献中引用，规格为200 µg/ml
  • 针对酸1-315，代表human来源的全长eIF2α
  • eIF2 alpha 抗体 (D-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 eIF2α
  • 抗eIF2 alpha抗体（D-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取eIF2α (D-3): sc-133132的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 eIF2α 抗体 (D-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与eIF2α 抗体 (D-3) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外eIF抗体，包括eIF1, eIF1AY, eIF2 α, eIF2B α, eIF2B β, eIF2B δ, eIF2B ε, eIF2 β, eIF2B γ和eIF2C

eIF2α 抗体（D-3）是一种 IgG1 κ 小鼠单克隆 eIF2 alpha 抗体（也称为 EIF2A 抗体、eIF2 alpha 抗体或 EIF2S1 抗体），可通过 WB、IP、IF、IHC(P) 和 ELISA 检测小鼠、大鼠和人类的 eIF2 alpha 蛋白。eIF2α 抗体（D-3）既可以是非偶联的抗 eIF2 alpha 抗体形式，也可以是多种偶联形式的抗 eIF2 alpha 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。真核细胞中蛋白质合成的启动受蛋白质起始因子和 RNA 分子之间相互作用的调控。真核起始复合物由三个亚基组成，分别称为 eIF2α、eIF2β 和 eIF2γ（即真核翻译起始因子 2 α、β 和 γ），它们在蛋白质合成的早期阶段协同工作，与 GTP 和 tRNA 形成三元复合物。eIF2α，也称为 EIF2S1 或 EIF2，是真核起始复合物的 315 个氨基酸亚基，其功能是以 GTP 依赖的方式将 tRNA 结合到 40S 核糖体亚基上，从而启动翻译。此外，eIF2α 的磷酸化状态控制着 tRNA 的翻译速率。当 eIF2α 不被磷酸化时，翻译以正常速率进行。然而，当 eIF2α 被几种激酶之一磷酸化时，eIF2α 会稳定下来，从而阻止 GDP/GTP 交换反应并减缓翻译。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

eIF2α 抗体 (D-3) 参考文献:

  1. eIF2alpha 去磷酸化的选择性抑制剂可保护细胞免受 ER 应激。  |  Boyce, M., et al. 2005. Science. 307: 935-9. PMID: 15705855
  2. 真核生物翻译引发过程中，起始位点选择所控制的步骤是从 eIF2 中释放 Pi，而不是 GTP 的水解。  |  Algire, MA., et al. 2005. Mol Cell. 20: 251-62. PMID: 16246727
  3. 人类翻译引发剂 2（eIF2）亚基的表达和纯化：eIF2 alpha 和 beta 的磷酸化。  |  Suragani, RN., et al. 2006. Protein Expr Purif. 47: 225-33. PMID: 16289913
  4. 核酸引发因子 2 的磷酸化状态会改变特定 mRNA 的翻译效率。  |  Kaufman, RJ., et al. 1989. Mol Cell Biol. 9: 946-58. PMID: 2657393
  5. 真核启动因子 eIF-2 和 GTP 在蛋白质合成引发剂过程中的结合与释放。  |  Trachsel, H. and Staehelin, T. 1978. Proc Natl Acad Sci U S A. 75: 204-8. PMID: 272635
  6. 翻译起始因子 eIF-2 α-亚基互补 DNA 的克隆和测序。蛋白质及其信使 RNA 的特征。  |  Ernst, H., et al. 1987. J Biol Chem. 262: 1206-12. PMID: 2948954
  7. 翻译起始因子 eIF-2 beta 亚基的结构。  |  Pathak, VK., et al. 1988. Cell. 54: 633-9. PMID: 3044606
  8. 真核生物引发剂 eIF-2 与 GTP 和 Met-tRNA 形成三元复合物的活性。  |  Benne, R., et al. 1979. J Biol Chem. 254: 3201-5. PMID: 429345
  9. 翻译引发剂 eIF-2。克隆和表达编码γ-亚基的人类cDNA。  |  Gaspar, NJ., et al. 1994. J Biol Chem. 269: 3415-22. PMID: 8106381

订购信息

产品名称产品编号规格价格数量收藏夹

eIF2α 抗体 (D-3)

sc-133132
200 µg/ml
RMB2377.00

eIF2α (D-3): m-IgGκ BP-HRP 套装

sc-521244
200 µg Ab, 40 µg BP
RMB2662.00

eIF2α (D-3): m-IgG1 BP-HRP 套装

sc-533484
200 µg Ab; 20 µg BP
RMB2662.00

eIF2α 抗体 (D-3) AC

sc-133132 AC
500 µg/ml, 25% agarose
RMB3129.00

eIF2α 抗体 (D-3) HRP

sc-133132 HRP
200 µg/ml
RMB2377.00

eIF2α 抗体 (D-3) FITC

sc-133132 FITC
200 µg/ml
RMB2482.00

eIF2α 抗体 (D-3) PE

sc-133132 PE
200 µg/ml
RMB2580.00

eIF2α 抗体 (D-3) Alexa Fluor® 488

sc-133132 AF488
200 µg/ml
RMB2685.00

eIF2α 抗体 (D-3) Alexa Fluor® 546

sc-133132 AF546
200 µg/ml
RMB2685.00

eIF2α 抗体 (D-3) Alexa Fluor® 594

sc-133132 AF594
200 µg/ml
RMB2685.00

eIF2α 抗体 (D-3) Alexa Fluor® 647

sc-133132 AF647
200 µg/ml
RMB2685.00

eIF2α 抗体 (D-3) Alexa Fluor® 680

sc-133132 AF680
200 µg/ml
RMB2685.00

eIF2α 抗体 (D-3) Alexa Fluor® 790

sc-133132 AF790
200 µg/ml
RMB2685.00

Does this antibody recognize eIF2alpha regardless of its phosphorylation? 이 안티바디는 phosphorylated form과 unphosphorylated form 둘다 잡나요?

Asked by: abcdef25
Thank you for your question. eIF2α Antikörper (D-3): sc-133132 was raised against acids 1-315 representing full length eIF2α of human origin and should detect both phosphorylated and unphosphorylated forms of eIF2α.
Answered by: Tech Support Europe
Date published: 2020-07-16

Hi, Would this antibody work in yeast? or could you give is its region of target in eif2alpha? additionally, is SC-11386 antibody available to purchase?

Asked by: thariz
Thanks for your questions. eIF2α Antibody (D-3): sc-133132 is raised against acids 1-315 representing full length eIF2α of human origin.This antibody has not yet been tested in yeast and so that is not a recommended species. eIF2α (FL-315) :sc-11386 has been discontinued and replaced by eIF2α (D-3): sc-133132.
Answered by: Technical Support 9
Date published: 2020-06-12

Could you please provide the datasheet of the discontinued antibody sc-101670 for p-eIF2alpha? Thank you Ana Sayuri Yamagata

Asked by: Ana Sayuri Yamagata
Thank you for your question. Here is a link to the datasheet: https://datasheets.scbt.com/sc-101670.pdf
Answered by: Tech Service
Date published: 2020-05-29

CHOP is about 21 kDa, why is the band in your example above 34K?

Asked by: Droso
Thank you for your question. This antibody, eIF2α (D-3): sc-133132, recognizes a protein designated eIF2α, also known as EIF2S1 or EIF2. The predicted molecular weight of eIF2α is ~36 kDa.
Answered by: Technical Support
Date published: 2019-01-26

I am an interested in using sc-133132 as a part of a double stain. Do you have any suggestions about how to do this with only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using a directly conjugated primary antibody. This product, sc-133132, is available directly conjugated to AF488, AF647, and FITC.
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 4 out of 5 by from Good work!This antibody gave a strong and clean signal when we used it at a 1:100 diluition with 80μg of protein samples.
Date published: 2023-01-20
Rated 5 out of 5 by from Good resultsThis antibody works well in western blot of human whole cell extracts.
Date published: 2021-05-31
Rated 3 out of 5 by from Good signal but with some backgroundThe bands were intense and specific but there was a bit background. Maybe blocking for 2 hours instead of 1 would improve it.
Date published: 2021-03-17
Rated 1 out of 5 by from medioit's recognized a 2 band at 37 and 25 KDA on mouse brain homogenates
Date published: 2020-01-28
Rated 5 out of 5 by from western blotting on mouse brain tissueWe tested this antibody in the mouse brain tissue. It works great for western blotting on mouse brain tissue
Date published: 2019-11-04
Rated 1 out of 5 by from did not work for IFNo signal in immunofluorescent staining. Used 1:50, overnight (4C), on mixed primary mouse ventral horn cultures, under basal and stressed (sodium arsenite) conditions. cells were fixed with 4% PFA. Disappointing.
Date published: 2017-11-22
Rated 5 out of 5 by from effect and a clearAntibody quality was good and WB diluted 1:1000 was used in mouse placenta tissue, showing strong bands.
Date published: 2017-10-26
Rated 4 out of 5 by from Good antibody antibodyI used this antibody with a dilution of 1:300 and got a clear band
Date published: 2017-10-23
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eIF2α 抗体 (D-3) is rated 4.6 out of 5 by 36.
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