Date published: 2025-9-7
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EGR1 Antibody (B-6) - Western Blotting - Image 391873
Egr-1 抗体 (B-6): sc-515830. NIH/3T3 全细胞裂解液 (A), A-431 (B), HeLa (C), COLO 320DM (D) 和 HL-60 (E) 核提取物中 Egr-1 表达的 Western 印迹分析.
Egr-1 抗体 (B-6): sc-515830. NIH/3T3 全细胞裂解液 (A), A-431 (B), HeLa (C), COLO 320DM (D) 和 HL-60 (E) 核提取物中 Egr-1 表达的 Western 印迹分析.

Egr-1 抗体 (B-6): sc-515830

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说明书
  • EGR1 抗体 B-6 是小鼠单克隆 IgG1 κ，EGR1抗体, 在23篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的的C-terminus的氨基酸524-543之间
  • EGR1 抗体 (B-6) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Egr-1 p82
  • 抗EGR1抗体（B-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-515830 X, 200 µg/0.1 ml)
  • 关于如何获取EGR1 (B-6): sc-515830的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是EGR1 Antibody (B-6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与EGR1 Antibody (B-6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Egr抗体，包括Egr-1, Egr-2和Egr-3

Egr-1抗体（B-6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的Egr-1蛋白。抗 -Egr-1抗体（B-6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Egr-1是早期生长反应（Egr）转录因子家族的一员，通过与特定DNA序列结合，从而调节靶基因的转录，在细胞分裂和分化等细胞过程中发挥着关键作用。Egr-1（B-6）单克隆抗体主要靶向位于细胞核中的蛋白质，Egr-1在细胞核中作为转录激活因子响应细胞外刺激，这凸显了Egr-1在细胞信号通路中的重要性。Egr-1受多种信号（包括生长因子和压力）快速诱导，并经历复杂的调节机制，包括翻译后修饰（如磷酸化、糖基化等），这些修饰会影响其活性和稳定性。了解Egr-1的功能和调节机制至关重要，因为失调与多种疾病（包括癌症和心血管疾病）有关，这使得Egr-1 (B-6)抗体成为研究人员研究这些关键生物学过程的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EGR1 参考文献:

  1. Egr-1 负向调节心室细胞中钙调蛋白的表达和钙动力学  |  Kasneci, A., et al. 2009. Cardiovasc Res. 81: 695-702. PMID: 19103607
  2. Egr-1 上调 Siva-1 的表达并诱导心脏成纤维细胞凋亡  |  Zins, K., et al. 2014. Int J Mol Sci. 15: 1538-53. PMID: 24451137
  3. Egr-1通过PKC/ERK途径介导慢性缺氧诱导的肾间质纤维化  |  Sun, S., et al. 2014. Am J Nephrol. 39: 436-48. PMID: 24819335
  4. Egr-1通过Atg4B调节辐照诱导的自噬，促进肝癌细胞的放射抗性。  |  Peng, WX., et al. 2017. Oncogenesis. 6: e292. PMID: 28134935
  5. Egr-1可维持NSC增殖，其过表达可抵消表皮生长因子撤出引发的细胞周期退出。  |  Cera, AA., et al. 2018. Dev Neurosci. 40: 223-233. PMID: 29975945
  6. Egr-1转激活WNT5A基因表达以抑制葡萄糖诱导的β细胞增殖。  |  Wu, X., et al. 2018. Biochim Biophys Acta Gene Regul Mech.. PMID: 30025875
  7. 心肌缺血大鼠模型中 Egr-1 和 c-fos 表达的时间变化。  |  Zhai, LQ., et al. 2021. Ann Palliat Med. 10: 1411-1420. PMID: 33183026
  8. Klotho 通过下调 Egr-1 防止糖尿病肾病的上皮-间质转化。  |  Li, Y., et al. 2021. BMJ Open Diabetes Res Care. 9: PMID: 34099438
  9. 依赖于 JNK 的 EGR-1 磷酸化和核易位可促进心肌细胞凋亡。  |  Zhou, J., et al. 2022. Apoptosis. 27: 246-260. PMID: 35103892
  10. 双重敲除 Egr-1 和 RCAN1 的小鼠在铜绿假单胞菌肺部感染过程中的炎症表现减弱。  |  Pang, Z. and Lin, TJ. 2023. Immunobiology. 228: 152377. PMID: 36933529

订购信息

产品名称产品编号规格价格数量收藏夹

EGR1 抗体 (B-6)

sc-515830
200 µg/ml
RMB2377.00

EGR1 (B-6): m-IgG Fc BP-HRP 套装

sc-531532
200 µg Ab; 10 µg BP
RMB2662.00

EGR1 (B-6): m-IgGκ BP-HRP 套装

sc-525243
200 µg Ab, 40 µg BP
RMB2662.00

EGR1 (B-6): m-IgG1 BP-HRP 套装

sc-544609
200 µg Ab; 20 µg BP
RMB2662.00

EGR1 抗体 (B-6) X

sc-515830 X
200 µg/0.1 ml
RMB2377.00

EGR1 抗体 (B-6) AC

sc-515830 AC
500 µg/ml, 25% agarose
RMB3129.00

EGR1 抗体 (B-6) HRP

sc-515830 HRP
200 µg/ml
RMB2377.00

EGR1 抗体 (B-6) FITC

sc-515830 FITC
200 µg/ml
RMB2482.00

EGR1 抗体 (B-6) PE

sc-515830 PE
200 µg/ml
RMB2580.00

EGR1 抗体 (B-6) Alexa Fluor® 488

sc-515830 AF488
200 µg/ml
RMB2685.00

EGR1 抗体 (B-6) Alexa Fluor® 546

sc-515830 AF546
200 µg/ml
RMB2685.00

EGR1 抗体 (B-6) Alexa Fluor® 594

sc-515830 AF594
200 µg/ml
RMB2685.00

EGR1 抗体 (B-6) Alexa Fluor® 647

sc-515830 AF647
200 µg/ml
RMB2685.00

EGR1 抗体 (B-6) Alexa Fluor® 680

sc-515830 AF680
200 µg/ml
RMB2685.00

EGR1 抗体 (B-6) Alexa Fluor® 790

sc-515830 AF790
200 µg/ml
RMB2685.00

EGR1 (B-6) 中和勝肽

sc-515830 P
100 µg/0.5 ml
RMB511.00

Hello I am planning on buying this but my target is zebrafish egr1(Egr-1 (B-6): sc-515830 mouse monoclonal antibody). The paper i read had good bands is there any comment on cross reactivity on zebrafish?

Asked by: Varsha
Thank you for your question. We have not tested on Zebra fish. There is a 57% homology for Zebra fish and we do not recommend a specie unless the homology is 95% or higher.
Answered by: BlakeJ
Date published: 2024-03-28

Dear, I bought Egr1 antibody (sc-515830) several months ago. But it didn't give me band for WB.  I want to conjugate PE(Or FITC) to this Egr1 antibody. for flow cytometry. Could you send me the protocol about how to conjugate fluorophore to this product?

Asked by: Lingtian
Please contact Technical Service for more details.
Answered by: Tech Sales
Date published: 2021-06-25

I was wondering if sc-515830 X have been tested for EMSA? I tried using the antibody for an EMSA with HEK293T extracts and probe with EGR1 binding site, however, could not see a supershift.

Asked by: CJ12
We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please reach out to your local distributor in the UK or contact Technical Service directly. We are happy to help you optimize and troubleshoot the antibody to make sure you have success with the antibody.
Answered by: Tech Service
Date published: 2020-01-10

I used the Ab (Lot L0816) at 1:200 on total lysates from HFF – no bands whatsoever. The internal control for loading (tubulin) was clear and strong. Any suggestions?

Asked by: Roumen Pankov
Dear Ruomen Pankov, thank you very much for your question. We are happy to help you to troubleshoot the experiment. Please contact our Technical Service Department by email to [email protected] with your protocol details.
Answered by: Technical Support Europe
Date published: 2018-05-23

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if Egr-1 (B-6): sc-515830 mouse monoclonal antibody will work?

Asked by: jenniferc
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2018-10-24
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Rated 5 out of 5 by from Great featuresFits the description. Detects correct band without blocking.
Date published: 2019-01-22
Rated 1 out of 5 by from 谱带不清晰左一为Marker，其它为Hela细胞用Ripa（强）裂解得到的谱带，目标谱带应该在图中第二排谱带的位置，但是不明显，而且杂带多。
Date published: 2018-04-12
Rated 3 out of 5 by from Weak band detected via western blotWeak band detected at ~55 kDa in U87MG and U118MG glioblastoma lysates. Evaluated via western blot.
Date published: 2017-11-29
Rated 1 out of 5 by from ungeeignet für IHC an GefrierschnittenDieser Antikörper ist nicht für Immunhistochemie an PFA fixierten Gefrierschnitten von Ratten geeignet. Er zeigt entweder kein signal (Standardprotokoll mit Übernachtinkubation im 1. AK) oder ein unspezifische Färbung (6 Tage Langzeitinkubation mit biotinylierter Tyramin Verstärkung)
Date published: 2017-09-13
Rated 3 out of 5 by from Work for WBUse siEgr MEF cell samples, Ab dilute to 1:500, see single but not so strong band.
Date published: 2017-08-17
Rated 5 out of 5 by from strong bandThe molecular weight of 82 kDa is the expected molecular weight of Egr-1 after post-translational modifications. Technical service representative suggested the bands that I got are the truncated, non-phosphorylated protein, which could be around 54-58 kDa.
Date published: 2017-07-17
Rated 4 out of 5 by from Good antibody for Western blotI bought this antibody to test gRNAs against EGR-1. Worked well in human cells. It is able to detect the endogenous protein. Band gets weaker when the population is transfected with gRNAs for knockout.
Date published: 2017-07-03
Rated 5 out of 5 by from good WB resultThis Egr-1 antibody is very good, the band is pretty strong at the right size.
Date published: 2017-05-23
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Egr-1 抗体 (B-6) is rated 3.8 out of 5 by 11.
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