Date published: 2025-9-7
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EGR1 Antibody (S-25) - Western Blotting - Image 34398
Egr-1 抗体 (S-25): sc-101033. 对 Raw 264. 7 全细胞裂解液中 Egr-1 的表达进行 Western 印迹分析.
EGR1 Antibody (S-25) - Western Blotting - Image 34398
Egr-1 抗体 (S-25): sc-101033. 对 Raw 264. 7 全细胞裂解液中 Egr-1 的表达进行 Western 印迹分析.
EGR1 Antibody (S-25) - Immunohistochemistry - Image 36265
Egr-1 抗体 (S-25): sc-101033. 福尔马林固定, 石蜡包埋的人胎盘组织的免疫过氧化物酶染色, 显示核定位.
EGR1 Antibody (S-25) - Immunofluorescence - Image 35768
Egr-1 抗体 (S-25): sc-101033. 免疫荧光染色多聚甲醛固定的 HeLa 细胞, 显示核定位.
Egr-1 抗体 (S-25): sc-101033. 对 Raw 264. 7 全细胞裂解液中 Egr-1 的表达进行 Western 印迹分析.

EGR1 抗体 (S-25): sc-101033

3.7(7)
寫評論提問

说明书
  • EGR1抗体(S-25)是小鼠单克隆IgG2a κ, 在24篇文献中引用，规格为100 µg/ml
  • 针对部分重组蛋白，其氨基酸序列在Egr-1的human起源的444-544范围内
  • 推荐用于 mouse, rat 和 human 来源的Egr-1 p82 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取EGR1 (S-25): sc-101033的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成EGR1 抗体 (S-25)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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基因信息
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研究信息

関連項目

此外 Egr 抗体 包括 Egr-1, Egr-2和Egr-3

Egr-1抗体（S-25）是一种IgG2a κ小鼠单克隆Egr-1抗体，可通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）、免疫组织化学法（IHC(P)）和酶联免疫吸附测定法（ELISA）检测小鼠、大鼠和人源性的Egr-1 p82。Egr-1抗体（S-25）为非偶联型抗Egr-1抗体。Egr-1、Egr-2、Egr-3和Egr-4是核转录因子，属于Egr C2H2型锌指蛋白家族，每个都含有三个C2H2型锌指。作为即早蛋白，Egr转录因子可被多种细胞外刺激迅速诱导。它们受到转录、翻译和翻译后（包括糖基化、磷酸化和氧化还原）机制以及蛋白质-蛋白质相互作用等多个层面的严格调控。Egr-1与DNA序列5'-CGCCCCCGC-3'（Egr位点）结合，从而激活靶基因的转录，这些靶基因编码的产物是细胞有丝分裂和分化所必需的。Egr-2与HoxA4启动子区域的特定DNA位点结合。Egr-2缺陷会导致先天性低髓鞘神经病（也称为Charcot-Marie-Tooth病）和Dejerine-Sottas神经病（也称为遗传性运动感觉神经病III）。Egr-3参与肌梭的发育，并在T细胞激活后20分钟内表达。Egr-4与Egr共识基序GCGTGGGCG结合，起转录阻遏物的作用，并表现出自调节活性，以剂量依赖的方式下调其自身基因启动子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EGR1 抗体 (S-25) 参考文献:

  1. 在 MCF-7 细胞中，17beta-雌二醇通过依赖于 ELK-1 的丝裂原活化蛋白激酶磷酸化激活 Egr-1。  |  Chen, CC., et al. 2004. J Cell Biochem. 93: 1063-74. PMID: 15449318
  2. 血管平滑肌细胞中血管生成因子 CCN1 在拉伸诱导下的表达是由 Egr-1 介导的。  |  Grote, K., et al. 2004. J Biol Chem. 279: 55675-81. PMID: 15492009
  3. HGF 通过 MAP 激酶依赖性信号通路和 Egr-1 的诱导，诱导黑色素瘤细胞中纤维粘连蛋白基质的合成。  |  Gaggioli, C., et al. 2005. Oncogene. 24: 1423-33. PMID: 15608673
  4. 在胰腺α细胞中，Egr-1与胰岛特异性反应元件的相互作用对胰岛素依赖性胰高血糖素基因的转录至关重要。  |  Leung-Theung-Long, S., et al. 2005. J Biol Chem. 280: 7976-84. PMID: 15611055
  5. MAPK 通路和 Egr-1 介导了糖皮质激素与应激相关的行为效应。  |  Revest, JM., et al. 2005. Nat Neurosci. 8: 664-72. PMID: 15834420
  6. 神经系统中的 Egr 转录因子。  |  Beckmann, AM. and Wilce, PA. 1997. Neurochem Int. 31: 477-510; discussion 517-6. PMID: 9307998
  7. 人类锌指蛋白 EGR-4 是一种自动调节转录抑制因子。  |  Zipfel, PF., et al. 1997. Biochim Biophys Acta. 1354: 134-44. PMID: 9396630

订购信息

产品名称产品编号规格价格数量收藏夹

EGR1 抗体 (S-25)

sc-101033
100 µg/ml
RMB2504.00

With which diluent should I use for diluting primary antibody, milk or BSA?

Asked by: ww20
Thank you for your question. We recommend UltraCruz® Blocking Reagent: sc-516214, which is the blocking reagent with which all of our antibodies are established. Alternatively, a blocking reagent based on milk powder is more suitable for blots with strong signal and high background, whereas a BSA buffer is better used for weak but clean signals.
Answered by: Tech Support Europe
Date published: 2024-08-26

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 3 out of 5 by from It works for western blotting.It detected a band with the expected molecular mass of EGR1 in total protein extracts from HeLa cells (1:500 in 5% milk/TBS-0.1% Tween, overnight at 4C).
Date published: 2018-02-15
Rated 1 out of 5 by from Didnot work for WBNo band detected in MDA-MB-231 cell line. Used the antibody at 1:1000 dilution
Date published: 2017-06-07
Rated 5 out of 5 by from Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Great for WB.
Date published: 2017-01-13
Rated 5 out of 5 by from The results of western blot were greatThe results of western blot were great.
Date published: 2015-12-04
Rated 4 out of 5 by from This has worked well in WB&IFThis has worked well in WB&IF.
Date published: 2015-10-21
Rated 5 out of 5 by from Favorable signal achieved in Raw 264Favorable signal achieved in Raw 264.7 whole cell lysate. -SCBT QC
Date published: 2015-05-14
Rated 3 out of 5 by from Produced acceptable immunoperoxidase nuclearProduced acceptable immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-05-08
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EGR1 抗体 (S-25) is rated 3.7 out of 5 by 7.
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