Date published: 2025-9-7

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Eg5 抗体 (A-2): sc-365593

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说明书
  • Eg5 抗体 A-2 是小鼠单克隆 IgG2a κ,Eg5抗体, 在3篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列753-1052位于mouse物种的Eg5的C-terminus
  • Eg5 抗体 (A-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测mouse 来源的 kinesin-related motor protein Eg5
  • 抗Eg5抗体(A-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Eg5 (A-2): sc-365593免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 Eg5 抗体 (A-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Eg5 抗体 (A-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Eg5抗体(A-2)是一种IgG2a κ小鼠单克隆Eg5抗体(也称为Eg5抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠来源的Eg5蛋白。Eg5抗体(A-2)既可以是非偶联的抗Eg5抗体形式,也可以是多种偶联形式的抗Eg5抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。真核生物含有一个基于微管的马达蛋白超家族,其中包括驱动蛋白和许多相关蛋白,这些蛋白被认为参与各种形式的细胞内运动,包括细胞分裂和细胞器运输。KIF11(也称为驱动蛋白家族成员11、Eg5或TRIP5)是BimC驱动蛋白家族中一种慢速的、正端导向的基于微管的马达,对于真核细胞分裂过程中的双极纺锤体形成至关重要。当KIF11的表达被阻断时,中心体迁移停止,细胞在单星状微管阵列中有丝分裂停滞。KIF11在S期丝氨酸上发生磷酸化,在有丝分裂期间丝氨酸和苏氨酸927上也发生磷酸化,这调节了Eg5与纺锤体装置的关联(可能是在早期前期)。已知KIF11还是甲状腺受体相互作用蛋白(Trip)家族的成员,并且仅在甲状腺激素存在的情况下与甲状腺激素受体相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Eg5 抗体 (A-2) 参考文献:

  1. KIF11 是一种癌基因,与乳腺癌的不良预后有关。  |  Zhou, J., et al. 2019. Cancer Res Treat. 51: 1207-1221. PMID: 30590004
  2. KIF11 作为小鼠曲细精管横截面内精子发生的潜在标记物  |  Hara-Yokoyama, M., et al. 2019. J Histochem Cytochem. 67: 813-824. PMID: 31424977
  3. TRIM8 与 KIF11 和 KIFC1 相互作用,控制双极纺锤体的形成和染色体的稳定性。  |  Venuto, S., et al. 2020. Cancer Lett. 473: 98-106. PMID: 31904480
  4. 驱动蛋白家族成员 11 (Kif11) 相关家族性渗出性玻璃体视网膜病变的小鼠模型。  |  Wang, Y., et al. 2020. Hum Mol Genet. 29: 1121-1131. PMID: 31993640
  5. KIF11的间期池定位在初级纤毛的基部体,KIF11表达的减少会改变纤毛的动态。  |  Zalenski, AA., et al. 2020. Sci Rep. 10: 13946. PMID: 32811879
  6. KIF11 抑制剂 filanesib 和 ispinesib 可抑制脑膜瘤在体外和体内的生长。  |  Jungwirth, G., et al. 2021. Cancer Lett. 506: 1-10. PMID: 33652084
  7. ASPM 通过稳定 KIF11 促进无性甲状腺癌的迁移和侵袭。  |  Fang, Q., et al. 2023. Cell Biol Int. 47: 1209-1221. PMID: 36883909
  8. 环维菌素D通过抑制KIF11-CDC25C-CDK1-CyclinB1 G2/M期转换调控网络和NFκB/JNK信号通路,抑制非小细胞肺癌细胞的生长和进展。  |  Xue, T., et al. 2023. Int J Oncol. 62: PMID: 36929198
  9. LncRNA NEAT1 通过 KIF11 依赖性抑制 CDKN2A 抑制肝细胞癌的细胞衰老。  |  Chen, D., et al. 2023. Clin Transl Med. 13: e1418. PMID: 37752791
  10. KIF11 UFMylation 维持感光细胞纤毛完整性和视网膜稳态  |  Ran, J., et al. 2024. Adv Sci (Weinh). 11: e2400569. PMID: 38666385

订购信息

产品名称产品编号规格价格数量收藏夹

Eg5 抗体 (A-2)

sc-365593
200 µg/ml
RMB2377.00

Eg5 (A-2): m-IgG Fc BP-HRP 套装

sc-529400
200 µg Ab; 10 µg BP
RMB2662.00

Eg5 (A-2): m-IgGκ BP-HRP 套装

sc-522281
200 µg Ab, 40 µg BP
RMB2662.00

Eg5 (A-2): m-IgG2a BP-HRP 套装

sc-547310
200 µg Ab; 10 µg BP
RMB2662.00

Eg5 抗体 (A-2) AC

sc-365593 AC
500 µg/ml, 25% agarose
RMB3129.00

Eg5 抗体 (A-2) HRP

sc-365593 HRP
200 µg/ml
RMB2377.00

Eg5 抗体 (A-2) FITC

sc-365593 FITC
200 µg/ml
RMB2482.00

Eg5 抗体 (A-2) PE

sc-365593 PE
200 µg/ml
RMB2580.00

Eg5 抗体 (A-2) Alexa Fluor® 488

sc-365593 AF488
200 µg/ml
RMB2685.00

Eg5 抗体 (A-2) Alexa Fluor® 546

sc-365593 AF546
200 µg/ml
RMB2685.00

Eg5 抗体 (A-2) Alexa Fluor® 594

sc-365593 AF594
200 µg/ml
RMB2685.00

Eg5 抗体 (A-2) Alexa Fluor® 647

sc-365593 AF647
200 µg/ml
RMB2685.00

Eg5 抗体 (A-2) Alexa Fluor® 680

sc-365593 AF680
200 µg/ml
RMB2685.00

Eg5 抗体 (A-2) Alexa Fluor® 790

sc-365593 AF790
200 µg/ml
RMB2685.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with Eg5 (A-2): sc-365593 monoclonal antibody?

Asked by: Cweed
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from western blotI used this antibody for western blot on breast cancer cell samples (1:200), it works well.
Date published: 2021-07-22
Rated 4 out of 5 by from Good for IFThis Eg5 Antibody (A-2), sc-365593, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 5 out of 5 by from were greatWorked well in WB in mouse liver tissue at 1:200 dilution, strong wb with low background, Recommend to everyone!
Date published: 2017-03-18
Rated 5 out of 5 by from Can be used in many cell lines.Antibody gives very good signal,can be used in many cell lines.
Date published: 2017-02-13
Rated 5 out of 5 by from Works well and the background is clearMethods The cellular localization of Eg5 Antibody in mouse kidney tissue was detected by immunoblot and immunohistochemistry. We got specific signal for the protein at the right molecular weight.
Date published: 2017-02-09
Rated 5 out of 5 by from Produced positive Western Blot data of Eg5Produced positive Western Blot data of Eg5 expression in M1 whole cell lysate. -SCBT QC
Date published: 2015-01-08
Rated 5 out of 5 by from Nice Western Blot data of Eg5 expression in M1Nice Western Blot data of Eg5 expression in M1, MCF7, A-431, Jurkat, Raji and K-562 whole cell lysates. -SCBT QC
Date published: 2014-11-11
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Eg5 抗体 (A-2) is rated 4.7 out of 5 by 7.
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