Date published: 2025-9-7

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Eg5 抗体 (F-1): sc-374212

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说明书
  • Eg5抗体(F-1)是小鼠单克隆IgG1 κ, 在2篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Eg5的C-terminus的氨基酸1029-1057之间
  • Eg5 抗体 (F-1) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 Eg5
  • 抗Eg5抗体(F-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Eg5 (F-1): sc-374212免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Eg5 Antibody (F-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Eg5 Antibody (F-1) 打包提供(请参阅下面的订购信息)。

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関連項目

Eg5抗体(F-1)是一种IgG1 κ小鼠单克隆Eg5抗体,可通过WB、IP、IF和ELISA检测人源Eg5。Eg5抗体(F-1)既可以是非偶联的抗Eg5抗体形式,也可以是多种偶联形式的抗Eg5抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。真核生物含有一个基于微管的马达蛋白超家族,包括驱动蛋白和许多相关蛋白,这些蛋白被认为参与各种形式的细胞内运动,包括细胞分裂和细胞器运输。Eg5(也称为驱动蛋白样蛋白KIF11或TRIP5)是BimC驱动蛋白家族中一种慢速的、正端导向的基于微管的马达,对于真核细胞分裂过程中的双极纺锤体形成至关重要。当Eg5的表达被阻断时,中心体迁移停止,细胞在单星状微管阵列中有丝分裂停滞。Eg5在S期丝氨酸上发生磷酸化,在有丝分裂期间丝氨酸和苏氨酸927上也发生磷酸化,这调节了Eg5与纺锤体装置的关联(可能是在早期前期)。已知Eg5还是甲状腺受体相互作用蛋白(Trip)家族的成员,并且仅在甲状腺激素存在的情况下与甲状腺激素受体相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Eg5 抗体 (F-1) 参考文献:

  1. 驱动蛋白相关蛋白 HSET 在哺乳动物有丝分裂纺锤体中对抗 Eg5 的活性并交联微管。  |  Mountain, V., et al. 1999. J Cell Biol. 147: 351-66. PMID: 10525540
  2. 有丝分裂驱动蛋白 Eg5 的机理分析。  |  Cochran, JC., et al. 2004. J Biol Chem. 279: 38861-70. PMID: 15247293
  3. AURKA, DLGAP5, TPX2, KIF11 和 CKAP5:五个特定的有丝分裂相关基因与非小细胞肺癌患者的不良预后相关。  |  Schneider, MA., et al. 2017. Int J Oncol. 50: 365-372. PMID: 28101582
  4. 对 KIF11 相关视网膜病变表型谱的新认识,包括一种新形式的视网膜纤毛病。  |  Birtel, J., et al. 2017. Invest Ophthalmol Vis Sci. 58: 3950-3959. PMID: 28785766
  5. 在一名患有家族性渗出性玻璃体视网膜病变并伴有小头畸形的患者身上检测到 KIF11 嵌合体并对其进行量化。  |  Karjosukarso, DW., et al. 2018. Eur J Hum Genet. 26: 1819-1823. PMID: 30181612
  6. 与新型肾脏系统受累有关的 KIF11 变异--两例扩大了伴有或不伴有脉络膜视网膜病变、淋巴水肿或智力发育受损的小头畸形的表型范围。  |  Gonzalez, T., et al. 2025. Am J Med Genet A. 197: e63903. PMID: 39404449
  7. 在有丝分裂过程中,HPV16 的进入需要动力蛋白进行负端运输,并利用驱动蛋白 Kif11 沿着微管进行正端运输。  |  Keiffer, TR., et al. 2025. J Virol. 99: e0093724. PMID: 39629998
  8. 两类蛋白质与甲状腺激素受体的相互作用取决于甲状腺激素的存在或不存在。  |  Lee, JW., et al. 1995. Mol Endocrinol. 9: 243-54. PMID: 7776974
  9. p34cdc2的磷酸化调节人Eg5的纺锤体缔合作用,Eg5是一种驱动蛋白相关电机,对体内双极纺锤体的形成至关重要。  |  Blangy, A., et al. 1995. Cell. 83: 1159-69. PMID: 8548803
  10. 小鼠基因组中 16 个新的驱动蛋白超家族(KIF)蛋白的鉴定与分类  |  Nakagawa, T., et al. 1997. Proc Natl Acad Sci U S A. 94: 9654-9. PMID: 9275178
  11. 拓展 HsEg5 在细胞周期的有丝分裂期和后有丝分裂期的作用。  |  Whitehead, CM. and Rattner, JB. 1998. J Cell Sci. 111 (Pt 17): 2551-61. PMID: 9701554
  12. 有丝分裂运动蛋白 Eg5 在有丝分裂后神经元中的表达:对神经元发育的影响。  |  Ferhat, L., et al. 1998. J Neurosci. 18: 7822-35. PMID: 9742151

订购信息

产品名称产品编号规格价格数量收藏夹

Eg5 抗体 (F-1)

sc-374212
200 µg/ml
RMB2377.00

Eg5 (F-1): m-IgG Fc BP-HRP 套装

sc-529688
200 µg Ab; 10 µg BP
RMB2662.00

Eg5 (F-1): m-IgGκ BP-HRP 套装

sc-522660
200 µg Ab, 40 µg BP
RMB2662.00

Eg5 (F-1): m-IgG1 BP-HRP 套装

sc-543636
200 µg Ab; 20 µg BP
RMB2662.00

Eg5 抗体 (F-1) AC

sc-374212 AC
500 µg/ml, 25% agarose
RMB3129.00

Eg5 抗体 (F-1) HRP

sc-374212 HRP
200 µg/ml
RMB2377.00

Eg5 抗体 (F-1) FITC

sc-374212 FITC
200 µg/ml
RMB2482.00

Eg5 抗体 (F-1) PE

sc-374212 PE
200 µg/ml
RMB2580.00

Eg5 抗体 (F-1) Alexa Fluor® 488

sc-374212 AF488
200 µg/ml
RMB2685.00

Eg5 抗体 (F-1) Alexa Fluor® 546

sc-374212 AF546
200 µg/ml
RMB2685.00

Eg5 抗体 (F-1) Alexa Fluor® 594

sc-374212 AF594
200 µg/ml
RMB2685.00

Eg5 抗体 (F-1) Alexa Fluor® 647

sc-374212 AF647
200 µg/ml
RMB2685.00

Eg5 抗体 (F-1) Alexa Fluor® 680

sc-374212 AF680
200 µg/ml
RMB2685.00

Eg5 抗体 (F-1) Alexa Fluor® 790

sc-374212 AF790
200 µg/ml
RMB2685.00

Eg5 (F-1) 中和勝肽

sc-374212 P
100 µg/0.5 ml
RMB511.00

What is the likelihood this antibody would work in zebrafish?

Asked by: SXR1
Thank you for your question. Anti-Eg5 Antibody (F-1): sc-374212 is only recommended for detection of Eg5 of human origin by WB, IP, IF and ELISA. We do not recommend it for detecting its target protein in any other species. It is unlikely that this product would detect SXR1 in Zebrafish.
Answered by: Tech Support Europe
Date published: 2021-08-27

What application is the blocking peptide sc-374212 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Good for IFThis Eg5 antibody shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2017-11-06
Rated 5 out of 5 by from It's a magnificent antibodyEg5 antibody gives very good signal. We use many times, bought two antibodies but different batches this time, the quality is stable, we will continue to support the purchase of your company's products.
Date published: 2017-02-09
Rated 5 out of 5 by from excellent antibodyIt gives clear staining for nuclear extracts in western blot; safely WIll buy it again.
Date published: 2016-12-22
Rated 5 out of 5 by from Beautiful immunofluorescence mitiotic spindleBeautiful immunofluorescence mitiotic spindle poles staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-10
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Eg5 抗体 (F-1) is rated 5.0 out of 5 by 5.
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