Date published: 2025-9-6

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EF-2 抗体 (H-8): sc-390014

5.0(4)
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说明书
  • EF-2抗体(H-8)是小鼠单克隆IgG2b (kappa轻链),规格为200 µg/ml
  • 特异性抗原位于human物种的EF-2的C-terminus附近的氨基酸811-849之间
  • 推荐用于 mouse, rat 和 human 来源的EF-2 WB, IP, IF, IHC(P) 和 ELISA检测
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 EF-2 抗体 (H-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与EF-2 抗体 (H-8) 组合成套装提供。 (请参阅下面的订购信息)。

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    関連項目

    在核糖体上的蛋白质生物合成过程中,两个延长因子(EF)EF-Tu和EF-2参与延长阶段,它们的功能周期依赖于GTP的结合及其水解。EF-Tu(也称为线粒体前体p43)和EF-2是具有翻译必需功能的多结构域GTP酶,它们都结合在核糖体上的同一位置,在该位置上它们的低内在GTP酶活性受到强烈刺激。EF-Tu在将氨酰基-tRNA快速准确地递送至翻译中的核糖体中起着核心作用。此外,EF-Tu还保护氨基酯键免受水解,直到mRNA上的密码子与tRNA上的反密码子之间实现正确匹配。EF-2支持tRNA和mRNA在核糖体上的易位,从而可以暴露新的密码子以进行解码。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    EF-2 抗体 (H-8) 参考文献:

    1. 伸长因子2激酶调节人胶质母细胞瘤细胞的自噬。  |  Wu, H., et al. 2006. Cancer Res. 66: 3015-23. PMID: 16540650
    2. 伸长因子2激酶通过抑制蛋白质合成而不诱导自噬来促进细胞存活。  |  Moore, CE., et al. 2016. Cell Signal. 28: 284-93. PMID: 26795954
    3. 真核细胞伸长因子2激酶通过PI3K/Akt和STAT3促进肝细胞癌的血管生成。  |  Zhou, Y., et al. 2020. Int J Cancer. 146: 1383-1395. PMID: 31286509
    4. 靶向真核生物的延伸因子2激酶抑制卵巢癌的生长和腹膜转移。  |  Erdogan, MA., et al. 2021. Cell Signal. 81: 109938. PMID: 33539938
    5. 真核细胞伸长因子2激酶通过翻译CD36调节泡沫细胞的形成。  |  Fernando, S., et al. 2022. FASEB J. 36: e22154. PMID: 35032419
    6. 延伸因子2激酶是CD8+ T细胞命运和抗肿瘤免疫的关键决定因素。  |  Das, JK., et al. 2022. Sci Adv. 8: eabl9783. PMID: 35108044
    7. 真核细胞延伸因子 2 激酶抑制剂 A484954 可抑制血管周围交感神经刺激引起的离体肾动脉血管收缩。  |  Kodama, T., et al. 2022. Eur J Pharmacol. 926: 175042. PMID: 35598844
    8. 真核生物的延伸因子2激酶活性是Rpl24Bst小鼠表型的必要条件。  |  Knight, JRP., et al. 2022. J Invest Dermatol. 142: 3346-3348.e1. PMID: 35850210
    9. ADP通过钙调蛋白增强真核细胞伸长因子2激酶的变构激活。  |  Piserchio, A., et al. 2023. Proc Natl Acad Sci U S A. 120: e2300902120. PMID: 37068230
    10. 延伸因子2激酶:组织特异性同工型存在的免疫学证据。  |  Hait, WN., et al. 1996. FEBS Lett. 397: 55-60. PMID: 8941713

    订购信息

    产品名称产品编号规格价格数量收藏夹

    EF-2 抗体 (H-8)

    sc-390014
    200 µg/ml
    RMB2377.00

    EF-2 (H-8): m-IgGκ BP-HRP 套装

    sc-535580
    200 µg Ab; 40 µg BP
    RMB2662.00

    EF-2 (H-8): m-IgG2b BP-HRP 套装

    sc-549450
    200 µg Ab; 10 µg BP
    RMB2662.00

    EF-2 (H-8) 中和勝肽

    sc-390014 P
    100 µg/0.5 ml
    RMB511.00

    What application is the blocking peptide sc-390014 P appropriate for?

    Asked by: Germaine
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from Good specificity in WB in HLGood specificity in WB in HL-60 whole cell lysates. -SCBT QC
    Date published: 2015-05-15
    Rated 5 out of 5 by from Strong staining in WB in PCStrong staining in WB in PC-12 and BC3H1 whole cell lysates. -SCBT QC
    Date published: 2015-05-10
    Rated 5 out of 5 by from Positive signal in WB in HeLa whole cell lysatePositive signal in WB in HeLa whole cell lysate. -SCBT QC
    Date published: 2015-02-11
    Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmicExceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human colon tissue. -SCBT QC
    Date published: 2015-02-02
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    EF-2 抗体 (H-8) is rated 5.0 out of 5 by 4.
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